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ID   PI3        preliminary; DNA; SYN; 16000 BP.
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AC   ATCC77461;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Deinococcus/E.coli plasmid vector pI3 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pI1 from pKK232-8
RC   pI2 from pS30 & pI1
RC   pI3 from pI2 & pUC18
RC   pI301, pI302, pI303, pI304, pI305, pI314 from pS30
RC   pI3 from pUE10 & pBR322 & cat gene
RC   pI304 from pUE10 & pBR322 & cat gene
RC   pHH1 from pI3
RA   Masters C.I., Minton K.W.;
RT   "Promoter probe and shuttle plasmids for Deinococcus radiodurans";
RL   Plasmid 28:258-261(1992).
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CC   Shuttle vector using ampR selection in E. coli and cmlR selection
CC   in Deinococcus radiodurans. The order of the major features in this
CC   plasmid is: EcoRI/MCS/HindIII - pUE10 replicon - pUE10 promoter -> -
CC   cmlR -> - pMB1 ori - ampR. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: K.W.Minton & C.W.Masters
CC   NM (pI3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Deinococcus radiodurans)(E.coli)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK232-8 remove HindIII-SmaI, MCS
FT                   Klenow fill in:
FT                   -> pI1
FT                   1. pKK232-8 EcoRI, 5' to cat gene
FT                   2. pUE10 EcoRI-EcoRI 2000bp, ori/EcoRI A fragment
FT                   -> pS30 17000bp
FT                   1. pS30 EcoRI-EcoRI 2000bp, ori/EcoRI A fragment
FT                   2. pI1 EcoRI
FT                   -> pI2
FT                   1. pI2 remove small HindIII-EcoRI
FT                   2. pUC18 HindIII-EcoRI, MCS
FT                   -> pI3 16000bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli cat gene"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SstI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Deinococcus radiodurans pUE10"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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