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ID   PINIA3     preliminary; circular DNA; SYN; 64 BP.
AC   M24184;
DT   15-SEP-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pIN-I-A3 - incomplete, promoter.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-64
RC   pKEN002 from pBR322 & lpp gene
RC   pKEN013 from pBR322 & lpp gene
RC   pKEN033 from pKEN111 & pBR322
RC   pIN-I-A1 or pKEN037 from pKEN033
RC   pIN-I-A2 or pKEN039, pIN-I-A3 or pKEN040 from pKEN111 & pBR322
RC   pIN-II-A1 or pKEN045 from pKEN037 & pOP203-3
RC   pIN-II-A2 from pIN-I-A2 or pKEN039 & pOP203-3
RC   pIN-II-A3 from pKEN040 & pOP203-3
RC   pYM141 from pIN-II series
RA   Nakamura K., Inouye M.;
RT   "Construction of versatile expression cloning vehicles using the
RT   lipoprotein gene of Escherichia coli";
RL   EMBO J. 1:771-775(1982).
CC   NCBI gi: 209031
CC   NM (pIN-I-A3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(constitutive expression)
CC   SE ()
CC   PA (pKEN037)
CC   BR (pIN-I-A2)
CC   OF (pIN-II-A3)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda 540
FT                   2. E. coli HindIII-HindIII 10000bp, lpp gene
FT                   \ #V00302/#J01645
FT                   -> lambda lppEc-1
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. lambda lppEc-1 HaeIII-HaeIII 2800bp, lpp gene
FT                   \ #V00302/#J01645
FT                   EcoRI linker 8bp ggaattcc:EcoRI linker 8bp ggaattcc
FT                   -> pKEN111 12000bp
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   S1 nuclease
FT                   2. pKEN111 AluI-AluI 462bp, lpp gene -417 to +45
FT                   \ #V00302/#J01645
FT                   -> pKEN002 4820bp [HindIII at 5' lpp]
FT                   1. pBR322 remove EcoRI-HindIII 31bp 4360..4361..30,
FT                   \ tet prom/4332bp [29bp]
FT                   -> pKEN005 4332bp [EcoRI]
FT                   1. pKEN005 EcoRI 4332bp, pBR322 4360
FT                   2. pKEN111 AluI-AluI 462bp, lpp gene -417 to +45
FT                   \ #V00302/#J01645
FT                   EcoRI linker 8bp ggaattcc:EcoRI linker 8bp ggaattcc
FT                   -> pKEN008 4810bp
FT                   1. pKEN002 small PvuI-XbaI
FT                   2. pKEN008 small XbaI-EcoRI
FT                   3. pKEN005 large EcoRI-PvuI 3740bp,
FT                   \ pBR322 4360..4361..3739
FT                   -> pKEN010 [unique EcoRI]
FT                   1. pKEN010 remove HindIII-ClaI 207bp, before lpp gene
FT                   \ #V00302/#J01645
FT                   -> pKEN028
FT                   1. pBR322 remove HindIII-BamHI 346bp 30..376, 4015bp
FT                   -> pKEN014 4015bp
FT                   1. pKEN111 EcoRI-EcoRI 2800bp, lpp gene
FT                   \ #V00302/#J01645
FT                   PvuII, at +169
FT                   BamHI linker 10bp ccggatccgg:BamHI linker 10bp
FT                   \ ccggatccgg
FT                   HaeIII-HaeIII 2800bp, lpp gene
FT                   \ #V00302/#J01645
FT                   HpaI, at +1120
FT                   SalI linker 8bp ggtcgacc:SalI linker 8bp ggtcgacc
FT                   BamHI-SalI 2800bp
FT                   2. pKEN014 remove small BamHI-SalI, 2100bp
FT                   -> pKEN018 4900bp
FT                   1. pKEN018 remove small PvuI-EcoRI, 4900bp
FT                   2. pKEN028 small PvuI-EcoRI
FT                   -> pKEN030 4900bp
FT                   1. pKEN030 EcoRI
FT                   S1 nuclease
FT                   EcoRI linker 8bp ggaattcc
FT                   -> pKEN036
FT                   1. pIN-I-A1 remove small XbaI-EcoRI
FT                   2. pKEN036 small XbaI-EcoRI
FT                   -> pIN-I-A3 4900bp"
FT   CDS             19..>48
FT                   /note="GEN E. coli prolipoprotein gene;
FT                   NCBI gi: 209032"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 64 BP; 20 A; 10 C; 20 G; 14 T; 0 other;
     atctagaggg tattaataat gaaaggggga attccaagct tggatccggc tgagcaacga