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ID   PJK210     preliminary; DNA; PLN; 5032 BP.
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AC   L25928; ATCC86957;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   SchizosaccharomycesE.coli plasmid vector pJK210 - incomplete.
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KW   cloning vector.
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OS   Schizosaccharomyces pombe
OC   Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Endomycetales;
OC   Schizosaccharomycetoideae.
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RN   [1]
RP   1-5032
RC   pJK104, pJK105, pJK107, pJK125, pJK142 from pBR322 & S.pombe DNA
RC   pJK148, pJK190, pJK195, pJK210, pJK220 from pBR322 & S.pombe DNA
RA   Keeney J.B., Boeke J.D.;
RT   "Efficient targeted integration at leu1-32 and ura4-294 in
RT   Schizosaccharomyces pombe";
RL   Genetics 136:849-856(1994).
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CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: J.B.Keeney
CC   NM (pJK210)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(Schizosaccharomyces pombe)
CC   CP ()
CC   FN (cloning)(shuttle)(integrating)(ura4 dihydroorotase)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. E. coli, lac promoter/lac gene
FT                   2. S.pombe, URA4 gene
FT                   -> pJK210 5032bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA4 gene"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SacII-NotI-XbaI-SpeI-BamHI-SmaI-
FT                   PstI-EcoRI-EcoRV-HindIII-ClaI-SalI-HincII-XhoI-
FT                   ApaI-DraII-KpnI"
FT   misc_binding    0..0
FT                   /note="MCS unique SacI-SacII-NotI-XbaI-SpeI-BamHI-
FT                   SmaI-PstI-EcoRI-ClaI-SalI-HincII-XhoI-ApaI-DraII-KpnI"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli lac gene"
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SQ   Sequence 5032 BP; 1376 A; 1082 C; 1199 G; 1375 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accataagct tacaaatccc actggctata tgtatgcatt tgtgttaaaa aagtttgtat
     agattattta atctactcag cattctttct ctaaatagga atttgttact taatggagaa
     aaaaatgttt cgatttacct agtgtatttg tttgtatact cacgtttaat ttcaaacatc
     cattctatct tgtgtaattt ttggcatggt gaaaaagata atcagcctta taatctttac
     aaaagtaaga aattctgtaa ataagcctta atgcccttgc tttaaattaa aatggttctt
     tttcatgata atgtttgcac tttgtgaata tattttagat agttctgtga ggtataatta
     agatgtttta gagacttata caattttgtc tttataaatt cttaattgat tttaccatcc
     cagtttaact atgcttcgtc ggcatctctg cacatgtcgt gttttcttac cgtattgtcc
     taccaagaac ctcttttttg cttggatcga aattaaaggt ttaaaagcaa agttatggat
     gctagagtat ttcaaagcta ttcagctaga gctgagggga tgaaaaatcc cattgccaag
     gaattgttgg ctttgatgga agaaaagcaa agcaacttgt cagtcgcggt cgatttgacg
     aagaaatccg aaatcttaga attggtagat aaaattggac cctatgtctg tgttatcaag
     acacatattg acgttgtcga ggatttcgac caggatatgg tagaaaaact ggtggcctta
     ggtaaaaagc atcgttttct tatctttgag gatcgcaaat tcgcagacat tggaaatacc
     gtcaagctac aatatgcatc tggtgtgtac aaaattgctt cttgggctca tatcacaaat
     tgccatacag tgccaggcga gggtattata caaggcctca aagaagttgg tttacctttg
     ggacgtggtc tcttgctttt ggctgaaatg tcttccaaag gctctttggc tactggttcc
     tacacagaga aaaccttaga atggtttgag aagcataccg atttttgctt tggctttata
     gctggtcgtc gatttcctaa ccttcaaagc gactacataa ctatgtcccc tggtatcggc
     ttggatgtta aaggagacgg gctgggacag caatatcgta ctcctgaaga agtgattgta
     aactgcggta gcgatatcat cattgttggt cgtggagtct atggagctgg tcgtaatcct
     gttgtcgaag ccaagagata tagagaagct ggttggaagg catatcagca aagactttct
     cagcattaaa aaaagactaa tgtaaaattt ttttggttgg ttattgaaaa agtcgatgcc
     ttgtttgcgt ttgttttcct aggcgtttta tgtcagaagg catttagaat tagtatacaa
     gtactctttg gtaaaatttt atgtagcgac taaaatatta actattatag ataaacacct
     tgggaataaa aagtaatttg ctatagtaat ttattaaaca tgctcctaca acattaccac
     aatcttttct cttggattga cattgaataa gaaaagagtg aattttttta gacttgtaat
     gataactatg tacaaagcca atgaaagatg tatgtagatg aatgtaaaat accatgtaga
     caaacaagat aaaacttggt tataaacatt ggtgttggaa cagaataaat tagatgtcaa
     aaagtttcgt caatatcaca agcttatgcg gtgtgaaata ccgcacagat gcgtaaggag
     aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat
     tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
     aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
     agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata
     aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca
     ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctcggccc ccctgacgag
     catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     caggcgttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     cgttcatcca tagttgcctg actgcccgtc gtgtagataa ctacgatacg ggagggctta
     ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//