ID   PJCF62     preliminary; DNA; SYN; 12100 BP.
XX
AC   L17027; ATCC86982;
XX
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Fremyella/E.coli plasmid vector pJCF62 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pFdA from Fremyella diplosiphon
RC   pJCF62 from pBR322 & pFdA
RA   Cobley J.G., Zerweck E., Reyes R., Mody A., Seludo-Unson J.R.,
RA   Jaeger H., Weerasuriya S., Navankasattusas S.;
RT   "Construction of shuttle plasmids which can be efficiently
RT   mobilized from Escherichia coli into the chromatically adapting
RT   cyanobacterium, Fremyella diplosiphon";
RL   Plasmid 30:90-105(1993).
XX
RN   [2]
RP   1-2938
RC   pFdA from Fremyella diplosiphon
RC   pJCF62 from pBR322 & pFdA
RA   Schaefer M.R., Chiang G.G., Cobley J.G., Grossman A.R.;
RT   "Plasmids from two morphologically distinct cyanobacterial strains
RT   share a novel replication origin";
RL   J. Bacteriol. 175:5701-5705(1993).
XX
CC   Shuttle vector. Constructed by inserting a 8.0 kb ClaI fragment,
CC   containing the F. diplosiphon pFDA origin of replication, into the
CC   AsuII site of pJCF22. Can be mobilized into F. diplosiphon using the
CC   conjugative plasmid RP4.
CC   The order of the major features of the plasmid is:  kanR - cmlR -
CC   F. diplosiphon pFDA ori - bom - oriV. [1]
CC   Growth media: LB + chloramphenicol (10 ug/ml) (ATCC number 1675) 37C
CC   Deposited by: J.G.Cobley
CC   NM (pJCF62)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(Fremyella diplosiphon)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. F. diplosiphon
FT                   -> pFDA
FT                   1. pBR322
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pJCF22 4100bp
FT                   1. pJCF22 AsuII 4100bp
FT                   2. pFdA ClaI-ClaI 8000bp, ori
FT                   -> pJCF62 12100bp"
FT   CDS             0..0
FT                   /note="ANT E. coli cat gene"
FT   CDS             0..0
FT                   /note="ANT Fremyella diplosiphon kanR gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Fremyella diplosiphon pFDA"
XX
SQ   Sequence 2938 BP; 888 A; 618 C; 655 G; 777 T; 0 other;
     ggatcctcta gagtcaattc acttattaat actttgttat gcctaaagag gggcgaattc
     tggcagtcct agaaactgaa gaaaaggaga aattaaaaaa aatagctgcg gctcaaagtc
     gctcactgtc tagcttagta gcgtttatat tacgcgagtg gctagctaaa caagaaaatg
     actagccacg tatatacatg gttatttaat tttttttctg atgcgcttgt atgctggcgg
     ggcgattgct gggttgccaa catgatctgg caagtcctgc caggataacc ctaattcttt
     taaatagata tctaatcgct tgatttgggc gatagttagc gctacgttaa cttttgaatt
     ctcccatttc cctattgtat cggcatcaat acccagttcc agcccaaact ggctacgtga
     cagtccaagg gctttccgaa tttgtaaaaa agccgacacg gtactatcaa gcgaaaaccc
     ttcattttcg cttatcataa atttgaagtg cgctagagag cggtatttcc cagtagccgc
     aaactactgg aatggtagac acagggcgat tttaaatcgc cccattaatt ccttaattca
     attaggagga aattgcaagc aataaatgca ataaacgcta caacaccatg acaacctgac
     atgaagcgac acataaaaaa atgacttgca gacacatcaa aaaacccaga cgcgccaaca
     agccaacaaa aataaatctc taactaaaaa ttaacaaata ttgagcgtga aaatataaag
     ctgttggaaa gcttggggtg atgtgtcagc aagtcgctat aaaaggggcg atttgtgaaa
     catactacat ttccacattc caatattata gcacaatcca ccgctacaga gggggagaat
     tttgtactac aaacaagatt acacgcttca attctcctgc tcactccggc ttctaaattg
     tggtatttgt gtcgagcgtt ggatctgagt ggtcgtggtt atgtagtttt gtctcctcaa
     aatctggaac tcttgcagga aaaaaaatca acaatttacc gttggctcaa ggatggtaag
     gatttggggc tatttcgttg ttactcctgg gcggggaaca ctttaaaaat tagcctgggt
     agcctgtggc gcgcttgcaa aaaagcaaga ataaaaaatt ggggcacagt ggccaacaac
     atcccactag aagaaatttt aaaaaataat ggtcgtcgca ctgtcgctac agcgatgaca
     attcaagatt ggcaagagcg ctcacgatac gcggcaaaaa atcaacttaa cccgcttgag
     cgtaaatgtt ttgagattcc ggctactgcc gacgtgctaa caccccaaac ctcaccgaag
     ctgactagtg ggggaacaac tggagtgtta cacgttggcg agaagcggat ttttgttggc
     cgctcgttca tcccctttgg ggtttctcag aagcggatca gcaatgagct aaactcccag
     ccggccagct gtggtgtttc agttcgcaca gtccaaagac acgttgagcg tttggaggta
     aaacacaggc aattgatgca agcccgccgg gaataccggg aaatcgctca cagaatcaag
     cagggggcta ctagctggca gtgcaagagt gatgctgaca tcagcttcac ctggggcaat
     cagcccgatg aaattgtact ccatgagaga aacgggaaat catcagctag gcgtgagggg
     gggcacacaa tcaaactgga tcagctgtgc aactacagca atacacattg gcgctatcgc
     tgtaatttgt accttctcag ctacgagttg acctcgatga ggacaactcg ctatcagtgg
     aagaaacgcg ggttaaacgt caaaattgca cctgtggaaa actgcccaag tgagatttcc
     ccccaaaccc ccgaaatgct tgaatccccc aaacttcacg ccgaagggcg tggtttgggg
     ggtcagaata agagtgtcaa aaatgagaat cccaggaagt agacacagta agcaatgcgt
     tcgctccagg ggttctgagt gggcaaaact aaggctatgc tactggccaa gcaagccgag
     agaaggcaaa aacgatggat agtctgaact ctatatgatg taagcatttc agctatttat
     tcacaggcga tcgctttttt gaagcgatga gattatgctg ggcgaagtga ttttttactt
     aaaacaaagg tatttttatg cccttattta atcctgtgag cgccactagc gccactagta
     gcagtggaaa caagctaact aactcattaa gtacaacggc ttctaaaatc gctaatagca
     acacaaaccg caagggctta acaattcaca atcctttatc agtaaccgta tatattgata
     ctgctgacac cgtaactagt agtgcttgtt gggtaccaat cactgcgggg gcttattttg
     aattacctac caatgggatt tacacgggcg aaatttgggc gattactgct agtggtacgg
     ggtcagttaa tgctagggaa tttagttaat tatgccttta tatcctgctg caactcctgc
     taataataat gtcagtcgct caagcgaaag tattactgtg tctgctccct tggctaataa
     ttcctatcaa ttaattgatg tggtattagc taaaagtttt ttgctattag cgattaattg
     ccctatagcc gcaagaatca ggctttactt tgataatgtt tctagagcca gtgatttatc
     aagggtaata ggaagtgatg ctgatagctc aattgcaatc attgcagaag taattactag
     cgctagcttg caaactatta atttggctcc ttgtgctagt ggatttaatg ctaatactac
     taatccctat tatttaagct ttactaattt aagcggtagc gctattaata gcgatatc
//