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ID PJAJ7 preliminary; circular DNA; SYN; 11200 BP.
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AC ATCC37648;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Broad host range/E.coli plasmid vector pJAJ7 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pJAJ103 from pRK404
RC pJAJ21 from pUC13 & pEC2.2, CMCase 2
RC pJAJ7 from pJAJ103 & B870beta gene
RC pJAJ9 from pJAJ103 & B870beta gene
RC pJAJ17 from pJAJ7 & pEC1, CMCase 1
RC pRW6 from pJAJ21 & pEC2.2, CMCase 2
RC pREC1 from pJAJ7 & pEC1, CMCase 1
RC pREC2.2 from pJAJ103 & pRW6
RC pREC3 from pJAJ7 & pEC3, CMCase 3
RC pRKEC2.2 from pRK404 & pEC2.2, CMCase 2
RA Johnson J.A., Wong W.K., Beatty J.T.;
RT "Expression of cellulase genes in Rhodobacter capsulatus by use of
RT plasmid expression vectors";
RL J. Bacteriol. 167:604-610(1986).
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RN [2]
RC pRK291, pRK292 from pRK290 & linker
RC pRK293 from pRK290 & pRK2501
RC pRK310 from pRK290 & pUC9
RC pRK311 from pRK310 & pHC79
RC pGD499 from pMC279 & pRK291
RC pGD500 from pGD499 & linker
RC pGD926 from pMC931 & pRK657 & pRK291
RC pRK252 from pRK248 & RK2
RC pRK404 from pRK252 & pUC9
RA Ditta G., Schmidhauser T., Yakobson E., Lu P., Liang X.W.,
RA Finlay D.R., Guiney D., Helinski D.R.;
RT "Plasmids related to the broad host range vector pRK290 useful for
RT gene cloning and for monitoring gene expression";
RL Plasmid 13:149-153(1985).
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RN [3]
RC pDW1 from pBR322 & C.fimi DNA
RC pEC1 from pBR322 & pDW1
RC pEC2 from pBR322 & pDW1
RC pEC3 from pBR322 & pDW1
RA Gilkes N.R., Kilburn D.G., Lamgsford M.L., Miller R.C.,
RA Wakarchuk W.W., Warren R.A., Whittle D.J., Wong W.K.;
RT "Isolation and characterization of Escherichia coli clones
RT expressing cellulase genes from Cellulomonas fimi";
RL J. Gen. Microbiol. 130:1377-1384(1984).
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RN [4]
RC pYCDE-2 from pBR322 & ADC1 gene & CYC1 gene & yeast 2 micron
RC pEC2.2 from pBR322 & C.fimi CM-cellulase gene
RC pOP from pYCDE-2 & pADH10A, yeast preprotoxin gene
RC pN3 from pOP & linker
RC plasmid from pEC2.2 & M13mp8
RC pL5.19 from pOP & plasmid
RC pK2.4 from pEC2.2 & pOP
RC pK1.3 from pEC2.2 & pN3
RA Skipper N., Sutherland M., Davies R.W., Kilburn D., Miller R.C.,
RA Warren A., Wong R.;
RT "Secretion of a bacterial cellulase by yeast";
RL Science 230:958-960(1985).
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RN [5]
RC pADH10A from yeast preprotoxin gene
RA Lolle S., Skipper N., Bussey H., Thomas D.Y.;
RT "The expression of cDNA clones of yeast M1 double-stranded RNA in
RT yeast confers both killer and immunity phenotypes";
RL EMBO J. 3:1383-1387(1984).
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CC Expression can be modulated as oxygen starvation of R. capsulatus
CC stimulates transcription from the puf promoter. Can be mobilized by
CC conjugation from R. capsulatus into E.coli & other species that
CC make up the host range of RK2.
CC The XhoI/BamHI fragment of pJAJ21 containing the puf promoter and the
CC 5' end of the B870beta gene of Rhodobacter capsulatus was cloned into
CC the BamHI site of the broad host range vector pJAJ103.
CC A broad host range vector for the expression of heterologous genes in
CC Rhodobacter capsulatus under the control of R. capsulatus puf
CC promoter.
CC pJAJ7 (ATCC 37648) & pJAJ9 (ATCC 37649) differ only in the
CC orientation of the puf promoter.
CC Restriction digests of the clone give the following sizes (kb):
CC PstI--13.0; SstI--13.0; BamHI--13.0. (ATCC staff)
CC Medium is 1273 LB plus tetracycline.
CC NM (pJAJ7)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli JM83)(broad host range)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. RK2, oriT
FT -> pRK248
FT 1. pRK248 remove small HaeII-HaeII
FT 2. RK2 HaeII-HaeII 760bp, oriT
FT -> pRK252
FT 1. pRK252 remove small HaeII-HaeII 760bp, RK2 oriT
FT 2. pUC9 HaeII-HaeII 424bp 5..429, MCS/lacZalpha
FT -> pRK404
FT 1. pRK404 remove PvuII-HindIII 200bp, lacZ promoter
FT -> pJAJ103 10000bp
FT 1. pJAJ103 BamHI
FT 2. C. fimi XhoI-BamHI 1200bp, rxcA prom/5' B870beta
FT -> pJAJ7 11200bp"
FT misc_binding 0..0
FT /note="SIT unique PstI-SstI-BamHI"
FT rep_origin 0..0
FT /note="ORI E. coli RK2"
FT promoter 0..0
FT /note="PRO E. coli puf gene (rxcA)"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
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