back Return to this vector's summary.
ID   PIP1088    preliminary; circular DNA; SYN; 251 BP.
AC   K01187; K01188;
DT   01-AUG-1984 (Rel. 1, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pIP1088 - incomplete, lt/rt transposable IS15.
KW   cloning vector; insertion element; plasmid; IS15; pBR322.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-71, left
RP   1-71, right
RC   pIP1087 from pBR322 & Tn1525, IS15
RC   pIP1088 from pIP1087
RA   Trieu-Cuot P., Labigne-Roussel A., Courvalin P.;
RT   "An IS15 insertion generates an eight-base-pair duplication of the
RT   target DNA";
RL   Gene 24:125-129(1983).
RN   [2]
RC   pIP1433 from Campylobacter coli, cat gene
RC   pAT95 from pIP1433 & pBR322
RC   pAT96 from pAT95 & pC194
RA   Trieu-Cuot P., Gerbaud G., Lambert T., Courvalin P.;
RT   "In vivo transfer of genetic information between Gram-positive and
RT   Gram-negative bacteria";
RL   EMBO J. 4:3583-3587(1985).
RN   [3]
RC   pIP1455 from Campylobacter coli, ori
RA   Lambert T., Gerbaud G., Trieu-Cuot P., Courvalin P.;
RT   "Structural relationship between the genes encoding 3'-aminoglycoside
RT   phosphotransferases in Campylobacter and in gram-positive cocci";
RL   Ann. Inst. Pasteur Microbiol. 136B:134-150(1985).
CC   Insert transposable module IS15 into nucleotide position 1430 of
CC   pBR322.
CC   Integration is accompanied by duplication of an 8 bp ('tctcgggc')
CC   target DNA sequence.
CC   NCBI gi: 207744 & 207745
CC   NM (pIP1088)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(IS15)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 1430..1430
FT                   2. Tn1525 4440bp, IS15/kan gene/IS15
FT                   -> pIP1087 [BglI at right end of Tn1525]
FT                   1. pIP1087 remove IS15/kan gene
FT                   recombination between IS15's
FT                   -> pIP1088"
FT   misc_recomb     41..42
FT                   /note="plasmid pBR322/transposable module IS15; left"
FT   misc_recomb     33..34
FT                   /note="transposable module IS15/plasmid pBR322; right"
SQ   Sequence 251 BP; 20 A; 45 C; 50 G; 27 T; 109 other;
     cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cggcactgtt gcaaagttag
     cgatgaggca gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     ggttgatcgc caccggctgt ttgcgacagt gcctctcggg cagcgttggg tcctggccac
     gggtgcgcat g