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ID   PJK148     preliminary; DNA; PLN; 5343 BP.
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AC   L25927; ATCC86956;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Schizosaccharomyces/E.coli plasmid vector pJK148 - incomplete.
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KW   cloning vector.
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OS   Schizosaccharomyces pombe
OC   Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Endomycetales;
OC   Schizosaccharomycetoideae.
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RN   [1]
RP   1-5343
RC   pJK104, pJK105, pJK107, pJK125, pJK142 from pBR322 & S.pombe DNA
RC   pJK148, pJK190, pJK195, pJK210, pJK220 from pBR322 & S.pombe DNA
RA   Keeney J.B., Boeke J.D.;
RT   "Efficient targeted integration at leu1-32 and ura4-294 in
RT   Schizosaccharomyces pombe";
RL   Genetics 136:849-856(1994).
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CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: J.B.Keeney
CC   NM (pJK148)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(Schizosaccharomyces pombe)
CC   CP ()
CC   FN (cloning)(shuttle)(integrating)(leu1)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. E. coli, lac promoter/lac gene
FT                   2. S.pombe, LEU1 gene
FT                   -> pJK148 5343bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast leu1 gene"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SacII-NotI-XbaI-SpeI-BamHI-SmaI-
FT                   PstI-EcoRI-EcoRV-HindIII-ClaI-SalI-HincII-XhoI-
FT                   ApaI-DraII-KpnI"
FT   misc_binding    0..0
FT                   /note="MCS unique SacI-SacII-NotI-XbaI-SpeI-BamHI-
FT                   SmaI-PstI-EcoRI-EcoRV-SalI-HincII-ApaI-DraII-KpnI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli lac gene"
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SQ   Sequence 5343 BP; 1390 A; 1208 C; 1290 G; 1455 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatacgat atcccaatct gtagtataaa aagtgcaaca gtgttgagtt tcccgaaacc
     aggcattttt gcggtagggt ctgaaacttt cacgaaacca tttcacaaca aaacttcttc
     aacaacatgt gtgcaaagaa aatcgtcgtc ttaccaggag accatattgg ccctgaaatt
     gttgcttctg ccttggaggt tttgaaagtc gttgagaaga agcgacctga gttaaaactc
     gagtttgaag aacacaagat tggaggtgcc tctattgatg cctatggaac ccctttgact
     gacgagactg tgaaggcttg tttggaagct gacggtgttc ttttgggtgc cgttggtggt
     cctgaatgga ccaaccccaa ttgtcgtcct gagcaaggtt tattgaagct tcgtaagagt
     atgggtgttt gggccaacct tcgaccttgc aactttgcca gcaagtcttt agtcaagtac
     agccctttga agcctgaaat cgttgaaggt gtcgattttt gtgttgtacg agaacttact
     ggaggttgtt actttggtga gcgcactgag gacaacggat cgggttatgc tatggacact
     tggccttaca gtttggaaga agtttctcgt attgctcgtt tggctgcttg gttagctgaa
     acttccaacc ctcctgctcc cgtcacatta ctcgacaaag ctaatgtttt ggcaacttct
     cgtctttggc gcaagactgt agctaagatt tttaaggagg aatatcctca ccttacctta
     aaaaaccagc tcattgactc cgcagccatg cttttggtca agagccctcg tacacttaac
     ggtgttgttt tgactgacaa cttgtttggt gacattattt cagatgaggc ttctgtcatt
     cctggtagct tgggcctttt gccttctgcc tccctttccg gtgtggtagg aaaatcagaa
     gaaaaggttc attgtttggt tgagcccatt cacggtagcg ctcccgatat cgctggtaag
     ggcattgtta atcctgttgg tacaatttta tctgcttccc ttctccttcg ttatggtttg
     aatgctccta aggaggctga agctatcgaa gccgccgtac gcaaggtctt ggatgatact
     tcaattggtg gacgtggtct ttatactcgc gatttgggag gtgaggcttc taccgctgat
     attactaagg ctgttgttga agaacttgaa aaaattttgt aatagggttt aatgtagaat
     aaattcatat gatttgattt gacctaattt ttattgatta attgtgtcgc atatttatta
     tcgttaaaaa tgcaattgac aaaagcgtaa attttaaggc tctaatgttt tttcttttgg
     tatgttatag tcaagcaaag atgtattaag ttgcaaaagc atggaattga tttacgacat
     ccgttcaact ttatgaccga gttgtcgctg tgtacttact ctttttttac tatgaaaagt
     ttcaaacata atatcattaa ttatttcttt ttaaacgaag taacctgtaa cataaataat
     aggggaaaag aagaaaaaaa ccataaaaat tcgagaatcg taacaattat tattgtaacg
     atgacaaccc gattctttga gagagccaga gacatgactt ttgcattacc gtaattggtt
     tctacttatg aattgtaaac ttgtgtaggg aatttcatcc accagctatc gcgggctgct
     ctctgcatca gtgttagcac attttatatt aatgaagatt ttgctatgca aaatacgtaa
     atttctggcg cgatatattt ttatcaaacg aacatgacta tcctttccac tttgatggtt
     cgcaagtatt ttgatttgta ttaaatgcac atctaacaag ccatttcgcc caaaatcacc
     aactggcaca tgtacccaac cagttaggtt cctcgacttg aactgttgta acaatttcca
     aatcttgaaa ccctgtccca gctgatattc gcaaagtact gggagtataa gattcatcaa
     gggtgtattg aaggtacata ctcacatact taatcgatgc ggtgtgaaat accgcacaga
     tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg
     cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     cgtcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg cggtgcgggc
     ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
     cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
     gcttgatatc gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
     ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag
     ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg
     cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctcggcc
     cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata actacgatac
     gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     gtc
//