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ID   PIJ487     preliminary; circular DNA; SYN; 1 BP.
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AC   IG5177;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIJ487 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pIJ629, pIJ627 from pIJ702 & Tn5, neo gene
RC   pIJ424 from pIJ627 & phage fd terminator
RC   pIJ425 from pIJ629 & phage fd terminator
RC   pIJ445 from cat gene
RC   pIJ451 from pIJ425 & pEC8
RC   pIJ450 from pIJ424 & pEC8
RC   [pIJ454 from pIJ series]
RC   pIJ463 from pIJ451 & pIJ445, cat gene
RC   pIJ462 from pIJ450 & pIJ445, cat gene
RC   pIJ478 from pIJ463
RC   pIJ485 from pIJ462
RC   pIJ477 from pIJ462
RC   pIJ486 from pIJ477 & pIJ462
RC   pIJ490 from pIJ486 & pIJ462
RC   pIJ487 from pIJ490
RC   pIJ466 from pIJ451 & pIJ454
RC   pIJ465 from pIJ450 & pIJ454
RC   pIJ480 from pIJ466
RC   pIJ479 from pIJ465
RA   Ward J.M., Janssen G.R., Kieser T., Bibb M.J., Buttner M.J.,
RA   Bibb M.J.;
RT   "Construction and characterization of a series of multi-copy promoter
RT   probe plasmid vectors for Streptomyces using the amino-glycoside
RT   phosphotransferase gene from Tn5 as indicator";
RL   Mol. Gen. Genet. 203:468-478(1986).
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RN   [2]
RC   pML100 from pIJ702 & pUC19
RC   pMX1 from pML100 & pBR322
RC   pMX11 from pMX1 & pUC19
RC   pMX100 from pMX11 & pIJ487
RA   Sugiyama M., Nomura H., Nimi O.;
RT   "Use of the tyrosinase gene from Streptomyces to probe promoter
RT   sequences for Escherichia coli";
RL   Plasmid 23:237-241(1990).
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CC   promoter. terminator. not original sequence.
CC   NM (pIJ487)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (pMX100 also from pIJ702 & pUC19 & pBR322)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIJ702 5650bp
FT                   2. Tn5 PvuII-PvuII 1122bp 1426..2548, neo gene
FT                   -> pIJ627 6700bp
FT                   1. pIJ627 6700bp
FT                   2. phage fd Sau3A/MboI-Sau3A/MboI 332bp 1382..1714,
FT                   \ terminator [338bp]
FT                   -> pIJ424 7000bp
FT                   1. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pIJ445
FT                   1. pUC8 2665bp
FT                   -> pEC8 2600bp
FT                   1. pIJ424
FT                   2. pEC8
FT                   -> pIJ450
FT                   1. pIJ450
FT                   2. pIJ445, cat gene
FT                   -> pIJ462
FT                   1. pIJ462
FT                   -> pIJ477
FT                   1. pIJ477
FT                   2. pIJ462
FT                   -> pIJ486
FT                   1. pIJ486
FT                   2. pIJ462
FT                   -> pIJ490
FT                   1. pIJ490
FT                   -> pIJ487"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//