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ID   PJII103    preliminary; RNA; SYN; 121 BP.
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AC   M19104; A12500; A06008;
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DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Tobacco plasmid vector pJII103 - incomplete, TMV/pJII3 neo 5'.
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KW   cloning vector; tobacco mosaic virus; neomycin phosphotransferase II.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-121
RC   pJII102 from TMV
RC   pJII103 from TMV
RC   pJII120 from TMV
RC   pJII140 from TMV
RA   Sleat D.E., Gallie D.R., Jefferson R.A., Bevan M.W., Turner P.C.,
RA   Michael T., Wilson A.;
RT   "Characterisation of the 5'-leader sequence of tobacco mosaic virus
RT   RNA as a general enhancer of translation in vitro";
RL   Gene 60:217-225(1987).
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CC   TMV = tobacco mosaic virus
CC   NCBI gi: 209271
CC   NM (pJII103)
CC   CM (no)
CC   NA (RNA)
CC   TP (linear)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJII3)(tobacco mosaic virus)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. TMV
FT                   -> pJII3
FT                   1. pJII3, TMV
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pJII103"
FT   misc_recomb     12..13
FT                   /note="plasmid RNA end/viral RNA start"
FT   misc_recomb     79..80
FT                   /note="virus RNA end/plasmid RNA start"
FT   CDS             118..>121
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII); neomycin resistance gene (neo);
FT                   NCBI gi: 209272"
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SQ   Sequence 121 BP; 51 A; 24 C; 15 G; 31 T; 0 other;
     gaatacaagc tttattttta caacaattac caacaacaac aaacaacaaa caacattaca
     attactattt acaattacag tcgatctgat caagagacag gatgaggatc gtttcgcatg
     a
//