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ID   PI304      preliminary; DNA; SYN; 14500 BP.
AC   ATCC77462;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Deinococcus/E.coli plasmid vector pI304 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pI1 from pKK232-8
RC   pI2 from pS30 & pI1
RC   pI3 from pI2 & pUC18
RC   pI301, pI302, pI303, pI304, pI305, pI314 from pS30
RC   pI3 from pUE10 & pBR322 & cat gene
RC   pI304 from pUE10 & pBR322 & cat gene
RC   pHH1 from pI3
RA   Masters C.I., Minton K.W.;
RT   "Promoter probe and shuttle plasmids for Deinococcus radiodurans";
RL   Plasmid 28:258-261(1992).
CC   Promoter-cloning vector for Deinococcus radiodurans. Inserts with
CC   promoter function permit expression of chloramphenicol resistance. The
CC   order of the major features in this plasmid is: pUE10 replicon -
CC   cmlR - pMB1 ori - ampR. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: K.W.Minton,  H.W.Braymer
CC   NM (pI304)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Deinococcus radiodurans)(E.coli)
CC   CP ()
CC   FN (cloning)(shuttle)(promoter-cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pS30 remove small SmaI-NruI, pUE10
FT                   -> pI304"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   CDS             0..0
FT                   /note="ANT E. coli cat gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Deinococcus radiodurans pUE10"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;