Return to this vector's summary.
ID PIA2 preliminary; circular DNA; SYN; 9300 BP.
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AC ATCC77160;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli plasmid vector pIA2 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pHSS6-URA from pHSS6 & URA3 gene
RC pHSS6-Trp from pHSS6 & TRP1 gene
RC pIA1 from pHSS6-URA & yeast 2 micron
RC pIA2 from pHSS6-Trp & yeast 2 micron
RC YEpADE8.1 from pUC18 & ADE8 gene & yeast 2 micron
RC pHV1 from pUC series & HIS3 & yeast 2 micron
RC pJM35 from pBR322 & lambda & yeast 2 micron & HIS3 gene
RC pYc1 from YEp6
RC pYF177 from yeast 2 micron & HIS3 gene & pBR322
RC pYF88 from pYF85 & HIS3 gene
RC pYF92 from pYF86 & HIS3 gene
RC YEp421 from YEp6 & YEp420
RC YEp430 from pRB328 & YEp420
RC YEp4-Sc2703 from YEp4 & HIS3 gene
RC pC500, pC501 from HIS4 gene & pJC75-58
RC pHKB52 from yeast 2 micron & LEU2 gene & pBR322
RC pJDB248 from yeast 2 micron & LEU2 gene & pMB9
RC pJH4 from yeast 2 micron & LEU2 gene & pUC8
RC pJZ1 from yeast 2 micron & LEU2 gene
RC pYF90 from yeast 2 micron & LEU2 gene & pBR322
RC pYF91 from yeast 2 micron & LEU2 gene & pBR322
RC pYK71 from YEp13 & T7 promoter phi10/terminator
RC pYK72 from YEp13 & T7 promoter phi10
RC pYT7810 from yeast 2 micron & LEU2 gene & pBR325
RC YEp13S or YEp213 from YEp13
RC pPL116 from YEp13
RC pPL160 from YEp13
RC pPL199, pPL200 from YEp13
RC YEp13M4 from yeast 2 micron & pUC19 & LEU2 gene
RC pMCB974 from YEp13 & pUC18
RC YEp423 from YEp21 & YEp420
RC YEpLEU2.1 from pUC119 & yeast 2 micron & LEU2 gene
RC YEpM7 from pUC19 & yeast 2 micron & LEU2 gene
RC YEp-DE from pUC18 & yeast 2 micron & LEU2 gene
RC pYTH4 from pBR322 & SUP4.0 & yeast 2 micron
RC pRC3 from pKC7 & TRP1 gene & ARS1
RC pRC4 from pRC3 & linker
RC pRE1052 from TRP1 gene & yeast 2 micron
RC pTV3 from pUC series & TRP1 gene & yeast 2 micron
RC YEp9T from pBR322 & TRP1 gene & yeast 2 micron
RC G18, G23 from pCR1 & URA3 gene & PTY39
RC pAB18 from YIp5 & yeast 2 micron A
RC pCOS40 from YIp5 & yeast 2 micron B
RC pG6 from pBR322 & yeast 2 micron B
RC pG63 from pG6 & URA3 gene
RC pG63-11 from pG63
RC pGN621 from YIp5 & yeast 2 micron B
RC pHCG3 from pHC79 & pG63-11
RC pJDB110 from pAT153 & URA3 gene & yeast 2 micron
RC pSEY8 from pBR322 & URA3 gene & yeast 2 micron
RC pTB199 from YIp5 & yeast 2 micron B
RC pTB220, pTB221 from pTB199
RC pTB224 from pTB220
RC pTG807 from pBR322 & URA3 gene & yeast 2 micron ori
RC pUV2 from pUC series & URA3 gene & yeast 2 micron
RC pYK2060 from YRp16 & pJDB219
RC YEp103 from YIp5 & yeast 2 micron
RC YEp16 from pBR322 & URA3 gene & yeast 2 micron
RC pGT38 from pBR322 & LEU2 & CEN3 & yeast 2 micron
RC pGT39 from pBR322 & LEU2 & CEN3 & yeast 2 micron
RC pScT6 from pBR322 & LEU2 gene & yeast 2 micron
RC YCp2micron from YEp13 & CEN5
RC pA3 from pBR322 & ADE3 gene & URA3 & yeast 2 micron
RC pABADE8 from pBR322 & ADE8 gene & LEU2
RC TLC-1 from YEp13 & CAN1 gene
RC pJM52 from pJM35 & LEU2 gene
RC YEp422 from YEp420 & YEp6
RC YEp431 from YEp420 & pRB328
RC YEp13a from YEp13
RC pRB28 from YEp21 & URA3 gene
RC pRB37, pRB38 from YEp21 & URA3 gene
RC YEp424 from YEp420 & YEp21
RC pDA6200 from YEp24 & LYS2 gene
RC YEp425 from YEp420 & pRB506
RC pYK2074, pYK2075 from pYK2060 & TRP1 gene
RC YEp428 from YEp420 & YRp7
RC YEp433 from YEp420 & pRB315
RC YEp24cry1 from YEp24 & CRY1 gene
RC pPL241 from pPL200 & DHFR gene
RC pJM81 from pJM52 & HSV tk gene
RC pJM94 from pJM35 & HSV tk gene
RC pADA1 from pAAH5 & DHFR gene
RC R5H from YEp24 & PBS2 gene
RC YEpPBS2 from YEp24 & PBS2 gene
RC pJR41 from YEp24 & tunicamycin resistance gene
RC YEp36 from YEp13 & copper MT gene
RC pUT302 from pEX-2 & Tn5, ble gene
RC pCH100 from pBR328 & pYcDE-2, ADC1 promoter
RC pYT11, pYT14 from pBR325 & yeast 2 micron
RC pYT11-LEU2 from pYT11 & LEU2 gene
RC pYT14-LEU2 from pYT14 & LEU2 gene
RC pLG88, pLG89 from pEX-2 & CYC1 promoter & hyg gene
RC pGH-1 from pLG89 & G418 gene & hyg gene
RC fdY5 from fd106 & kan gene
RC PTY39 from pCR1 & yeast 2 micron
RC pCVneo from pCV20 & Tn5, kan gene
RC pNF1 from YEp24 & kan gene
RC pNF2 from pNF1 & linker
RC pMC3G from pBR322 & URA3 gene & lacZ
RC pYE13G from pYE13 & kan gene
RC pRC1 from pKC7 & ARS1/TRP1 gene
RC pRC2 from YEp13 & pRC1
RC pRC3 from pRC2
RC pET13.1 from pBR322 & CUP1 gene
RC pMP81 from pCR1 & pJDB219
RC pC1/1 from pSI4
RC pJDB207 or pMA40 from pAT153 & pJDB219
RC pJDB209 from pAT153 & pJDB219
RC pJDB219 from pMB9 & yeast 2 micron
RC pL623 from pBR325 & LEU2 gene
RC pMH158 from pJRD158b & LEU2 gene
RC pMCB803 from pMH158
RC pMCB967 from pMH158
RC pMP78-1 from pBR325 & pJDB219
RC pMP80-3 from pBR325 & pJDB219
RC pMP80-4 from pBR325 & pJDB219
RC pPL262 from pC1/1 & pUC19
RC pPL269 from pC1/1 & pAT153
RC pPM40 from pBR322 & yeast 2 micron
RC pROG5 from pBR322 & pJDB219
RC pSI4 from pBR322 & pJDB219
RC pSI5 from pBR322 & pJDB219
RC pY43 from pMB1 & phage f1 ori & LEU2 gene
RC YEp437 from pSI4 & YEp6
RC pJDB210, pJDB211 from pAT153 & URA3
RC pKS2-20 from pJDB219 & YIp5
RC pYK2024 from pJDB219 & YRp16
RC YEp436 from pSI4 & YIp5
RC pAAH5 from YEp13 & ADC1 promoter
RC pACF301 from LEU2 gene & CYC1 terminator & ADC1 gene & linker
RC pAD1 from ADC1 promoter & pUC19 & LEU2 gene
RC pAD4 from pAD1 & ADC1 terminator
RC pAH5 from pAAH5 & FLP terminator
RC pAH9 from pAAH5 & FLP terminator
RC pAH10 from pAAH5 & FLP terminator
RC pAH21 from pAAH5 & FLP terminator
RC pAXalpha11 from ADC1 prom & MATalpha1 term & LEU2 gene & pBR322
RC pADH040-2 from pBR322 & ADC1 promoter
RC pADH0322-2 from pBR322 & ADC1 promoter
RC pMA56 from pBR322 & ADC1 prom & FLP term & TRP1 gene
RC pMAC561 from pBR322 & ADC1 prom & CYC1 term & TRP1 gene
RC pWY4 from pBR322 & ADC1 prom & ADC1 term & TRP1 gene
RC pYcDE8 from pBR322 & ADC1 prom & CYC1 term & TRP1 gene
RC pYcDE-2 from pBR322 & ADC1 prom & CYC1 term & TRP1 gene
RC pCH98 from pYcDE-2
RC pRIT10774 from pBR322 & ARG3 prom & ARG3 term & LEU2 gene
RC pRIT10779 from pBR322 & ARG3 prom & ARG3 term & LEU2 gene
RC pEX-2 from pBR322 & CYC1 prom & CYC1 term & URA3 gene
RC pEX-4 from pEX-2 & kan gene
RC pAC1 from pBR322 & ENO1 prom & ENO1 term & LEU2 gene
RC pMAC101 from pAC1
RC pYE8 from interferon gene & pBR322 & GAPDH prom & FLP term & TRP1
RC pGPD-1 from pBR322 & GPD promoter & TRP1 terminator & TRP1 gene
RC pGPD-2 from pBR322 & GPD promoter & PGK terminator & TRP1 gene
RC pMA230 from pBR322 & PGK promoter & LEU2 gene
RC pMA301 from pBR322 & PGK promoter & LEU2 gene
RC pMA91 from pMA301 & PGK terminator
RC YEpIPT from pBR322 & PGK promoter & FLP terminator & TRP1 gene
RC pMA36 from pBR322 & TRP1 promoter & LEU2 gene
RC YEp434 from pGM65 & YEp21
RC YEp435 from pGM65 & YEp21
RC pLG12 from pLG1/4delta60 & GAL4 gene
RC pLG1/4delta60 from pBR322 & GAL1 promoter & GAL4 terminator & leu2d
RC pYMS-3 from pBR322 & GAL1 promoter & ADC1 terminator & leu2d gene
RC YEpG3 from pMA91
RC pYRF101 from pLG1/4delta60 & URA3 gene
RC pYRF102 from pLG12 & URA3 gene
RC pCGE329 from pBR322 & GAL1 promoter & URA3 gene
RC pGAL-X/P from pBR322 & GAL1 promoter & URA3 gene
RC pMAC105 from pBR322 & GAL1-ENO1 promoter & ENO1 terminator & LEU2 gene
RC YEp213-G from YEp213 & GAL1-GAL10 promoter
RC pGM65 from YEp420 & GAL1-GAL10 promoter
RC YEp57 from pAR38 & HIS3 gene
RC YEp51 from pBR322 & GAL10 promoter & FLP terminator & LEU2 gene
RC YEp52 from YEp51
RC YEp55 from YEp51 & GAL10 ATG
RC YEp62 from pBR322 & GAL10 promoter & lacZ terminator & LEU2 gene
RC YEpBam from YEp51
RC YEpUC from YEp51 & pUC8
RC YEp61 from pBR322 & GAL10 promoter & lacZ terminator & leu2d gene
RC YEp56 from pAR38 & LYS2 gene
RC Yep54 from pAR38 & ARS1/TRP1 gene
RC YEp53 from pAR38 & URA3 gene
RC pEMBLyex2 from pEMBL series & pEX-2
RC pEMBLyex4 from pEMBL series & pEX-4
RC pEMBLyexT3 from pEMBLyex4 & T3 promoter
RC pEMBLyexT7 from pEMBLyex4 & T7 promoter
RC pAM81 from pAT77
RC pAM82 from pAT77
RC pAT77 from pBR322 & PHO5 promoter & LEU2 gene & ARS1
RC pJDB207/PHO5 from pJDB207 & PHO5 gene
RC pYE4 from pBR322 & PHO5 promoter & TRP1 terminator & TRP1 gene
RC pYE7 from pBR322 & PHO5 promoter & FLP terminator & TRP1 & interferon
RC p1A1R1-15 from p1A1 & TRP1 gene
RC pPV1 from pHCG3 & PHO5 promoter
RC pPV2 from pPV1
RC SB10-2 from YEp24 & PHO5 promoter
RC palphaADH2 from pBR322 & ADH2 prom & FLP term & TRP1 & MFalpha1 signal
RC YEpsec1 from pEMBLyex2 & oligo
RC pJC1-5 from pBR322 & MFalpha1 promoter/terminator/gene & LEU2 gene
RC pTG881 from pBR322 & MFalpha1 promoter/signal & PGK terminator & URA3
RC YEp70alphaT from pBR322 & MFalpha1 prom/signal & FLP term & TRP1
RC pMFalpha8 from pBR322 & MFalpha1 prom/signal & TRP5 term & TRP1 & ARS1
RC pSEY210 from pBR322 & MFalpha1 prom/signal & URA3 gene & SUC2
RC pTG1819 from pBR322 & PGK prom/term & MFalpha1 signal & URA3 & LEU2
RC pNW033 from pBR322 & PGK prom & pGKL1 & TRP1 term & TRP1 gene
RC p1A1 from YIp5 & PHO5 promoter/signal & yeast 2 micron
RC pCGS681 from pBR322 & TPI prom & SUC2 signal & URA3 gene
RC pIT213 from pBR322 & URA3 gene & yeast 2 micron
RC pEP100 from pBR327 & LEU2 gene & yeast 2 micron
RC pJM102 from pBR322 & LEU2 gene & HSV tk gene & yeast 2 micron
RC pMC1587 from pBR322 & LEU2 gene & yeast 2 micron
RC pYT760 from pBR322 & LEU2 gene & yeast 2 micron
RC pRB45 from pBR322 & LEU2 gene & URA3 gene & yeast 2 micron
RC pJRB10 from pBR322 & leu2d gene & yeast 2 micron
RC pSL24 from pBR322 & leu2d gene & yeast 2 micron
RC YEpZ36 from pBR322 & leu2d gene & yeast 2 micron
RC pMC2010 from pBR322 & TRP1 gene & yeast 2 micron
RC pCGS139 from pBR322 & URA3 gene & yeast 2 micron
RC pMC1585 from pBR322 & URA3 gene & yeast 2 micron
RC pRY140 from pBR322 & URA3 gene & yeast 2 micron
RC pSEY101 from pCGS139
RC pLG670-Z from pLG669-Z
RC YEp363, YEp364, YEp365 from pUC8 & LEU2 gene & yeast 2 micron
RC YEp363R, YEp364R, YEp365R from pUC8 & LEU2 gene & yeast 2 micron
RC YEp366, YEp367, YEp368 from pUC18 & LEU2 gene & yeast 2 micron
RC YEp353, YEp354, YEp355 from pUC8 & URA3 gene & yeast 2 micron
RC YEp356, YEp357, YEp358 from pUC18 & URA3 gene & yeast 2 micron
RC YEp356R, YEp357R, YEp358R from pUC18 & URA3 gene & yeast 2 micron
RC pHZ18 from pBR322 & URA3 gene & CYC1-rp51-lacZ & yeast 2 micron
RC pJT24 from pBR322 & URA3 gene & GAL10 UAS & yeast 2 micron
RC pSEY303 from pBR322 & URA3 gene & SUC2 gene & yeast 2 micron
RC pGTyH3 from pBR322 & URA3 gene & GAL1-Ty & yeast 2 micron
RC pLB318 from pBR322 & TRP1 gene & FLP gene & ARS1 & yeast 2 micron
RC YEp51-FLP from pBR322 & LEU2 gene & FLP gene & yeast 2 micron
RC pFV17 from pBR322 & LEU2 gene & yeast 2 micron
RC pGAL4 from pBR322 & URA3 gene & GAL4 gene & yeast 2 micron
RC p82-6B-LEU from pMB9 & LEU2 gene & yeast 2 micron
RC pPS13 from pIC19R & URA3 gene & yeast 2 micron
RC YEpHO from YEp13 & HO endonuclease gene
RC pGALHO from YEp51 & HO endonuclease gene
RC pMR848 from pSC101 & URA3 gene & yeast 2 micron
RC p2-1A from pYcDE8 & Schwanniomyces occidentalis ODC gene
RC pADE from pYcDE8 & Schwanniomyces occidentalis ADE2 gene
RC pAR38 from YEp51
RC UTX25 from YEp13
RC UTX45 from YEp13
RC UTX46 from YEp13
RC pYT760.ADH1 from pYT760 & ADH1 gene
RC pLG669-Z from YEp24 & CYC1 promoter & lacZ gene
RC pLGSD5 from pLG669-Z & CYC1 UAS
RC pRY121 from pLG669-Z & GAL1 promoter
RC pRY131 from pLG669-Z & GAL1 promoter
RC pIT210 from pBR322 & LEU2 gene & HSP1-lacZ gene
RC pJH5 from pUC8 & LEU2 gene & URA3 gene & CEN4 or pNN130
RA Rose A.B., Broach J.R.;
RT "Propagation and expression of cloned genes in yeast: 2-microns
RT circle-based vectors";
RL Meth. Enzymol. 185:234-279(1990).
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CC YE-type shuttle vector permitting FLP-driven high copy number.
CC (personal communication)
CC In S. cerevisiae, requires a host expressing FLP such as YPH485 or
CC YPH494, or a host modified by pFV17 (ATCC 77170). (personal
CC communication)
CC When propagated in a strain carrying an integrated copy of GAL10-FLP,
CC the copy number is 10-20 without induction and 200-400 when induced by
CC galactose.
CC The order of the major features in this plasmid is:REP3 - D - REP1 -
CC REP2 - TRP1 - MCS (NotI - BamHI/NotI) - kanR.
CC Constructed by inserting a BalI/EcoRV fragment of the 2 micron circle
CC (interrupting FLP) into the SmaI site of pHSS6 which had been
CC modified by the addition of a TRP1 gene.
CC Restriction digests of the clone give the following sizes (kb):
CC HindIII--4.8,2.2,1.4, 1.2; BamHI--9.6; NotI--9.6; ClaI--9.6. (ATCC
CC staff)
CC Medium is 1236 LB plus kanamycin.
CC NM (pIA2)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC HO (Saccharomyces cerevisiae YPH485)(Saccharomyces cerevisiae YPH494)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pHSS6 2320bp, ori/kan gene
FT 2. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS1/TRP1 gene
FT -> pHSS6-TRP 3700bp
FT 1. pHSS6-TRP 3700bp, ori/kan gene/TRP1
FT 2. yeast 2 micron, ori
FT -> pIA2 9300bp"
FT misc_binding 0..0
FT /note="MCS NotI-EcoRI-ClaI-HindIII-XbaI-BglII-PstI-
FT BamHI-NotI"
FT misc_binding 0..0
FT /note="SIT unique NotI-ClaI-BamHI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT CDS 0..0
FT /note="ANT E. coli kanamycin resistance gene (kan)"
FT CDS 0..0
FT /note="ANT yeast TRP1 gene"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
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