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ID   PI258      preliminary; circular DNA; SYN; 1148 BP.
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AC   M15526;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Staphylococcus plasmid vector pI258 - incomplete.
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KW   cloning vector.
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OS   Staphylococcus aureus
OC   Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
OC   Staphylococcaceae.
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RN   [1]
RP   1-1148
RC   pI258 from S. aureus, amp gene
RC   pWN101 from pC194 & pAO7
RA   Wang P.Z., Novick R.P.;
RT   "Nucleotide sequence and expression of the beta-lactamase gene from
RT   Staphylococcus aureus plasmid pI258 in Escherichia coli, Bacillus
RT   subtilis, and Staphylococcus aureus";
RL   J. Bacteriol. 169:1763-1766(1987).
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RN   [2]
RC   pI258 from S. aureus, amp gene
RC   pII147 from S. aureus, amp gene
RA   Novick R.P., Richmond M.H.;
RT   ;
RL   J. Bacteriol. 90:467-480(1965).
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CC   Staphylococcus beta-lactamase gene is 1146 bp.
CC   NM (pI258)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Staphylococcus aureus)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. S. aureus, amp gene
FT                   -> pI258"
FT   misc_binding    1..5
FT                   /note="SIT HindIII"
FT   promoter        69..74
FT                   /note="PRO S. aureus beta-lactamase gene -35 region"
FT   promoter        93..98
FT                   /note="PRO S. aureus beta-lactamase gene -10 region"
FT   mRNA            105..>1148
FT                   /note="S. aureus beta-lactamase mRNA"
FT   CDS             140..985
FT                   /note="ANT S. aureus beta-lactamase gene (bla);
FT                   precursor; EC3.5.2.6; TTG start codon"
FT   sig_peptide     140..211
FT                   /note="S. aureus beta-lactamase signal peptide"
FT   CDS             complement(0..0)
FT                   /note="ANT S. aureus beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   mat_peptide     212..982
FT                   /note="S. aureus beta-lactamase mature peptide"
FT   misc_binding    839..839
FT                   /note="SIT XbaI"
FT   misc_binding    872..872
FT                   /note="SIT HaeIII"
FT   misc_binding    1098..1098
FT                   /note="SIT TaqI"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus aureus"
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SQ   Sequence 1148 BP; 460 A; 141 C; 162 G; 385 T; 0 other;
     agcttactat gccattatta ataacttagc catttcaaca ccttctttca aatatttata
     ataaactatt gacaccgata ttacaattgt aatattattg atttataaaa attacaactg
     taatatcgga gggtttattt tgaaaaagtt aatattttta attgtaattg ctttagtttt
     aagtgcatgt aattcaaaca gttcacatgc caaagagtta aatgatttag aaaaaaaata
     taatgctcat attggtgttt atgctttaga tactaaaagt ggtaaggaag taaaatttaa
     ttcagataag agatttgcct atgcttcaac ttcaaaagcg ataaatagtg ctattttgtt
     agaacaagta ccttataata agttaaataa aaaagtacat attaacaaag atgatatagt
     tgcttattct cctattttag aaaaatatgt aggaaaagat atcactttaa aagcacttat
     tgaggcttca atgacatata gtgataatac agcaaacaat aaaattataa aagaaatcgg
     tggaatcaaa aaagttaaac aacgtctaaa agaactagga gataaagtaa caaatccagt
     tagatatgag atagaattaa attactattc accaaagagc aaaaaagaca cttcaacacc
     tgctgctttc ggtaagactt taaataaact tatcgcaaat ggaaaattaa gcaaagaaaa
     caagaaattc ttacttgatt taatgttaaa taataaaagc ggagatactt taattaaaga
     cggtgttcca aaagactata aggttgctga taaaagtggt caagcaataa catatgcttc
     tagaaatgat gttgcttttg tttatcctaa gggccaatct gaacctattg ttttagtcat
     ttttacgaat aaagacaata aaagtgataa gccaaatgat aagttgataa gtgaaaccgc
     caagagtgta atgaaggaat tttaatattc taaatgcata ataaatactg ataacatctt
     atattttgta ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga
     ttttccctct attattttcg agatttattt tcttaattct ctttaacaac tagaatattg
     tatataca
//