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ID   PJEL170    preliminary; circular DNA; SYN; 12500 BP.
AC   X05629; ATCC37529;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pJEL170 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pVH7 from pBR322 & deoCABD genes
RC   pJEL153 from pJEL75 & pPR633
RC   pJEL154 from pJEL153 & pBR322
RC   pJEL170 from pJEL126 & pJEL154
RC   pJEL144 from pJEL134 & pJEL170
RC   pJEL147 from pVH7 & pJEL170
RC   pJEL141, pJEL143, pJEL145, pJEL146 from pVH7 & pJEL144
RA   Larsen J.E., Albrechtsen B., Valentin-Hansen P.;
RT   "Analysis of the terminator region after the deoCABD operon of
RT   Escherichia coli K-12 using a new class of single copy number
RT   operon-fusion vectors";
RL   Nucleic Acids Res. 15:5125-5140(1987).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--13.0; EcoRI--13.0. (ATCC staff)
CC   Below 37C, there is one copy per genome equivalent stabilized by the
CC   parB locus.  At 42C replication is uncontrolled because induction of
CC   the cI857-regulated PR promoter permits expression of the copB, copA,
CC   and repA genes.
CC   A promoter-cloning vector using increased expression of
CC   beta-galactosidase as the reporter.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pJEL170)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli CSH50)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   4360..4361..376, 3984bp
FT                   2. R1 EcoRI-BamHI 580bp, 0 to +580 parB gene
FT                   -> pPR633 4564bp
FT                   1. R1
FT                   2. lambda, pR/cI857 gene
FT                   -> pOU71
FT                   1. pOU71 remove HindIII-HindIII
FT                   -> pJEL75
FT                   1. pJEL75 NdeI-BamHI
FT                   Klenow:
FT                   2. pPR633 EcoRI-BamHI 580bp, 0 to +580 parB gene
FT                   Klenow:
FT                   -> pJEL153
FT                   1. pJEL153 remove small BamHI-ClaI
FT                   2. pBR322 XhoII-ClaI 1269bp 3117..4361..25, amp gene
FT                   -> pJEL154
FT                   1. lambda, cI857 gene
FT                   2. pMC903, lac gene
FT                   3. R1, parA gene
FT                   -> pJEL126
FT                   1. pJEL126 EcoRI-HindIII, lac gene/cI857
FT                   2. pJEL154 large EcoRI-HindIII
FT                   -> pJEL170 12500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli R1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;