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ID   PIA2       preliminary; circular DNA; SYN; 9300 BP.
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AC   ATCC77160;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pIA2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHSS6-URA from pHSS6 & URA3 gene
RC   pHSS6-Trp from pHSS6 & TRP1 gene
RC   pIA1 from pHSS6-URA & yeast 2 micron
RC   pIA2 from pHSS6-Trp & yeast 2 micron
RC   YEpADE8.1 from pUC18 & ADE8 gene & yeast 2 micron
RC   pHV1 from pUC series & HIS3 & yeast 2 micron
RC   pJM35 from pBR322 & lambda & yeast 2 micron & HIS3 gene
RC   pYc1 from YEp6
RC   pYF177 from yeast 2 micron & HIS3 gene & pBR322
RC   pYF88 from pYF85 & HIS3 gene
RC   pYF92 from pYF86 & HIS3 gene
RC   YEp421 from YEp6 & YEp420
RC   YEp430 from pRB328 & YEp420
RC   YEp4-Sc2703 from YEp4 & HIS3 gene
RC   pC500, pC501 from HIS4 gene & pJC75-58
RC   pHKB52 from yeast 2 micron & LEU2 gene & pBR322
RC   pJDB248 from yeast 2 micron & LEU2 gene & pMB9
RC   pJH4 from yeast 2 micron & LEU2 gene & pUC8
RC   pJZ1 from yeast 2 micron & LEU2 gene
RC   pYF90 from yeast 2 micron & LEU2 gene & pBR322
RC   pYF91 from yeast 2 micron & LEU2 gene & pBR322
RC   pYK71 from YEp13 & T7 promoter phi10/terminator
RC   pYK72 from YEp13 & T7 promoter phi10
RC   pYT7810 from yeast 2 micron & LEU2 gene & pBR325
RC   YEp13S or YEp213 from YEp13
RC   pPL116 from YEp13
RC   pPL160 from YEp13
RC   pPL199, pPL200 from YEp13
RC   YEp13M4 from yeast 2 micron & pUC19 & LEU2 gene
RC   pMCB974 from YEp13 & pUC18
RC   YEp423 from YEp21 & YEp420
RC   YEpLEU2.1 from pUC119 & yeast 2 micron & LEU2 gene
RC   YEpM7 from pUC19 & yeast 2 micron & LEU2 gene
RC   YEp-DE from pUC18 & yeast 2 micron & LEU2 gene
RC   pYTH4 from pBR322 & SUP4.0 & yeast 2 micron
RC   pRC3 from pKC7 & TRP1 gene & ARS1
RC   pRC4 from pRC3 & linker
RC   pRE1052 from TRP1 gene & yeast 2 micron
RC   pTV3 from pUC series & TRP1 gene & yeast 2 micron
RC   YEp9T from pBR322 & TRP1 gene & yeast 2 micron
RC   G18, G23 from pCR1 & URA3 gene & PTY39
RC   pAB18 from YIp5 & yeast 2 micron A
RC   pCOS40 from YIp5 & yeast 2 micron B
RC   pG6 from pBR322 & yeast 2 micron B
RC   pG63 from pG6 & URA3 gene
RC   pG63-11 from pG63
RC   pGN621 from YIp5 & yeast 2 micron B
RC   pHCG3 from pHC79 & pG63-11
RC   pJDB110 from pAT153 & URA3 gene & yeast 2 micron
RC   pSEY8 from pBR322 & URA3 gene & yeast 2 micron
RC   pTB199 from YIp5 & yeast 2 micron B
RC   pTB220, pTB221 from pTB199
RC   pTB224 from pTB220
RC   pTG807 from pBR322 & URA3 gene & yeast 2 micron ori
RC   pUV2 from pUC series & URA3 gene & yeast 2 micron
RC   pYK2060 from YRp16 & pJDB219
RC   YEp103 from YIp5 & yeast 2 micron
RC   YEp16 from pBR322 & URA3 gene & yeast 2 micron
RC   pGT38 from pBR322 & LEU2 & CEN3 & yeast 2 micron
RC   pGT39 from pBR322 & LEU2 & CEN3 & yeast 2 micron
RC   pScT6 from pBR322 & LEU2 gene & yeast 2 micron
RC   YCp2micron from YEp13 & CEN5
RC   pA3 from pBR322 & ADE3 gene & URA3 & yeast 2 micron
RC   pABADE8 from pBR322 & ADE8 gene & LEU2
RC   TLC-1 from YEp13 & CAN1 gene
RC   pJM52 from pJM35 & LEU2 gene
RC   YEp422 from YEp420 & YEp6
RC   YEp431 from YEp420 & pRB328
RC   YEp13a from YEp13
RC   pRB28 from YEp21 & URA3 gene
RC   pRB37, pRB38 from YEp21 & URA3 gene
RC   YEp424 from YEp420 & YEp21
RC   pDA6200 from YEp24 & LYS2 gene
RC   YEp425 from YEp420 & pRB506
RC   pYK2074, pYK2075 from pYK2060 & TRP1 gene
RC   YEp428 from YEp420 & YRp7
RC   YEp433 from YEp420 & pRB315
RC   YEp24cry1 from YEp24 & CRY1 gene
RC   pPL241 from pPL200 & DHFR gene
RC   pJM81 from pJM52 & HSV tk gene
RC   pJM94 from pJM35 & HSV tk gene
RC   pADA1 from pAAH5 & DHFR gene
RC   R5H from YEp24 & PBS2 gene
RC   YEpPBS2 from YEp24 & PBS2 gene
RC   pJR41 from YEp24 & tunicamycin resistance gene
RC   YEp36 from YEp13 & copper MT gene
RC   pUT302 from pEX-2 & Tn5, ble gene
RC   pCH100 from pBR328 & pYcDE-2, ADC1 promoter
RC   pYT11, pYT14 from pBR325 & yeast 2 micron
RC   pYT11-LEU2 from pYT11 & LEU2 gene
RC   pYT14-LEU2 from pYT14 & LEU2 gene
RC   pLG88, pLG89 from pEX-2 & CYC1 promoter & hyg gene
RC   pGH-1 from pLG89 & G418 gene & hyg gene
RC   fdY5 from fd106 & kan gene
RC   PTY39 from pCR1 & yeast 2 micron
RC   pCVneo from pCV20 & Tn5, kan gene
RC   pNF1 from YEp24 & kan gene
RC   pNF2 from pNF1 & linker
RC   pMC3G from pBR322 & URA3 gene & lacZ
RC   pYE13G from pYE13 & kan gene
RC   pRC1 from pKC7 & ARS1/TRP1 gene
RC   pRC2 from YEp13 & pRC1
RC   pRC3 from pRC2
RC   pET13.1 from pBR322 & CUP1 gene
RC   pMP81 from pCR1 & pJDB219
RC   pC1/1 from pSI4
RC   pJDB207 or pMA40 from pAT153 & pJDB219
RC   pJDB209 from pAT153 & pJDB219
RC   pJDB219 from pMB9 & yeast 2 micron
RC   pL623 from pBR325 & LEU2 gene
RC   pMH158 from pJRD158b & LEU2 gene
RC   pMCB803 from pMH158
RC   pMCB967 from pMH158
RC   pMP78-1 from pBR325 & pJDB219
RC   pMP80-3 from pBR325 & pJDB219
RC   pMP80-4 from pBR325 & pJDB219
RC   pPL262 from pC1/1 & pUC19
RC   pPL269 from pC1/1 & pAT153
RC   pPM40 from pBR322 & yeast 2 micron
RC   pROG5 from pBR322 & pJDB219
RC   pSI4 from pBR322 & pJDB219
RC   pSI5 from pBR322 & pJDB219
RC   pY43 from pMB1 & phage f1 ori & LEU2 gene
RC   YEp437 from pSI4 & YEp6
RC   pJDB210, pJDB211 from pAT153 & URA3
RC   pKS2-20 from pJDB219 & YIp5
RC   pYK2024 from pJDB219 & YRp16
RC   YEp436 from pSI4 & YIp5
RC   pAAH5 from YEp13 & ADC1 promoter
RC   pACF301 from LEU2 gene & CYC1 terminator & ADC1 gene & linker
RC   pAD1 from ADC1 promoter & pUC19 & LEU2 gene
RC   pAD4 from pAD1 & ADC1 terminator
RC   pAH5 from pAAH5 & FLP terminator
RC   pAH9 from pAAH5 & FLP terminator
RC   pAH10 from pAAH5 & FLP terminator
RC   pAH21 from pAAH5 & FLP terminator
RC   pAXalpha11 from ADC1 prom & MATalpha1 term & LEU2 gene & pBR322
RC   pADH040-2 from pBR322 & ADC1 promoter
RC   pADH0322-2 from pBR322 & ADC1 promoter
RC   pMA56 from pBR322 & ADC1 prom & FLP term & TRP1 gene
RC   pMAC561 from pBR322 & ADC1 prom & CYC1 term & TRP1 gene
RC   pWY4 from pBR322 & ADC1 prom & ADC1 term & TRP1 gene
RC   pYcDE8 from pBR322 & ADC1 prom & CYC1 term & TRP1 gene
RC   pYcDE-2 from pBR322 & ADC1 prom & CYC1 term & TRP1 gene
RC   pCH98 from pYcDE-2
RC   pRIT10774 from pBR322 & ARG3 prom & ARG3 term & LEU2 gene
RC   pRIT10779 from pBR322 & ARG3 prom & ARG3 term & LEU2 gene
RC   pEX-2 from pBR322 & CYC1 prom & CYC1 term & URA3 gene
RC   pEX-4 from pEX-2 & kan gene
RC   pAC1 from pBR322 & ENO1 prom & ENO1 term & LEU2 gene
RC   pMAC101 from pAC1
RC   pYE8 from interferon gene & pBR322 & GAPDH prom & FLP term & TRP1
RC   pGPD-1 from pBR322 & GPD promoter & TRP1 terminator & TRP1 gene
RC   pGPD-2 from pBR322 & GPD promoter & PGK terminator & TRP1 gene
RC   pMA230 from pBR322 & PGK promoter & LEU2 gene
RC   pMA301 from pBR322 & PGK promoter & LEU2 gene
RC   pMA91 from pMA301 & PGK terminator
RC   YEpIPT from pBR322 & PGK promoter & FLP terminator & TRP1 gene
RC   pMA36 from pBR322 & TRP1 promoter & LEU2 gene
RC   YEp434 from pGM65 & YEp21
RC   YEp435 from pGM65 & YEp21
RC   pLG12 from pLG1/4delta60 & GAL4 gene
RC   pLG1/4delta60 from pBR322 & GAL1 promoter & GAL4 terminator & leu2d
RC   pYMS-3 from pBR322 & GAL1 promoter & ADC1 terminator & leu2d gene
RC   YEpG3 from pMA91
RC   pYRF101 from pLG1/4delta60 & URA3 gene
RC   pYRF102 from pLG12 & URA3 gene
RC   pCGE329 from pBR322 & GAL1 promoter & URA3 gene
RC   pGAL-X/P from pBR322 & GAL1 promoter & URA3 gene
RC   pMAC105 from pBR322 & GAL1-ENO1 promoter & ENO1 terminator & LEU2 gene
RC   YEp213-G from YEp213 & GAL1-GAL10 promoter
RC   pGM65 from YEp420 & GAL1-GAL10 promoter
RC   YEp57 from pAR38 & HIS3 gene
RC   YEp51 from pBR322 & GAL10 promoter & FLP terminator & LEU2 gene
RC   YEp52 from YEp51
RC   YEp55 from YEp51 & GAL10 ATG
RC   YEp62 from pBR322 & GAL10 promoter & lacZ terminator & LEU2 gene
RC   YEpBam from YEp51
RC   YEpUC from YEp51 & pUC8
RC   YEp61 from pBR322 & GAL10 promoter & lacZ terminator & leu2d gene
RC   YEp56 from pAR38 & LYS2 gene
RC   Yep54 from pAR38 & ARS1/TRP1 gene
RC   YEp53 from pAR38 & URA3 gene
RC   pEMBLyex2 from pEMBL series & pEX-2
RC   pEMBLyex4 from pEMBL series & pEX-4
RC   pEMBLyexT3 from pEMBLyex4 & T3 promoter
RC   pEMBLyexT7 from pEMBLyex4 & T7 promoter
RC   pAM81 from pAT77
RC   pAM82 from pAT77
RC   pAT77 from pBR322 & PHO5 promoter & LEU2 gene & ARS1
RC   pJDB207/PHO5 from pJDB207 & PHO5 gene
RC   pYE4 from pBR322 & PHO5 promoter & TRP1 terminator & TRP1 gene
RC   pYE7 from pBR322 & PHO5 promoter & FLP terminator & TRP1 & interferon
RC   p1A1R1-15 from p1A1 & TRP1 gene
RC   pPV1 from pHCG3 & PHO5 promoter
RC   pPV2 from pPV1
RC   SB10-2 from YEp24 & PHO5 promoter
RC   palphaADH2 from pBR322 & ADH2 prom & FLP term & TRP1 & MFalpha1 signal
RC   YEpsec1 from pEMBLyex2 & oligo
RC   pJC1-5 from pBR322 & MFalpha1 promoter/terminator/gene & LEU2 gene
RC   pTG881 from pBR322 & MFalpha1 promoter/signal & PGK terminator & URA3
RC   YEp70alphaT from pBR322 & MFalpha1 prom/signal & FLP term & TRP1
RC   pMFalpha8 from pBR322 & MFalpha1 prom/signal & TRP5 term & TRP1 & ARS1
RC   pSEY210 from pBR322 & MFalpha1 prom/signal & URA3 gene & SUC2
RC   pTG1819 from pBR322 & PGK prom/term & MFalpha1 signal & URA3 & LEU2
RC   pNW033 from pBR322 & PGK prom & pGKL1 & TRP1 term & TRP1 gene
RC   p1A1 from YIp5 & PHO5 promoter/signal & yeast 2 micron
RC   pCGS681 from pBR322 & TPI prom & SUC2 signal & URA3 gene
RC   pIT213 from pBR322 & URA3 gene & yeast 2 micron
RC   pEP100 from pBR327 & LEU2 gene & yeast 2 micron
RC   pJM102 from pBR322 & LEU2 gene & HSV tk gene & yeast 2 micron
RC   pMC1587 from pBR322 & LEU2 gene & yeast 2 micron
RC   pYT760 from pBR322 & LEU2 gene & yeast 2 micron
RC   pRB45 from pBR322 & LEU2 gene & URA3 gene & yeast 2 micron
RC   pJRB10 from pBR322 & leu2d gene & yeast 2 micron
RC   pSL24 from pBR322 & leu2d gene & yeast 2 micron
RC   YEpZ36 from pBR322 & leu2d gene & yeast 2 micron
RC   pMC2010 from pBR322 & TRP1 gene & yeast 2 micron
RC   pCGS139 from pBR322 & URA3 gene & yeast 2 micron
RC   pMC1585 from pBR322 & URA3 gene & yeast 2 micron
RC   pRY140 from pBR322 & URA3 gene & yeast 2 micron
RC   pSEY101 from pCGS139
RC   pLG670-Z from pLG669-Z
RC   YEp363, YEp364, YEp365 from pUC8 & LEU2 gene & yeast 2 micron
RC   YEp363R, YEp364R, YEp365R from pUC8 & LEU2 gene & yeast 2 micron
RC   YEp366, YEp367, YEp368 from pUC18 & LEU2 gene & yeast 2 micron
RC   YEp353, YEp354, YEp355 from pUC8 & URA3 gene & yeast 2 micron
RC   YEp356, YEp357, YEp358 from pUC18 & URA3 gene & yeast 2 micron
RC   YEp356R, YEp357R, YEp358R from pUC18 & URA3 gene & yeast 2 micron
RC   pHZ18 from pBR322 & URA3 gene & CYC1-rp51-lacZ & yeast 2 micron
RC   pJT24 from pBR322 & URA3 gene & GAL10 UAS & yeast 2 micron
RC   pSEY303 from pBR322 & URA3 gene & SUC2 gene & yeast 2 micron
RC   pGTyH3 from pBR322 & URA3 gene & GAL1-Ty & yeast 2 micron
RC   pLB318 from pBR322 & TRP1 gene & FLP gene & ARS1 & yeast 2 micron
RC   YEp51-FLP from pBR322 & LEU2 gene & FLP gene & yeast 2 micron
RC   pFV17 from pBR322 & LEU2 gene & yeast 2 micron
RC   pGAL4 from pBR322 & URA3 gene & GAL4 gene & yeast 2 micron
RC   p82-6B-LEU from pMB9 & LEU2 gene & yeast 2 micron
RC   pPS13 from pIC19R & URA3 gene & yeast 2 micron
RC   YEpHO from YEp13 & HO endonuclease gene
RC   pGALHO from YEp51 & HO endonuclease gene
RC   pMR848 from pSC101 & URA3 gene & yeast 2 micron
RC   p2-1A from pYcDE8 & Schwanniomyces occidentalis ODC gene
RC   pADE from pYcDE8 & Schwanniomyces occidentalis ADE2 gene
RC   pAR38 from YEp51
RC   UTX25 from YEp13
RC   UTX45 from YEp13
RC   UTX46 from YEp13
RC   pYT760.ADH1 from pYT760 & ADH1 gene
RC   pLG669-Z from YEp24 & CYC1 promoter & lacZ gene
RC   pLGSD5 from pLG669-Z & CYC1 UAS
RC   pRY121 from pLG669-Z & GAL1 promoter
RC   pRY131 from pLG669-Z & GAL1 promoter
RC   pIT210 from pBR322 & LEU2 gene & HSP1-lacZ gene
RC   pJH5 from pUC8 & LEU2 gene & URA3 gene & CEN4 or pNN130
RA   Rose A.B., Broach J.R.;
RT   "Propagation and expression of cloned genes in yeast: 2-microns
RT   circle-based vectors";
RL   Meth. Enzymol. 185:234-279(1990).
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CC   YE-type shuttle vector permitting FLP-driven high copy number.
CC   (personal communication)
CC   In S. cerevisiae, requires a host expressing FLP such as YPH485 or
CC   YPH494, or a host modified by pFV17 (ATCC 77170). (personal
CC   communication)
CC   When propagated in a strain carrying an integrated copy of GAL10-FLP,
CC   the copy number is 10-20 without induction and 200-400 when induced by
CC   galactose.
CC   The order of the major features in this plasmid is:REP3 - D - REP1 -
CC   REP2 - TRP1 - MCS (NotI - BamHI/NotI) - kanR.
CC   Constructed by inserting a BalI/EcoRV fragment of the 2 micron circle
CC   (interrupting FLP) into the SmaI site of pHSS6 which had been
CC   modified by the addition of a TRP1 gene.
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--4.8,2.2,1.4, 1.2; BamHI--9.6; NotI--9.6; ClaI--9.6. (ATCC
CC   staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pIA2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae YPH485)(Saccharomyces cerevisiae YPH494)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHSS6 2320bp, ori/kan gene
FT                   2. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS1/TRP1 gene
FT                   -> pHSS6-TRP 3700bp
FT                   1. pHSS6-TRP 3700bp, ori/kan gene/TRP1
FT                   2. yeast 2 micron, ori
FT                   -> pIA2 9300bp"
FT   misc_binding    0..0
FT                   /note="MCS NotI-EcoRI-ClaI-HindIII-XbaI-BglII-PstI-
FT                   BamHI-NotI"
FT   misc_binding    0..0
FT                   /note="SIT unique NotI-ClaI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//