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ID   PJG200     preliminary; circular DNA; SYN; 1 BP.
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AC   IG7019;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector pJG200 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pMLB1034 & lambda, pR/cI857/RBS/cro gene ATG
RC   pJG200 from plasmid & chicken proalpha-2-collagen gene
RC   pJG201 from pJG200 & R6K & linker
RA   Germino J., Bastia D.;
RT   "Rapid purification of a cloned gene product by genetic fusion and
RT   site-specific proteolysis";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:4692-4696(1984).
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RN   [2]
RC   pJG10 from pJG100 & pMLB1031
RC   pJG11 from pJG100 & pMLB1031
RC   pJG14 from pUC9 & R6K, ori gamma
RC   pJG160 from pJG10 & R6K, ori gamma
RA   Germino J., Gray J., Charbonneau H., Vanaman T., Bastia D.;
RT   "Use of gene fusions and protein-protein interaction in the
RT   isolation of a biologically active regulatory protein: the
RT   replication initiator protein of plasmid R6K";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:6848-6852(1983).
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RN   [3]
RC   pJG4 from R6K & pBR322
RC   M13mp7-I, M13mp7-I* from pJG4 & M13mp7
RC   pJG50 from M13mp7-I & pUC9
RC   pJG100 from M13mp7-I & pUC9
RC   pJG150 from pJG50 & pJG100
RC   pJG5 from pJG4
RC   pJG6, pJG7 from pJG4 & pUC9
RA   Germino J., Bastia D.;
RT   "The replication initiator protein of plasmid R6K tagged with
RT   beta-galactosidase shows sequence-specific DNA-binding";
RL   Cell 32:131-140(1983).
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CC   NM (pJG200)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMLB1034 large EcoRI-BamHI
FT                   2. lambda EcoRI-BamHI, pR/cI857/RBS/cro gene ATG
FT                   -> plasmid
FT                   1. plasmid BamHI
FT                   2. chicken Sau3A-Sau3A, proalpha-2-collagen gene
FT                   -> pJG200"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO phage lambda PR"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_feature    0..0
FT                   /note="SIT collagen linker"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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