Return to this vector's summary.
ID PJB8 preliminary; circular DNA; SYN; 5464 BP.
XX
AC U00003; ATCC37074;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli cosmid vector pJB8 - incomplete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pJB8 from HomerI & linker
RA Ish-Horowicz D., Burke J.F.;
RT "Rapid and efficient cosmid cloning";
RL Nucleic Acids Res. 9:2989-2998(1981).
XX
RN [2]
RC pJB1, pJB2, pJB3 from pBR322 & S.typhimurium glnA gene
RC pJB1-B from pJB1
RC pJB5 from pJB1
RC pJB5, pJB6 from pJB1 & pBR322
RC pJB10 from pJB1-B
RC pJB8 from pJB10
RC pJB11, pJB12 from pJB8
RA Koduri R.K., Bedwell D.M., Brenchley J.E.;
RT "Characterization of a HindIII-generated DNA fragment carrying the
RT glutamine synthetase gene of Salmonella typhimurium";
RL Gene 11:227-237(1980).
XX
CC Restriction digests analyzed on agarose gels give the following
CC sizes (kb): HindIII--5.4; BamHI--5.4; PstI/HindIII--4.6, 0.8;
CC PstI/SalI--3.3, 2.3. (ATCC staff)
CC A cosmid vector containing a single cos site. [2]
CC Cloning into the BamHI or EcoRI site involves 2 preparations of
CC dephosphorylated vector, one linearized with HindIII, the other
CC linearized with SalI. [2]
CC An equimolar ratio of right-hand and left-hand cos containing vector
CC fragments, digested with BamHI or EcoRI can be ligated to
CC appropriately digested and dephosphorylated inserts. [2]
CC Only molecules with 2 cos sites separated by 37-50 kb can be
CC packaged. [2]
CC Medium is 1227 LB plus ampicillin.
CC NM (pJB8)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (ATCC)
CC HO (E.coli HB101)(E.coli)
CC CP ()
CC FN (cloning 30000 to 40000 bp)
CC SE ()
CC PA (Charon 4)(pAT153)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
XX
FT misc_feature 0..0
FT /note="1. pAT153 BamHI 3658bp 376..376
FT 2. Charon 4 BamHI/BstI-BamHI/BstI 1800bp, lambda cos
FT -> pJB8 5464bp
FT 1. HomerI EcoRI 5450bp 5449..5449
FT T4 DNA polymerase:T4 DNA polymerase
FT BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT remove BamHI-BamHI 6bp
FT -> pJB8 5464bp"
FT rep_origin 604..801
FT /note="ORI bacteriophage lambda"
FT misc_binding 604..801
FT /note="SIT bacteriophage lambda cos region"
FT rep_origin 3694..3694
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(4452..5312)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS complement(4452..5312)
FT /note="GEN Salmonella typhimurium glutamine
FT synthetase gene"
FT misc_binding 0..0
FT /note="SIT EcoRI, from pAT153 (5519..5519)"
FT misc_binding 0..0
FT /note="SIT BamHI, from BamHI linker"
FT misc_binding 0..0
FT /note="SIT EcoRI, from pAT153 (5519..5519)"
XX
SQ Sequence 5464 BP; 923 A; 1147 C; 1051 G; 986 T; 1357 other;
agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
ctgtggatcg atctgcctcg ctggcctgcc gcagttcttc aacctcccgg cgcagctttt
cgttctcaat ttcagcatcc ctttcggccg gcataccatt ttatgacggc ggcagagtca
taaagcacct cattaccctt gccaccgcct cgcagaacgg gcattccctg ttcctgccag
ttctgaatgg tacggatact cgcaccgaaa atgtcagcca gctgcttttt gttgacttcc
attgttcatt ccacggacaa aaacagagaa agcgacagag gccaaaaagc ctcgctttca
gcacctgtcg tttcctttct tttcagaggg taggtattta aataaaaaca ttaagttatg
acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga
ggtcgccgcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ctacgccgga
cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
catctgtatt aacgaagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttccggatcc
ggaa
//