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ID   PINIIIOMP2 preliminary; circular DNA; SYN; 7500 BP.
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AC   IG5037;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIN-III-ompA2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pINIII-A1, pINIII-A2, pINIII-A3 from pINIII series
RC   pINIII-B1, pINIII-B2, pINIII-B3 from pINIII series
RC   pINIII-C1, pINIII-C2, pINIII-C3 from pINIII series
RC   pINIII-Omp1, pINIII-Omp2, pINIII-Omp3 from pINIII series
RA   Lunn C.A., Takahara M., Inouye M.;
RT   "Secretion cloning vectors for guiding the localization of proteins
RT   in vivo";
RL   Curr. Top. Microbiol. Immunol. 125:59-74(1986).
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CC   MCS, oligonucleotide linker.
CC   NM (pIN-III-ompA2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIN-III from pIN from pBR322)
CC   BR (pIN-III-ompA1)(pIN-III-ompA3)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pINIII-A2 7400bp
FT                   2. E. coli ompA HhaI-HhaI 65bp, signal sequence
FT                   3. oligo, RBS
FT                   4. oligo, ompA signal peptide cleavage site
FT                   -> pIN-III-OmpA2 7500bp [unique EcoRI-HindIII-BamHI]"
FT   promoter        0..0
FT                   /note="PRO E. coli lpp gene & operator"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_feature    0..0
FT                   /note="ompA signal sequence"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
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SQ   Sequence 90 BP; 20 A; 23 C; 27 G; 20 T; 0 other;
     atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag
     gccgctgaat tccaagcttg gatccggctg
//