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ID   PJII140    preliminary; RNA; SYN; 90 BP.
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AC   M19106; A12510; A12511;
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DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Higher plants virus vector pJII140 - incomplete, TMV/pJII139 5' GUS.
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KW   cloning vector; beta-glucuronidase; GUS.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-90
RC   pJII102 from TMV
RC   pJII103 from TMV
RC   pJII120 from TMV
RC   pJII140 from TMV
RA   Sleat D.E., Gallie D.R., Jefferson R.A., Bevan M.W., Turner P.C.,
RA   Michael T., Wilson A.;
RT   "Characterisation of the 5'-leader sequence of tobacco mosaic virus
RT   RNA as a general enhancer of translation in vitro";
RL   Gene 60:217-225(1987).
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CC   TMV = tobacco mosaic virus
CC   NCBI gi: 209553
CC   NM (pJII140)
CC   CM (no)
CC   NA (RNA)
CC   TP (linear)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (tobacco mosaic virus)(pJII139)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. TMV
FT                   -> pJII139
FT                   1. pJII139, TMV
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pJII140"
FT   misc_recomb     12..13
FT                   /note="plasmid RNA end/viral RNA start"
FT   misc_recomb     79..80
FT                   /note="viral RNA end/plasmid RNA start"
FT   CDS             87..>90
FT                   /note="GEN E. coli beta-glucuronidase gene (GUS);
FT                   NCBI gi: 209554"
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SQ   Sequence 90 BP; 41 A; 20 C; 6 G; 23 T; 0 other;
     gaatacaagc tttattttta caacaattac caacaacaac aaacaacaaa caacattaca
     attactattt acaattacag tcgaccatgg
//