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ID   PJD220SVHY preliminary; circular DNA; SYN; 5100 BP.
AC   ATCC67393;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pJD220SVHy - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJD220SVHy from pJD217SVHy & SV40 polyA
RA   ;
RT   ;
RL   Unpublished (1990).
RL   U.S. Patent No. 4,980,289 dated Dec. 25, 1990.
RN   [2]
RC   pJD214 from pUC12 & spleen necrosis virus
RC   pJD214Hy from pJD214 & hygro gene
RC   pJD214Neo from pJD214 & neo gene
RC   pJD216NeoHy from pJD214Hy & neo gene & Rev-A splice acceptor
RC   pJD216HyNeo from pJD214Neo & Rev-A splice acceptor & hygro gene
RC   pJD216NeoHypr from pUC12 & pJD216NeoHy
RC   pJD216HyNeopr from pUC12 & pJD216HyNeo
RA   Dougherty J.P., Temin H.M.;
RT   "High mutation rate of a spleen necrosis virus-based retrovirus
RT   vector";
RL   Mol. Cell. Biol. 6:4387-4395(1986).
RN   [3]
RC   pJD217Hy from pJD214Hy & linker
RC   pJD217SVHy from pJD217Hy & pSV2-neo
RC   pJD220Hy from pJD217Hy & SV40 polyA
RC   pJD220SVHy from pJD217SVHy & SV40 polyA
RA   Dougherty J.P., Temin H.M.;
RT   "A promoterless retroviral vector indicates that there are
RT   sequences in U3 required for 3' RNA processing";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:1197-1201(1987).
RN   [4]
RC   pJD214HY from pJD214 & hygro gene
RC   pJD220SVHY from pJD220Hy & SV40 polyA
RC   pRD17 from pRD8 & pJE189
RC   pRD18 from pRD8 & pTK1 & pJE189
RC   pRD19 from pRD8 & pJE189
RA   Dornburg R., Temin H.M.;
RT   "Retroviral vector system for the study of cDNA gene formation";
RL   Mol. Cell. Biol. 8:2328-2334(1988).
RN   [5]
RC   [pME111 from pME series]
RC   pME111-2 from pME111 & turkey c-rel gene
RC   pME111-1 from pME111-2
RC   [pME123 from pME series]
RC   pME149 from pME123 & MLV U3 region
RC   pME139 from pME111 & AFVXM virus
RC   pME140 from pME123 & AFVXM virus
RC   pME151 from pME140 & AFVXM virus
RA   Emerman M., Temin H.M.;
RT   "Comparison of promoter suppression in avian and murine retrovirus
RT   vectors";
RL   Nucleic Acids Res. 14:9381-9396(1986).
RN   [6]
RC   lambda MLV-1 from Charon 21A & MoMuLV
RC   pMLV-1 from pBR322 & lambda MLV-1
RC   pME142 from pME123 & AFVXM virus
RA   Shinnick T.M., Lerner R.A., Sutcliffe J.G.;
RT   "Nucleotide sequence of Moloney murine leukemia virus";
RL   Nature 293:543-548(1981).
RN   [7]
RC   pJE189 from MoMuLV LTR
RA   Embretson J.E., Temin H.M.;
RT   "Pseudotyped retroviral vectors reveal restrictions to
RT   reticuloendotheliosis virus replication in rat cells";
RL   J. Virol. 60:662-668(1986).
CC   pJD214Hy was constructed from 1.45 kb of SNV DNA ligated into the
CC   BamHI/EcoRI (nt 2066 to nt 4361) fragment of pBR322, a pUC12
CC   polylinker, and the hygromycin phosphotransferase B gene (BamHI
CC   fragment blunt end-ligated into the HindIII site of the MCS). [2]
CC   An intermediate, pJD217SVHy, was constructed from pJD214Hy by deleting
CC   a SacI/AvaI fragment (SNV map units 7.747-8.127) and replacing it with
CC   an XhoI linker, and inserting a 565 bp NdeI/HindIII fragment from
CC   pSV2-neo (SV40 promoter) at the XbaI site. [3]
CC   Constructed from pJD217SVHy by inserting a 220 bp BamHI/HindIII
CC   fragment (SV40 poly(A) site) into the BamHI site at the 3' end of U5.
CC   [3]
CC   A replication-defective retroviral vector which is promoter-deficient
CC   in the right LTR and cannot express retroviral RNA.  Foreign gene
CC   expression is regulated by the SV40 early promoter. [3]
CC   Removal of SNV map units 7.747 to 8.127 results in deletion of the
CC   entire U3 sequence, except for 10 bp at the 5' end containing the attL
CC   site. [1]
CC   The 5' 10 bp of U3 in the left LTR were deleted, so there is no
CC   homology between the two U3 sequences and the risk of recombination is
CC   reduced. [1]
CC   pJD220SvHy (ATCC 67393) and pRD8 (ATCC 67394) differ in the
CC   orientation of the SV40 promoter/hygromycin phosphotransferase B
CC   sequences.  pRD8 has an additional 3' RNA processing sequence between
CC   the left U5 and the hygromycin sequences. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--5.0, 0.4; PstI--2.7, 2.3, 0.4; XbaI--5.2. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pJD220SVHy)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(vertebrate cells)(E.coli)
CC   HO (D17-C3 helper cell line for transfection)
CC   HO (D17 cell line for infection)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC12 2680bp, MCS
FT                   2. spleen necrosis virus 1450bp
FT                   -> pJD214 4130bp
FT                   1. pJD214 HindIII 4130bp, pUC12 276..276
FT                   blunt end:blunt end
FT                   2. E. coli BamHI-BamHI 1150bp, hyg gene
FT                   blunt end:blunt end
FT                   -> pJD214Hy 5300bp
FT                   1. pJD214Hy remove SacI-AvaI 7.747 to 8.127, SNV 3'LTR
FT                   \ 4300bp
FT                   XhoI linker 6bp ctcgag
FT                   -> pJD217Hy 4300bp [no U3]
FT                   1. pJD217Hy XbaI 4300bp
FT                   2. pSV2-neo HindIII-NdeI 574bp 3093..3667,
FT                   \ SV40 early promoter [565bp]
FT                   -> pJD217SVHy 4900bp
FT                   1. pJD217SVHy BamHI 4900bp, 3' U5
FT                   2. SV40 BamHI-HindIII 237bp 2534..2771, polyA
FT                   -> pJD220SVHy 5100bp"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SmaI-XmaI-BamHI-XbaI-SalI-PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI-SalI-ClaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;