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ID   PIJ2925    preliminary; circular DNA; SYN; 95 BP.
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AC   X69147;
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DT   15-DEC-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIJ2925 - incomplete, lacZ-alpha.
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KW   cloning vector; alpha-complementation; modified lacZ-alpha.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-95
RC   pIJ2925
RA   Bibb M.J.;
RT   ;
RL   Submitted (09-NOV-1992) to the EMBL/GenBank/DDBJ databases by:
RL   Bibb M.J., John Innes Institute, John Innes Centre, Colney Lane,
RL   Norwich, NR4 7UH, UNITED KINGDOM.
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RN   [2]
RP   1-95
RC   pIJ2920 from pUC18 & linker
RC   pIJ2921, pIJ2922 from pIJ2920 & linker
RC   pIJ2925, pIJ2926 from pIJ2922 & pIJ486
RA   Janssen G.R., Bibb M.J.;
RT   "Derivatives of pUC18 that have BglII sites flanking a modified
RT   multiple cloning site and that retain the ability to identify
RT   recombinant clones by visual screening of Escherichia coli";
RL   Gene 124:133-134(1993).
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CC   NCBI gi: 58017
CC   NM (pIJ2925)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIJ702 5650bp
FT                   2. Tn5 PvuII-PvuII 1122bp 1426..2548, neo gene
FT                   -> pIJ627 6700bp
FT                   1. pIJ627 6700bp
FT                   2. phage fd Sau3A/MboI-Sau3A/MboI 332bp 1382..1714,
FT                   \ terminator [338bp]
FT                   -> pIJ424 7000bp
FT                   1. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pIJ445
FT                   1. pUC8 2665bp
FT                   -> pEC8 2600bp
FT                   1. pIJ424
FT                   2. pEC8
FT                   -> pIJ450
FT                   1. pIJ450
FT                   2. pIJ445, cat gene
FT                   -> pIJ462
FT                   1. pIJ462
FT                   -> pIJ477
FT                   1. pIJ477
FT                   2. pIJ462
FT                   -> pIJ486
FT                   1. pIJ2922 remove BglII-BglII, MCS/linker
FT                   \ 2700bp
FT                   2. pIJ486 PstI, center-proximal
FT                   cloning removed PstI
FT                   BglII-BglII, MCS
FT                   -> pIJ2925"
FT   CDS             6..>95
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   modified alpha peptide; NCBI gi: 58018"
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SQ   Sequence 95 BP; 22 A; 28 C; 24 G; 21 T; 0 other;
     atgacatgat taccagatct gcagccaagc ttgcatgccg gtcgactcta gaggatcccc
     gggtaccgag ctcgaattcc ccagatctga gcttg
//