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ID   PJAJ9      preliminary; circular DNA; SYN; 11200 BP.
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AC   ATCC37649;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli plasmid vector pJAJ9 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJAJ103 from pRK404
RC   pJAJ21 from pUC13 & pEC2.2, CMCase 2
RC   pJAJ7 from pJAJ103 & B870beta gene
RC   pJAJ9 from pJAJ103 & B870beta gene
RC   pJAJ17 from pJAJ7 & pEC1, CMCase 1
RC   pRW6 from pJAJ21 & pEC2.2, CMCase 2
RC   pREC1 from pJAJ7 & pEC1, CMCase 1
RC   pREC2.2 from pJAJ103 & pRW6
RC   pREC3 from pJAJ7 & pEC3, CMCase 3
RC   pRKEC2.2 from pRK404 & pEC2.2, CMCase 2
RA   Johnson J.A., Wong W.K., Beatty J.T.;
RT   "Expression of cellulase genes in Rhodobacter capsulatus by use of
RT   plasmid expression vectors";
RL   J. Bacteriol. 167:604-610(1986).
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CC   The XbaI/BamHI fragment of pJAJ21 containing the puf promoter and the
CC   5' end of the B870beta gene of R.capsulatus was cloned into the BamHI
CC   site of the broad host range vector pJAJ103.
CC   A broad host range vector for the expression of heterologous genes in
CC   Rhodobacter capsulatus under the control of R. capsulatus puf
CC   promoter.
CC   pJAJ7 (ATCC 37648) & pJAJ9 (ATCC 37649) differ only in the
CC   orientation of the puf promoter.
CC   Expression can be modulated as oxygen starvation of R. capsulatus
CC   stimulates transcription from the puf promoter.  Can be mobilized by
CC   conjugation from R. capsulatus into E.coli & other species that
CC   make up the host range of RK2.
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--13; SstI--13; BamHI--13. (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pJAJ9)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM83)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. RK2, oriT
FT                   -> pRK248
FT                   1. pRK248 remove small HaeII-HaeII
FT                   2. RK2 HaeII-HaeII 760bp, oriT
FT                   -> pRK252
FT                   1. pRK252 remove small HaeII-HaeII 760bp, RK2 oriT
FT                   2. pUC9 HaeII-HaeII 424bp 5..429, MCS/lacZalpha
FT                   -> pRK404
FT                   1. pRK404 remove PvuII-HindIII 200bp, lacZ promoter
FT                   -> pJAJ103 10000bp
FT                   1. pJAJ103 BamHI
FT                   2. C. fimi XhoI-BamHI 1200bp, rxcA prom/5' B870beta
FT                   -> pJAJ9 11200bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-SstI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   promoter        0..0
FT                   /note="PRO E. coli puf gene (rxcA)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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