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ID   PJ6OMEGA   preliminary; circular DNA; SYN; 3500 BP.
AC   ATCC37723;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pJ6omega - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJ3omega from pBR322 & SV40 small t intron/polyA & SV40 early prom
RC   pJ4omega from pBR322 & SV40 small t intron/polyA & MMLV LTR
RC   pJ5omega from pBR322 & SV40 small t intron/polyA & MMTV LTR
RC   pJ5Eomega from pBR322 & SV40 small t intron/polyA & MSV enhan/MMTV LTR
RC   pJ6omega from pBR322 & SV40 small t intron/polyA & rat beta-actin prom
RC   pJ7omega from pBR322 & SV40 small t intron/polyA & SCMV IE94 prom
RA   Morgenstern J.P., Land H.;
RT   "A series of mammalian expression vectors and characterisation of
RT   their expression of a reporter gene in stably and transiently
RT   transfected cells";
RL   Nucleic Acids Res. 18:1068-1068(1990).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 1.35.
CC   (ATCC staff)
CC   The actin promoter has been shown to be expressed efficiently in
CC   undifferentiated cells in which transcription from other promoters
CC   (SV40 early, Mo MuLV LTR, MMTV LTR) is severely repressed.
CC   A mammalian expression vector for expression of a sequence cloned into
CD   the polylinker site under the control of the rat beta actin promoter.
CC   (ATCC staff)
CC   Medium is 1315 1065 plus ampicillin (100 ug/ul).
CC   NM (pJ6omega)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(E.coli DH5)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. SV40, small t intron/polyA
FT                   3. linker
FT                   \ HindIII-SalI-XbaI-BamHI-SstI-EcoRI-ClaI-KpnI-BglII
FT                   4. rat, beta-actin promoter
FT                   -> pJ6omega 3500bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PvuI-SalI-XbaI-BamHI-SmaI-SstI-
FT                   EcoRI-ClaI-KpnI-BglII"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PvuI-SalI-XbaI-BamHI-
FT                   SmaI-SstI-EcoRI-ClaI-KpnI-BglII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO rat beta-actin gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;