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ID   PJAJ7      preliminary; circular DNA; SYN; 11200 BP.
AC   ATCC37648;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli plasmid vector pJAJ7 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJAJ103 from pRK404
RC   pJAJ21 from pUC13 & pEC2.2, CMCase 2
RC   pJAJ7 from pJAJ103 & B870beta gene
RC   pJAJ9 from pJAJ103 & B870beta gene
RC   pJAJ17 from pJAJ7 & pEC1, CMCase 1
RC   pRW6 from pJAJ21 & pEC2.2, CMCase 2
RC   pREC1 from pJAJ7 & pEC1, CMCase 1
RC   pREC2.2 from pJAJ103 & pRW6
RC   pREC3 from pJAJ7 & pEC3, CMCase 3
RC   pRKEC2.2 from pRK404 & pEC2.2, CMCase 2
RA   Johnson J.A., Wong W.K., Beatty J.T.;
RT   "Expression of cellulase genes in Rhodobacter capsulatus by use of
RT   plasmid expression vectors";
RL   J. Bacteriol. 167:604-610(1986).
RN   [2]
RC   pRK291, pRK292 from pRK290 & linker
RC   pRK293 from pRK290 & pRK2501
RC   pRK310 from pRK290 & pUC9
RC   pRK311 from pRK310 & pHC79
RC   pGD499 from pMC279 & pRK291
RC   pGD500 from pGD499 & linker
RC   pGD926 from pMC931 & pRK657 & pRK291
RC   pRK252 from pRK248 & RK2
RC   pRK404 from pRK252 & pUC9
RA   Ditta G., Schmidhauser T., Yakobson E., Lu P., Liang X.W.,
RA   Finlay D.R., Guiney D., Helinski D.R.;
RT   "Plasmids related to the broad host range vector pRK290 useful for
RT   gene cloning and for monitoring gene expression";
RL   Plasmid 13:149-153(1985).
RN   [3]
RC   pDW1 from pBR322 & C.fimi DNA
RC   pEC1 from pBR322 & pDW1
RC   pEC2 from pBR322 & pDW1
RC   pEC3 from pBR322 & pDW1
RA   Gilkes N.R., Kilburn D.G., Lamgsford M.L., Miller R.C.,
RA   Wakarchuk W.W., Warren R.A., Whittle D.J., Wong W.K.;
RT   "Isolation and characterization of Escherichia coli clones
RT   expressing cellulase genes from Cellulomonas fimi";
RL   J. Gen. Microbiol. 130:1377-1384(1984).
RN   [4]
RC   pYCDE-2 from pBR322 & ADC1 gene & CYC1 gene & yeast 2 micron
RC   pEC2.2 from pBR322 & C.fimi CM-cellulase gene
RC   pOP from pYCDE-2 & pADH10A, yeast preprotoxin gene
RC   pN3 from pOP & linker
RC   plasmid from pEC2.2 & M13mp8
RC   pL5.19 from pOP & plasmid
RC   pK2.4 from pEC2.2 & pOP
RC   pK1.3 from pEC2.2 & pN3
RA   Skipper N., Sutherland M., Davies R.W., Kilburn D., Miller R.C.,
RA   Warren A., Wong R.;
RT   "Secretion of a bacterial cellulase by yeast";
RL   Science 230:958-960(1985).
RN   [5]
RC   pADH10A from yeast preprotoxin gene
RA   Lolle S., Skipper N., Bussey H., Thomas D.Y.;
RT   "The expression of cDNA clones of yeast M1 double-stranded RNA in
RT   yeast confers both killer and immunity phenotypes";
RL   EMBO J. 3:1383-1387(1984).
CC   Expression can be modulated as oxygen starvation of R. capsulatus
CC   stimulates transcription from the puf promoter.  Can be mobilized by
CC   conjugation from R. capsulatus into E.coli & other species that
CC   make up the host range of RK2.
CC   The XhoI/BamHI fragment of pJAJ21 containing the puf promoter and the
CC   5' end of the B870beta gene of Rhodobacter capsulatus was cloned into
CC   the BamHI site of the broad host range vector pJAJ103.
CC   A broad host range vector for the expression of heterologous genes in
CC   Rhodobacter capsulatus under the control of R. capsulatus puf
CC   promoter.
CC   pJAJ7 (ATCC 37648) & pJAJ9 (ATCC 37649) differ only in the
CC   orientation of the puf promoter.
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--13.0; SstI--13.0; BamHI--13.0. (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pJAJ7)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli JM83)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. RK2, oriT
FT                   -> pRK248
FT                   1. pRK248 remove small HaeII-HaeII
FT                   2. RK2 HaeII-HaeII 760bp, oriT
FT                   -> pRK252
FT                   1. pRK252 remove small HaeII-HaeII 760bp, RK2 oriT
FT                   2. pUC9 HaeII-HaeII 424bp 5..429, MCS/lacZalpha
FT                   -> pRK404
FT                   1. pRK404 remove PvuII-HindIII 200bp, lacZ promoter
FT                   -> pJAJ103 10000bp
FT                   1. pJAJ103 BamHI
FT                   2. C. fimi XhoI-BamHI 1200bp, rxcA prom/5' B870beta
FT                   -> pJAJ7 11200bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-SstI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   promoter        0..0
FT                   /note="PRO E. coli puf gene (rxcA)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;