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ID   PJII102    preliminary; RNA; SYN; 117 BP.
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AC   M19103; A12498; A12499; A06006; A06007;
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DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Tobacco plasmid vector pJII102 - incomplete, TMV/pJII2 cat 5'.
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KW   cloning vector; tobacco mosaic virus; chloramphenicol transferase.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-117
RC   pJII102 from TMV
RC   pJII103 from TMV
RC   pJII120 from TMV
RC   pJII140 from TMV
RA   Sleat D.E., Gallie D.R., Jefferson R.A., Bevan M.W., Turner P.C.,
RA   Michael T., Wilson A.;
RT   "Characterisation of the 5'-leader sequence of tobacco mosaic virus
RT   RNA as a general enhancer of translation in vitro";
RL   Gene 60:217-225(1987).
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CC   TMV = tobacco mosaic virus
CC   NCBI gi: 209265
CC   NM (pJII102)
CC   CM (no)
CC   NA (RNA)
CC   TP (linear)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJII2)(tobacco mosaic virus)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. TMV
FT                   -> pJII2
FT                   1. pJII2, TMV
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pJII102"
FT   misc_recomb     12..13
FT                   /note="plasmid RNA end/viral RNA start"
FT   misc_recomb     79..80
FT                   /note="viral RNA end/plasmid RNA start"
FT   CDS             114..>117
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   NCBI gi: 209266"
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SQ   Sequence 117 BP; 52 A; 22 C; 14 G; 29 T; 0 other;
     gaatacaagc tttattttta caacaattac caacaacaac aaacaacaaa caacattaca
     attactattt acaattacag tcgacgagat tttcaggagc taaggaagct aaaatgg
//