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ID   PIJ702     preliminary; circular DNA; SYN; 5650 BP.
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AC   ATCC35287;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Streptomyces plasmid vector pIJ702 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pIJ680 from pIJ series]
RC   pIJ700 from pIJ37 & S.antibioticus tyrosinase gene
RC   pIJ701 from pIJ41 & S.antibioticus tyrosinase gene
RC   pIJ702, pIJ703, pIJ704, pIJ705 from pIJ350 & pIJ700
RA   Katz E., Thompson C.J., Hopwood D.A.;
RT   "Cloning and expression of the tyrosinase gene from Streptomyces
RT   antibioticus in Streptomyces lividans";
RL   J. Gen. Microbiol. 129:2703-2714(1983).
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RN   [2]
RC   pIJ385 from pIJ101 & tsr gene & aph gene
RC   pIJ922 from SCP2* & tsr gene
RA   Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J.,
RA   Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schempli H.;
RT   ;
RL   Genetic Manipulation of Streptomyces 0:0-0(1985).
RL   John Innes Foundation, Norwood.
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RN   [3]
RC   pIJ23 from SLP1.2 & S. erythreus mls gene
RC   pIJ28 from pBR322 & pIJ2
RC   pIJ30 from pBR322 & pIJ6, tsr gene
RC   pIJ31 from pBR322 & pIJ2, neo gene
RC   pIJ36 from pBR322 & pIJ13, vph gene
RC   pIJ37 from pIJ2
RC   pIJ41 from pIJ6 & pIJ37
RC   pIJ43 from pIJ23 & pBR322
RC   pIJ61 from pIJ41
RC   pIJ63 from pIJ1, aac gene
RA   Thompson C.J., Kieser T., Ward J.M., Hopwood D.A.;
RT   "Physical analysis of antibiotic-resistance genes from Streptomyces
RT   and their use in vector construction";
RL   Gene 20:51-62(1982).
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RN   [4]
RC   pIJ101 from Streptomyces lividans ISP5434
RC   pIJ102, pIJ103, pIJ104 from pIJ101
RC   pIJ24 from pIJ103 & pIJ2, aph gene
RC   pIJ36 from pBR322 & pIJ13, vph gene
RC   pIJ37 from pIJ2
RC   pIJ41 from pIJ6 & pIJ37
RC   pIJ58 from pIJ350 & pIJ2
RC   pIJ201 from pIJ101 & pIJ2, aph gene
RC   pIJ202 from pIJ101 & pIJ2, aph gene
RC   pIJ208 from pIJ101 & pIJ2, aph gene
RC   pIJ211 from pIJ101 & pIJ2, aph gene
RC   pIJ213 from pIJ101 & pIJ2, aph gene
RC   pIJ216 from pIJ101 & pIJ2, aph gene
RC   pIJ218 from pIJ101 & pIJ2, aph gene
RC   pIJ221 from pIJ101 & pIJ2, aph gene
RC   pIJ222 from pIJ101 & pIJ2, aph gene
RC   pIJ224 from pIJ101 & pIJ2, aph gene
RC   pIJ302 from pIJ101 & pIJ6, tsr gene
RC   pIJ303 from pIJ101 & pIJ6, tsr gene
RC   pIJ306 from pIJ101 & pIJ6, tsr gene
RC   pIJ307 from pIJ101 & pIJ6, tsr gene
RC   pIJ312 from pIJ101 & pIJ6, tsr gene
RC   pIJ319 from pIJ101 & pIJ6, tsr gene
RC   pIJ320 from pIJ101 & pIJ6, tsr gene
RC   pIJ324 from pIJ101 & pIJ6, tsr gene
RC   pIJ326 from pIJ101 & pIJ6, tsr gene
RC   pIJ328 from pIJ101 & pIJ6, tsr gene
RC   pIJ330 from pIJ101 & pIJ6, tsr gene
RC   pIJ333 from pIJ101 & pIJ6, tsr gene
RC   pIJ341 from pIJ101 & pIJ6, tsr gene
RC   pIJ345 from pIJ101 & pIJ6, tsr gene
RC   pIJ350 from pIJ102 & pIJ6, tsr gene
RC   pIJ351 from pIJ102 & pIJ6, tsr gene
RC   pIJ352 from pIJ102 & pIJ6, tsr gene
RC   pIJ353 from pIJ208 & pIJ13, vph gene
RC   pIJ354 from pIJ102 & pIJ13, vph gene
RC   pIJ355 from pIJ101 & pIJ13, vph gene
RC   pIJ356 from pIJ101 & pIJ13, vph gene
RC   pIJ360 from pIJ36 & pIJ350
RC   pIJ361 from pIJ351 & pIJ36
RC   pIJ363 from pIJ24
RC   pIJ364 from pIJ350 & pIJ36
RC   pIJ365 from pIJ350 & pIJ41
RC   pIJ366 from pIJ102 & pIJ13, vph gene
RC   pPZ4, pPZ5, pPZ12 from pIJ303
RC   from pACYC184 & pIJ355
RC   from pACYC184 & pIJ366
RA   Kieser T., Hopwood D.A., Wright H.M., Thompson C.J.;
RT   "pIJ101, a multi-copy broad host-range Streptomyces plasmid:
RT   functional analysis and development of DNA cloning vectors";
RL   Mol. Gen. Genet. 185:223-238(1982).
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RN   [5]
RC   pIJ4083 from pIJ487 & pAMB22, lambda t0 terminator/xylE gene
RC   pIJ2842, pIJ2843 from pXE4 & pAMB22, lambda t0 terminator/xylE gene
RA   Clayton T.M., Bibb M.J.;
RT   "Streptomyces promoter-probe plasmids that utilise the xylE gene
RT   of Pseudomonas putida";
RL   Nucleic Acids Res. 18:1077-1077(1990).
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RN   [6]
RC   pIJ629, pIJ627 from pIJ702 & Tn5, neo gene
RC   pIJ424 from pIJ627 & phage fd terminator
RC   pIJ425 from pIJ629 & phage fd terminator
RC   pIJ445 from cat gene
RC   pIJ451 from pIJ425 & pEC8
RC   pIJ450 from pIJ424 & pEC8
RC   [pIJ454 from pIJ series]
RC   pIJ463 from pIJ451 & pIJ445, cat gene
RC   pIJ462 from pIJ450 & pIJ445, cat gene
RC   pIJ478 from pIJ463
RC   pIJ485 from pIJ462
RC   pIJ477 from pIJ462
RC   pIJ486 from pIJ477 & pIJ462
RC   pIJ490 from pIJ486 & pIJ462
RC   pIJ487 from pIJ490
RC   pIJ466 from pIJ451 & pIJ454
RC   pIJ465 from pIJ450 & pIJ454
RC   pIJ480 from pIJ466
RC   pIJ479 from pIJ465
RA   Ward J.M., Janssen G.R., Kieser T., Bibb M.J., Buttner M.J.,
RA   Bibb M.J.;
RT   "Construction and characterization of a series of multi-copy promoter
RT   probe plasmid vectors for Streptomyces using the amino-glycoside
RT   phosphotransferase gene from Tn5 as indicator";
RL   Mol. Gen. Genet. 203:468-478(1986).
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RN   [7]
RC   pIJ361 from pIJ series
RA   Chater K.F., Hopwood D.A., Kieser T., Thompson C.J.;
RT   "Gene cloning in Streptomyces";
RL   Curr. Top. Microbiol. Immunol. 97:0-0(1982).
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RN   [8]
RC   SLP1 from Streptomyces
RC   SLP1.1, SLP1.2, SLP1.3, SLP1.4, SLP1.5, SLP1.6 from SLP1
RC   pIJ1 from SLP1.2 & S. fradiae aac gene
RC   pIJ2 from SLP1.2 & S. fradiae aph gene
RC   pIJ3 from SLP1.2 & S. fradiae aph gene
RC   pIJ4 from SLP1.2 & S. fradiae aac gene
RC   pIJ5 from SLP1.2 & S. azureus tsr gene
RC   pIJ6 from SLP1.2 & S. azureus tsr gene
RC   pIJ39 from S. lividans, tsr gene
RA   Thompson C.J., Ward J.M., Hopwood D.A.;
RT   "DNA cloning in Streptomyces: resistance genes from
RT   antibiotic-producing species";
RL   Nature 286:525-527(1980).
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RN   [9]
RC   pEC8 from pUC8
RC   pIJ642 from pIJ350
RA   Eckhardt T.;
RT   ;
RL   Unpublished (1986).
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RN   [10]
RC   pIJ13 from SLP1.2 & S. vinaceus vph gene
RC   pIJ21 from SLP1.2 & S. vinaceus vph gene
RA   Thompson C.J., Ward J.M., Hopwood D.A.;
RT   "Cloning of antibiotic resistance and nutritional genes in
RT   Streptomyces";
RL   J. Bacteriol. 151:668-677(1982).
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RN   [11]
RC   pMT660 from pIJ702
RA   Birch A.W., Cullum J.;
RT   "Temperature-sensitive mutants of the Streptomyces plasmid pIJ702";
RL   J. Gen. Microbiol. 131:1299-1303(1985).
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RN   [12]
RC   SCP2 from Streptomyces coelicolor
RC   SCP2* from Streptomyces coelicolor
RC   M110, M130, M109, M132, M124, M107, A700 from SCP2*
RA   Bibb M.J., Hopwood D.A.;
RT   "Genetic studies of the fertility plasmid SCP2 and its SCP2*
RT   variants in Streptomyces coelicolorA3(2)";
RL   J. Gen. Microbiol. 126:427-442(1981).
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RN   [13]
RC   pIJ922 from SCP2*
RA   Lydiate D.J., Malpartida F., Hopwood D.A.;
RT   "The Streptomyces plasmid SCP2*: its functional analysis and
RT   development into useful cloning vectors";
RL   Gene 35:223-235(1985).
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RN   [14]
RC   pIJ32 from pBR322 & S.fradiae aph gene
RA   Thompson C.J., Gray G.S.;
RT   "Nucleotide sequence of a streptomycete aminoglycoside
RT   phsophotransferase and its relationship to phsophotransferases
RT   encoded by resistance plasmids";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:5190-5194(1983).
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RN   [15]
RC   pIJ110 from S.parvulus & S.lividans
RC   pIJ408 from S.glaucescens & S.lividans
RC   pIJ410 from pIJ408 & vph gene
RC   pIJ409 from pIJ410 & pBR325
RC   pIJ111 from pIJ110 & tsr gene
RC   pIJ114, pIJ115 from pIJ110 & pBR327
RA   Hopwood D.A., Hintermann G., Kieser T., Wright H.M.;
RT   "Integrated DNA sequences in three streptomycetes form related
RT   autonomous plasmids after transfer to Streptomyces lividans";
RL   Plasmid 11:1-16(1984).
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RN   [16]
RC   pXE4 from SCP2*
RA   Ingram C., Brawner M., Youngman P., Westpheling J.;
RT   "xylE functions as an efficient reporter gene in Streptomyces
RT   spp.: use for the study of galP1, a catabolite-controlled
RT   promoter";
RL   J. Bacteriol. 171:6617-6624(1989).
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RN   [17]
RC   pAMB22 from xylE gene & lambda, t0 terminator
RC   pTG402 from xyl gene
RA   Zukowski M.M., Gaffney D.F., Speck D., Kauffman M., Findeli A.,
RA   Wiseup A., Lococq J.P.;
RT   "Chromogenic identification of genetic regulatory signals in
RT   Bacillus subtilis based on expression of a cloned Pseudomonas
RT   gene";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:1101-1105(1983).
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RN   [18]
RC   SRP1 from S.rimosus
RA   Friend E.J., Warren M., Hopwood D.A.;
RT   "Genetic evidence for a plasmid controlling fertility in an
RT   industrial strain of Streptomyces rimosus";
RL   J. Gen. Microbiol. 106:201-206(1978).
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CC   The tyrosinase (mel) gene of S. antibioticus was inserted at a BclI
CC   site of pIJ350, a non-conjugative broad host range vector.
CC   Tyrosinase is secreted during growth of S. antibioticus but remains
CC   intracellular in S. lividans.  Inserts at BglII, SphI or SstI sites
CC   inactivate the tyrosinase gene.
CC   Cloning vector with thiostrepton resistance (tsrR) and tyrosinase
CC   production (mel+) markers, for use in Streptomyces lividans.
CC   Medium is 1339 R2 YE medium.
CC   NM (pIJ702)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Streptomyces lividans 3131)(Streptomyces lividans)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Streptomyces coelicolor
FT                   -> SLP1
FT                   1. SLP1
FT                   -> SLP1.2 14400bp [PstI, BamHI in 2830bp region]
FT                   1. SLP1.2 BamHI
FT                   2. S. azureus MboI-MboI 6900bp, tsr gene
FT                   -> pIJ6 21300bp
FT                   1. Streptomyces lividans ISP5434
FT                   -> pIJ101 8900bp
FT                   1. pIJ101 remove 4700bp, no resistance genes
FT                   -> pIJ102 4200bp
FT                   1. pIJ102 remove small BclI-BclI
FT                   2. pIJ6 BclI-BclI 1100bp, tsr gene
FT                   -> pIJ350
FT                   1. SLP1.2 BamHI
FT                   2. S. fradiae MboI-MboI 13000bp, neo gene
FT                   -> pIJ2
FT                   1. pIJ2
FT                   -> pIJ37 14800bp [unique BclI]
FT                   1. pIJ37 BclI
FT                   2. S.antibioticus BclI-BclI 1550bp, tyrosinase gene
FT                   -> pIJ700
FT                   1. pIJ350 BclI
FT                   2. pIJ700 BclI-BclI 1550bp, tyrosinase gene
FT                   -> pIJ702"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   CDS             0..0
FT                   /note="ANT E. coli tsrR gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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