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ID   PJAK14     preliminary; circular DNA; SYN; 11000 BP.
AC   ATCC77289;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli plasmid vector pJAK14 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJF118EH, pJF118HE from pMMB22 & pKK223-3
RC   pMMB66EH from RSF1010 & pJF118EH
RC   pMMB66HE from pMMB66EH & pJF118HE
RC   pJF119EH from pJF118EH & M13mp18
RC   pJF119HE from pJF118HE & M13mp18
RC   pMMB67EH from pMMB66EH & M13mp18
RC   pMMB67HE from pMMB66HE & M13mp18
RC   pMS324a from R300B & RP4
RC   pFG101 series from pJF118EH & pMS324a
RC   pFG102 series from pJF118HE & pMS324a
RC   pWP101 series from pMMB66EH & pMS324a
RC   pWP102 series from pMMB66HE & pMS324a
RA   Furste J.P., Pansegrau W., Frank R., Blocker H., Scholz P.,
RA   Bagdasarian M., Lanka E.;
RT   "Molecular cloning of the plasmid RP4 primase region in a
RT   multi-host-range tacP expression vector";
RL   Gene 48:119-131(1986).
RN   [2]
RC   pJAK14
RA   Kornacki J.A.;
RT   ;
RL   Submitted (00-JAN-1992) by:
RL   Kornacki J.A., .
CC   The order of the major features in this plasmid is: tac promoter -
CC   EcoRI/MCS/HindIII - rrnB terminator - kanR - oriV - oriT -
CC   RSF1010 ori - lacIq. (submitted, 1992)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--11.0; HindIII--11.0; PstI--8.2, 1.85, 1.0. (ATCC staff)
CC   A shuttle vector permitting autoregulated high-level expression in all
CC   gram-negative bacteria. (submitted, 1992)
CC   Differs from pJAK15 (ATCC 77290) only in the orientation of the
CC   polylinker. (submitted, 1992)
CC   Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013
CC   (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis,
CC   Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative
CC   bacteria. [1]
CC   Belongs to a group of vectors (ATCC 37622-37623, 77287-77292)
CC   differing only in antibiotic resistance and polylinker orientation.
CC   (submitted, 1992)
CC   Compatible with common E.coli cloning vectors, as well as vectors
CC   derived from IncP and IncW plasmids. (submitted, 1992)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pJAK14)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli SCS1)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. E. coli, tac promoter
FT                   2. E. coli, rrnB gene terminator
FT                   3. RSF1010 8684bp
FT                   4. E. coli, lambda lacIq gene
FT                   -> pJAK14 11000bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-Ecl136II-KpnI-XmaI-SmaI-BamHI-
FT                   XbaI-AccI-SalI-HincII-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SacI-Ecl136II-KpnI-BamHI-
FT                   XbaI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT   promoter        0..0
FT                   /note="PRO E. coli tac gene"
FT   CDS             0..0
FT                   /note="REP E. coli lacIq repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;