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ID   PINIA1     preliminary; circular DNA; SYN; 4900 BP.
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AC   M24182; V00302; J01645;
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DT   15-SEP-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIN-I-A1 or pKEN037 - incomplete, promoter.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-62
RC   pKEN002 from pBR322 & lpp gene
RC   pKEN005 from pBR322
RC   pKEN008 from pKEN005 & lpp gene
RC   pKEN013 from pBR322 & lpp gene
RC   pKEN033 from pKEN111 & pBR322
RC   pIN-I-A1 or pKEN037 from pKEN033
RC   pIN-I-A2 or pKEN039, pIN-I-A3 or pKEN040 from pKEN111 & pBR322
RC   pIN-II-A1 or pKEN045 from pKEN037 & pOP203-3
RC   pIN-II-A2 from pIN-I-A2 or pKEN039 & pOP203-3
RC   pIN-II-A3 from pKEN040 & pOP203-3
RC   pYM141 from pIN-II series
RA   Nakamura K., Inouye M.;
RT   "Construction of versatile expression cloning vehicles using the
RT   lipoprotein gene of Escherichia coli";
RL   EMBO J. 1:771-775(1982).
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RN   [2]
RC   pKEN211, pKEN212 from pSC101 & lambda lppSm-1, lpp gene
RC   pKEN111, pKEN112 from pSC101 & lambda lppEc-1, lpp gene
RC   pKEN113 from pKEN111
RA   Lee N., Nakamura K., Inouye M.;
RT   "Expression of the Serratia marcescens lipoproteins gene in
RT   Escherichia coli";
RL   J. Bacteriol. 146:861-866(1981).
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RN   [3]
RC   lambda lppSm-1 from Charon 14 & Serratia marcescens lpp gene
RC   pKEN221 from pBR322 & lambda lppSm-1
RA   Nakamura K., Inouye M.;
RT   "DNA sequence of the Serratia marcescens lipoprotein gene";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:1369-1373(1980).
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RN   [4]
RC   lambda lppEc-1 from lambda 540 & E.coli lpp gene
RA   Nakamura K., Inouye M.;
RT   "DNA sequence of the gene for the outer membrane lipoprotein of
RT   E. coli: an extremely AT-rich promoter";
RL   Cell 18:1109-1117(1979).
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CC   NCBI gi: 209027
CC   NM (pIN-I-A1)(pKEN037)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(constitutive expression)
CC   SE ()
CC   PA (pKEN033 from pKEN111 and pBR322)
CC   BR ()
CC   OF (pIN-I-A2 or pKEN039)(pIN-I-A3 or pKEN040)
CC   OF (pIN-II-A1 or pKEN045 from pKEN037 and pOP203-3)
CC   OF (pIN-II-A2 from pKEN039 & pOP203-3)
CC   OF (pIN-II-A3 from pKEN040 & pOP203-3)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 540
FT                   2. E. coli HindIII-HindIII 10000bp, lpp gene
FT                   \ #V00302/#J01645
FT                   -> lambda lppEc-1
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. lambda lppEc-1 HaeIII-HaeIII 2800bp, lpp gene
FT                   \ #V00302/#J01645
FT                   EcoRI linker 8bp ggaattcc:EcoRI linker 8bp ggaattcc
FT                   -> pKEN111 12000bp
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   S1 nuclease
FT                   2. pKEN111 AluI-AluI 462bp, lpp gene -417 to +45
FT                   \ #V00302/#J01645
FT                   -> pKEN002 4820bp [HindIII at 5' lpp]
FT                   1. pBR322 remove EcoRI-HindIII 31bp 4360..4361..30,
FT                   \ tet prom/4332bp [29bp]
FT                   -> pKEN005 4332bp [EcoRI]
FT                   1. pKEN005 EcoRI 4332bp, pBR322 4360
FT                   2. pKEN111 AluI-AluI 462bp, lpp gene -417 to +45
FT                   \ #V00302/#J01645
FT                   EcoRI linker 8bp ggaattcc:EcoRI linker 8bp ggaattcc
FT                   -> pKEN008 4810bp
FT                   1. pKEN002 small PvuI-XbaI
FT                   2. pKEN008 small XbaI-EcoRI
FT                   3. pKEN005 large EcoRI-PvuI 3740bp,
FT                   \ pBR322 4360..4361..3739
FT                   -> pKEN010 [unique EcoRI]
FT                   1. pKEN010 remove HindIII-ClaI 207bp, before lpp gene
FT                   \ #V00302/#J01645
FT                   -> pKEN028
FT                   1. pBR322 remove HindIII-BamHI 346bp 30..376, 4015bp
FT                   -> pKEN014 4015bp
FT                   1. pKEN111 EcoRI-EcoRI 2800bp, lpp gene
FT                   \ #V00302/#J01645
FT                   PvuII, at +169
FT                   BamHI linker 10bp ccggatccgg:BamHI linker 10bp
FT                   \ ccggatccgg
FT                   HaeIII-HaeIII 2800bp, lpp gene
FT                   \ #V00302/#J01645
FT                   HpaI, at +1120
FT                   SalI linker 8bp ggtcgacc:SalI linker 8bp ggtcgacc
FT                   BamHI-SalI 2800bp
FT                   2. pKEN014 remove small BamHI-SalI, 2100bp
FT                   -> pKEN018 4900bp
FT                   1. pKEN018 remove small PvuI-EcoRI, 4900bp
FT                   2. pKEN028 small PvuI-EcoRI
FT                   -> pKEN030 4900bp
FT                   1. pKEN030 HindIII 4900bp
FT                   S1 nuclease
FT                   -> pKEN033 4900bp
FT                   1. pKEN033 EcoRI 4900bp
FT                   T4 DNA polymerase
FT                   HindIII linker 10bp ccaagcttgg
FT                   BamHI 4900bp
FT                   T4 DNA polymerase
FT                   HindIII linker 10bp ccaagcttgg
FT                   HindIII-HindIII 4900bp
FT                   -> pIN-I-A1 4900bp [unique EcoRI, HindIII, BamHI]"
FT   CDS             19..>45
FT                   /note="GEN E. coli prolipoprotein gene;
FT                   NCBI gi: 209028"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 62 BP; 20 A; 10 C; 18 G; 14 T; 0 other;
     atctagaggg tattaataat gaaagggaat tccaagcttg gatccggctg agcaacgacg
     tg
//