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ID PJK148 preliminary; DNA; PLN; 5343 BP.
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AC L25927; ATCC86956;
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DT 01-DEC-1994 (Rel. 10, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Schizosaccharomyces/E.coli plasmid vector pJK148 - incomplete.
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KW cloning vector.
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OS Schizosaccharomyces pombe
OC Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Endomycetales;
OC Schizosaccharomycetoideae.
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RN [1]
RP 1-5343
RC pJK104, pJK105, pJK107, pJK125, pJK142 from pBR322 & S.pombe DNA
RC pJK148, pJK190, pJK195, pJK210, pJK220 from pBR322 & S.pombe DNA
RA Keeney J.B., Boeke J.D.;
RT "Efficient targeted integration at leu1-32 and ura4-294 in
RT Schizosaccharomyces pombe";
RL Genetics 136:849-856(1994).
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CC Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC Deposited by: J.B.Keeney
CC NM (pJK148)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli)(Schizosaccharomyces pombe)
CC CP ()
CC FN (cloning)(shuttle)(integrating)(leu1)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 4361bp, ori/amp gene
FT 2. E. coli, lac promoter/lac gene
FT 2. S.pombe, LEU1 gene
FT -> pJK148 5343bp"
FT CDS 0..0
FT /note="ANT E. coli amp gene"
FT CDS 0..0
FT /note="ANT yeast leu1 gene"
FT misc_binding 0..0
FT /note="MCS SacI-SacII-NotI-XbaI-SpeI-BamHI-SmaI-
FT PstI-EcoRI-EcoRV-HindIII-ClaI-SalI-HincII-XhoI-
FT ApaI-DraII-KpnI"
FT misc_binding 0..0
FT /note="MCS unique SacI-SacII-NotI-XbaI-SpeI-BamHI-
FT SmaI-PstI-EcoRI-EcoRV-SalI-HincII-ApaI-DraII-KpnI"
FT promoter 0..0
FT /note="PRO E. coli lac"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="GEN E. coli lac gene"
XX
SQ Sequence 5343 BP; 1390 A; 1208 C; 1290 G; 1455 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatacgat atcccaatct gtagtataaa aagtgcaaca gtgttgagtt tcccgaaacc
aggcattttt gcggtagggt ctgaaacttt cacgaaacca tttcacaaca aaacttcttc
aacaacatgt gtgcaaagaa aatcgtcgtc ttaccaggag accatattgg ccctgaaatt
gttgcttctg ccttggaggt tttgaaagtc gttgagaaga agcgacctga gttaaaactc
gagtttgaag aacacaagat tggaggtgcc tctattgatg cctatggaac ccctttgact
gacgagactg tgaaggcttg tttggaagct gacggtgttc ttttgggtgc cgttggtggt
cctgaatgga ccaaccccaa ttgtcgtcct gagcaaggtt tattgaagct tcgtaagagt
atgggtgttt gggccaacct tcgaccttgc aactttgcca gcaagtcttt agtcaagtac
agccctttga agcctgaaat cgttgaaggt gtcgattttt gtgttgtacg agaacttact
ggaggttgtt actttggtga gcgcactgag gacaacggat cgggttatgc tatggacact
tggccttaca gtttggaaga agtttctcgt attgctcgtt tggctgcttg gttagctgaa
acttccaacc ctcctgctcc cgtcacatta ctcgacaaag ctaatgtttt ggcaacttct
cgtctttggc gcaagactgt agctaagatt tttaaggagg aatatcctca ccttacctta
aaaaaccagc tcattgactc cgcagccatg cttttggtca agagccctcg tacacttaac
ggtgttgttt tgactgacaa cttgtttggt gacattattt cagatgaggc ttctgtcatt
cctggtagct tgggcctttt gccttctgcc tccctttccg gtgtggtagg aaaatcagaa
gaaaaggttc attgtttggt tgagcccatt cacggtagcg ctcccgatat cgctggtaag
ggcattgtta atcctgttgg tacaatttta tctgcttccc ttctccttcg ttatggtttg
aatgctccta aggaggctga agctatcgaa gccgccgtac gcaaggtctt ggatgatact
tcaattggtg gacgtggtct ttatactcgc gatttgggag gtgaggcttc taccgctgat
attactaagg ctgttgttga agaacttgaa aaaattttgt aatagggttt aatgtagaat
aaattcatat gatttgattt gacctaattt ttattgatta attgtgtcgc atatttatta
tcgttaaaaa tgcaattgac aaaagcgtaa attttaaggc tctaatgttt tttcttttgg
tatgttatag tcaagcaaag atgtattaag ttgcaaaagc atggaattga tttacgacat
ccgttcaact ttatgaccga gttgtcgctg tgtacttact ctttttttac tatgaaaagt
ttcaaacata atatcattaa ttatttcttt ttaaacgaag taacctgtaa cataaataat
aggggaaaag aagaaaaaaa ccataaaaat tcgagaatcg taacaattat tattgtaacg
atgacaaccc gattctttga gagagccaga gacatgactt ttgcattacc gtaattggtt
tctacttatg aattgtaaac ttgtgtaggg aatttcatcc accagctatc gcgggctgct
ctctgcatca gtgttagcac attttatatt aatgaagatt ttgctatgca aaatacgtaa
atttctggcg cgatatattt ttatcaaacg aacatgacta tcctttccac tttgatggtt
cgcaagtatt ttgatttgta ttaaatgcac atctaacaag ccatttcgcc caaaatcacc
aactggcaca tgtacccaac cagttaggtt cctcgacttg aactgttgta acaatttcca
aatcttgaaa ccctgtccca gctgatattc gcaaagtact gggagtataa gattcatcaa
gggtgtattg aaggtacata ctcacatact taatcgatgc ggtgtgaaat accgcacaga
tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg
cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
cgtcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg cggtgcgggc
ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg
cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctcggcc
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc
tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata actacgatac
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
gtc
//