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ID   PJIR750    preliminary; circular DNA; SYN; 6570 BP.
AC   ATCC87015;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Clostridium perfringens/E.coli plasmid pJIR750 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJIR145 from pJIR71 & Tn1725, tet gene
RC   pJIR274 from pUC18 & pJIR62, C.perfringens cat gene
RC   pJIR275 from pJIR274 & pJIR71, C.perfringens tet gene
RC   pJIR282 from pJIR145 & pJIR275
RC   pJIR292 from pJIR229 & pJIR275
RC   pJIR297 from pJIR292 & pIP404
RC   pJIR335 from pJIR297
RC   pJIR403 from pJIR335
RC   pJIR408 from pJIR403
RC   pJIR415 from pJIR408
RC   pJIR418 from pJIR415 & pUC18
RC   [pJIR750 from pJIR418 & C. perfringens, cat gene]
RA   Sloan J., Warner T.A., Scott P.T., Bannam T.L., Berryman D.I.,
RA   Rood J.I.;
RT   "Construction of a sequenced Clostridium perfringens - Escherichia
RT   coli shuttle plasmid";
RL   Plasmid 27:207-219(1992).
RN   [2]
RC   pJIR418 from pJIR415 & pUC18
RC   pJIR750 from pJIR418 & C. perfringens, cat gene
RA   Bannam T.L., Rood J.I.;
RT   "Clostridium perfringens-Escherichia coli shuttle vectors that
RT   carry single antibiotic resistance determinants";
RL   Plasmid 29:233-235(1993).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BglI--4.4, 2.2; ClaI--6.6; EcoRV--6.6. (ATCC staff)
CC   The standard reverse sequencing primer is not useful in this
CC   vector due to the duplication of the 181 bp region between pUC18 sites
CC   PvuII and EcoRI. [1]
CC   Shuttle expression vector derived from pJIR418 (ATCC 77387) permitting
CC   expression of antibiotic resistance in both hosts. The chloramphenicol
CC   resistance gene (catP), expressed in both C. perfringens and E. coli,
CC   was derived from C. perfringens and is inactivated by insertions at
CC   the EcoRV site. The vector contains the following restriction sites
CC   (approximate kb from the EcoRI site):  BglI--0.20, 2.50; ClaI--4.99.
CC   5.03; EcoRV--4.72; NdeI--0.27, 2.09; PvuI--0.17; PvuII--0.14, 6.39;
CC   SmaI--4.98. The order and orientations of the major features of the
CC   plasmid are:  lacZ' - EcoRI/MCS/HindIII lacZ' -> - pIP404 ori -
CC   <- rep - <- catP - pMB1 ori. [2]
CC   Growth: LB plus chloramphenicol (10 ug/ml) (ATCC med num 1675) 37C
CC   Deposited by: Rood J.I.
CC   NM (pJIR750)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(Clostridium perfringens)
CC   CP ()
CC   FN (cloning)(expression)(fusion)(shuttle)
CC   SE (color)
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. C.perfringens, cat gene
FT                   -> pIP4044 10200bp
FT                   1. pJIR418 remove, 7358bp-
FT                   2. pIP404, C. perfringens cat gene
FT                   -> pJIR750 6570bp"
FT   rep_origin      0..0
FT                   /note="ORI Clostridium perfringens pIP404"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT C. perfringens cat gene"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SacI-KpnI-BamHI-XbaI-SalI-PstI-
FT                   SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRV"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;