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ID   PINIIIA1   preliminary; circular DNA; SYN; 7400 BP.
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AC   IG7016;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pINIII-A1 or pYM061 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pFB140 from lacI gene
RC   pYM051 from pBR322 & pFB140, lacI gene
RC   pYM053 from pYM111 & pYM051
RC   pYM058 from pYM053
RC   pINIII-A1 from pIN-II-A1 & pYM058
RC   [pYM151 from pBR322]
RC   pYM153 from pYM151
RC   pYM071 from pYM153 & pINIII-A1
RC   pINIII-A3 from pYM071
RC   pINIII-A2 from pINIII-A3 & pAT606
RC   pINIII-C1 from pINIII-A1
RC   pINIII-C2 from pINIII-A2
RC   pINIII-C3 from pINIII-A3
RA   Masui Y., Mizuno T., Inouye M.;
RT   "Novel high-level expression cloning vehicles: 10^4 fold
RT   amplification of Escherichia coli minor protein";
RL   Biotechnology 2:81-85(1984).
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RN   [2]
RC   pKEN114 from pKEN111 & pOP203-3
RC   pKEN115 from pKEN114 & pKEN002
RC   pKEN121, pKEN122 from pKEN114 & pKEN013
RC   pKEN124 from pKEN121
RC   pYM111, pYM112 from pKEN124 & linker
RC   pKEN125 from pYM111 & pYM112
RC   pKEN126 from pKEN124 & pKEN125
RA   Nakamura K., Masui Y., Inouye M.;
RT   "Use of a lac promoter-operator fragment as a transcriptional
RT   control switch for expression of the constitutive lpp gene in
RT   Escherichia coli";
RL   J. Mol. Appl. Genet. 1:289-299(1982).
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RN   [3]
RC   pKEN005 from lpp gene
RC   pKM005 from lpp gene
RC   pI-MIA from pKN402
RA   Masui Y., Coleman J., Inouye M.;
RT   "Multipurpose expression cloning vehicles in Escherichia coli";
RL   Experimental Manipulation of Gene Expression 0:15-32(1983).
RL   Inouye M., ed., Academic Press, New York.
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RN   [4]
RC   pAT224E from pAT039 & pAT224 & linker
RC   pAT505 from pAT427 & pEW007
RC   pAT707 from pAT427 & pIN-II-A1
RC   pAT707E from pAT707
RC   pAT606 from pAT707 & pIN-II-A1
RC   pAT606E from pAT606
RA   Mizuno T., Wurtzel E.T., Inouye M.;
RT   "Osmoregulation of gene expression. II. DNA sequences of the envZ
RT   gene of the ompB operon of Escherichia coli and characterization of
RT   its gene product";
RL   J. Biol. Chem. 257:13692-13698(1982).
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RN   [5]
RC   pAT039, pAT179, pAT224, pAT427 from pBR322 & ompB operon
RC   pEW007 from pBR322 & ompB operon
RA   Mizuno T., Wurtzel E.T., Inouye M.;
RT   "Cloning of the regulatory genes (ompR and envZ) for the matrix
RT   proteins of the Escherichia coli outer membrane";
RL   J. Bacteriol. 150:1462-1466(1982).
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CC   MCS, oligonucleotide linker.
CC   NM (pINIII-A1)(pYM061)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIN-II-A1)
CC   PA (pYM058 from pYM053 from pYM111/pYM051, from pBR322/pFB140)
CC   BR (pIN-III-A2)(pIN-III-A3)
CC   OF (pYM071)(pYM072 or PINIII-A3 from pYM071)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. E. coli, lac operon
FT                   -> pFB140
FT                   1. pBR322 remove EcoRI-PstI 748bp 3612..4360
FT                   2. pFB140 EcoRI-PstI, lacI gene
FT                   -> pYM051
FT                   1. pYM111 HpaI
FT                   BAP
FT                   2. pYM051 HincII(1)-HincII(3), lacI gene
FT                   -> pYM053
FT                   1. pYM053 HpaI-HpaI 789bp, lacI/lacZ
FT                   MspI, HincII-MspI of lacI gene 3'
FT                   S1 nuclease:S1 nuclease
FT                   2. pYM053 HpaI-HpaI, HincII-HincII of lacI/lacZ
FT                   BAP
FT                   -> pYM058
FT                   1. pIN-II-A1 SalI
FT                   BAP
FT                   2. pYM058 remove EcoRI-EcoRI 2400bp
FT                   S1 nuclease:S1 nuclease
FT                   -> pINIII-A1 7400bp"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO E. coli lpp gene promoter"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO E. coli lacPO operator"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
FT   CDS             0..0
FT                   /note="GEN E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT HincII/HpaI"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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