ID   PJK142     preliminary; DNA; SYN; 3270 BP.
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AC   ATCC86955;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pJK142 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJK104, pJK105, pJK107, pJK125, pJK142 from pBR322 & S.pombe DNA
RC   pJK148, pJK190, pJK195, pJK210, pJK220 from pBR322 & S.pombe DNA
RA   Keeney J.B., Boeke J.D.;
RT   "Efficient targeted integration at leu1-32 and ura4-294 in
RT   Schizosaccharomyces pombe";
RL   Genetics 136:849-856(1994).
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CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: J.B.Keeney
CC   NM (pJK142)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. E. coli, lac promoter/lac gene
FT                   2. S.pombe DNA
FT                   -> pJK142 3270bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="MCS unique SacI-SacII-SpeI-XbaI-NotI-BamHI-
FT                   SmaI-PstI-EcoRI-EcoRV-HindIII-ClaI-SalI-HincII-
FT                   XhoI-ApaI-DraII-KpnI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli lac gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//