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ID   PINIIIA3   preliminary; circular DNA; SYN; 7400 BP.
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AC   IG7018;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pINIII-A3 or pYM072 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pFB140 from lacI gene
RC   pYM051 from pBR322 & pFB140, lacI gene
RC   pYM053 from pYM111 & pYM051
RC   pYM058 from pYM053
RC   pINIII-A1 from pIN-II-A1 & pYM058
RC   [pYM151 from pBR322]
RC   pYM153 from pYM151
RC   pYM071 from pYM153 & pINIII-A1
RC   pINIII-A3 from pYM071
RC   pINIII-A2 from pINIII-A3 & pAT606
RC   pINIII-C1 from pINIII-A1
RC   pINIII-C2 from pINIII-A2
RC   pINIII-C3 from pINIII-A3
RA   Masui Y., Mizuno T., Inouye M.;
RT   "Novel high-level expression cloning vehicles: 10^4 fold
RT   amplification of Escherichia coli minor protein";
RL   Biotechnology 2:81-85(1984).
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CC   MCS, oligonucleotide linker.
CC   NM (pINIII-A3)(pYM072)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIN-II-A1)(pYM071 from pYM153, from pYM151, and pYM061)
CC   BR (pIN-III-A2)(pIN-III-A1)
CC   OF (PINIII-A2 also from pAT606)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pYM111
FT                   2. pYM112
FT                   -> pKEN125
FT                   1. pKEN125 XbaI-XbaI 113bp, lacPO promoter
FT                   2. pYM151 XbaI
FT                   -> pYM153
FT                   1. pYM153 PstI-EcoRI 1300bp
FT                   2. pINIII-A1 EcoRI-PstI 6300bp
FT                   -> pYM071 7600bp
FT                   1. pYM071 remove small XbaI-XbaI 200bp
FT                   Klenow:Klenow
FT                   -> pINIII-A3 7400bp"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO E. coli lpp gene promoter"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO E. coli lacPO operator"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   CDS             0..0
FT                   /note="GEN E. coli lacI repressor gene"
FT   misc_feature    0..0
FT                   /note="E. coli lpp gene fragment"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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