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ID   PJ5OMEGA   preliminary; circular DNA; SYN; 3700 BP.
AC   ATCC37721;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pJ5omega - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJ3omega from pBR322 & SV40 small t intron/polyA & SV40 early prom
RC   pJ4omega from pBR322 & SV40 small t intron/polyA & MMLV LTR
RC   pJ5omega from pBR322 & SV40 small t intron/polyA & MMTV LTR
RC   pJ5Eomega from pBR322 & SV40 small t intron/polyA & MSV enhan/MMTV LTR
RC   pJ6omega from pBR322 & SV40 small t intron/polyA & rat beta-actin prom
RC   pJ7omega from pBR322 & SV40 small t intron/polyA & SCMV IE94 prom
RA   Morgenstern J.P., Land H.;
RT   "A series of mammalian expression vectors and characterisation of
RT   their expression of a reporter gene in stably and transiently
RT   transfected cells";
RL   Nucleic Acids Res. 18:1068-1068(1990).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7; PvuI--2.2, 1.5.
CC   (ATCC staff)
CC   Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t
CC   antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and
CC   MMTV LTR (-360 to +150, glucocorticoid regulatory sequences, cap
CC   site). (personal communication)
CC   Demonstrates expression inducible by dexamethasone.
CC   A mammalian shuttle vector for expression of a sequence cloned into
CC   the polylinker site under the control of the MMTV LTR promoter. (ATCC
CC   staff)
CC   Medium is 1315 1065 plus ampicillin (100 ug/ul).
CC   NM (pJ5omega)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(E.coli DH5)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. SV40, small t intron/polyA
FT                   3. linker
FT                   \ HindIII-SalI-XbaI-BamHI-SstI-EcoRI-ClaI-KpnI-BglII
FT                   4. MMTV, LTR
FT                   -> pJ5omega 3700bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PvuI-SalI-XbaI-BamHI-SmaI-SstI-
FT                   EcoRI-ClaI-KpnI-BglII"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PvuI-SalI-XbaI-BamHI-
FT                   SmaI-SstI-EcoRI-ClaI-KpnI-BglII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO MMTV LTR"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;