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ID   PITH       preliminary; circular DNA; SYN; 8600 BP.
AC   M17304; M17305;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pITH - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pITH from pIT213 & pChTrp24
RC   pH1S, pH1B from lambda & pITH
RA   Turgeon B.G., Garber R.C., Yoder O.C.;
RT   "Development of a fungal transformation system based on selection
RT   of sequences with promoter activity";
RL   Mol. Cell. Biol. 7:3297-3305(1987).
RN   [2]
RC   pIT122 from pBR322 & pKC222, hyg gene
RC   pIT123 from pIT122 & pKC222
RC   pYG100PR1 from pBR322 & pYG100
RC   pIT115 from pYG100PR1 & linker
RC   pIT116 from pIT115 & pINIII-A1
RC   pIT120 from pIT116 & pBH1
RC   pIT107 from pBR322 & pYG101H1
RC   pIT110 from pIT107 & linker
RC   pIT118 from pIT110 & pYG100BH
RC   pIT141 from pBR322 & PGK gene
RC   pIT143 from pUC8 & pIT141 & linker
RC   pNG614 from pNG18
RC   plasmid from pNG614 & YEp24
RC   pIT213 from pIT123 & YEp24
RA   Kaster K.R., Burgett S.G., Ingolia T.D.;
RT   "Hygromycin B resistance as dominant selectable marker in yeast";
RL   Curr. Genet. 8:353-358(1984).
RN   [3]
RC   pChTrp24 from YEp24 & C.heterostrophus DNA
RC   lambda ChTrp1- series from EMBL4 & C.heterostrophus DNA
RC   from pChTrp24
RC   pNC2 from YEp24 & N.crassa DNA
RC   pHY201 from YEp24 & A.nidulans DNA
RC   from pSP64 & pChTrp24
RC   pKBY2 from A.nidulans trpC gene
RC   from pSP64 & pKBY2
RC   from pSP65 & pKBY2
RA   Turgeon B.G., Macrae W.D., Garber R.C., Fink G.R., Yoder O.C.;
RT   "A cloned tryptophan-synthesis gene from the ascomycete Cochliobolus
RT   heterostrophus functions in Escherichia coli, yeast and
RT   Aspergillus nidulans";
RL   Gene 42:79-88(1986).
RN   [4]
RC   pB8 from pBR322 & Drosophila hsp70 gene
RC   lambda 100, lambda 101 from Charon 28 & S.cerevisiae S288C DNA
RC   pYG100 from lambda 100 & pBR322
RC   pYG100BH from pYG100
RC   YG101 from lambda 101 & pBR322
RA   Ingolia T.D., Slater M.R., Craig E.A.;
RT   "Saccharomyces cerevisiae contains a complex multigene family
RT   related to the major heat shock-inducible gene of Drosophila";
RL   Mol. Cell. Biol. 2:1388-1398(1982).
RN   [5]
RC   pYG101H1 from pBR322 & pYG100
RA   Slater M.R., Craig E.A.;
RT   ;
RL   Unpublished (1984).
RN   [6]
RC   pHS4, pHS12 from pBR322 & Drosophila hsp70 gene
RC   pG3, pG5 from pBR322 & Drosophila hsp70 gene
RC   pB8, pBH1, pBH15 from pBR322 & Drosophila hsp70 gene
RA   Craig E.A., McCarthy B.J., Wadsworth S.;
RT   "Sequence organization of two recombinant plasmids containing
RT   genes for the major heat shock-induced protein of D. melanogaster";
RL   Cell 16:575-588(1979).
CC   in GenBank. promoter
CC   NM (pITH)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pChTrp24 from YEp24)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pKC7 5835bp
FT                   2. pKC203 SacI-BglII 1547bp, hyg B gene
FT                   -> pKC222 7300bp
FT                   1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, 3984bp
FT                   2. pKC222 PstI-HphI 1500bp, hyg gene
FT                   \ pKC7 PstI =  523 1446 5086/HphI ?
FT                   :Klenow
FT                   :BamHI linker 8bp tggatcca
FT                   EcoRI-BamHI 1500bp, hyg gene
FT                   -> pIT122 5500bp
FT                   1. pIT122 EcoRI 5500bp
FT                   2. pKC222 EcoRI-EcoRI 1500bp, hyg gene
FT                   -> pIT123 7000bp
FT                   1. pIT123 BamHI-BglII 1500bp, hyg gene
FT                   2. YEp24 BamHI 7769bp 3785..3785
FT                   -> pIT213 9300bp
FT                   1. YEp24 7769bp
FT                   2. C.heterostrophus DNA 4700bp, TRP1 gene
FT                   -> pChTrp24 15800bp
FT                   1. pIT213 SalI 7000bp, pBR322 652..652
FT                   2. pChTrp24 AvaI-AvaI 3500bp, C.heterostrophus TRP1
FT                   -> plasmid 10500bp
FT                   1. plasmid remove HindIII-HindIII 1900bp, 8600bp
FT                   -> pITH 8600bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli hygB gene"
FT   CDS             0..0
FT                   /note="ANT C.heterostrophus TRP1 gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;