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ID   PJKK31     preliminary; circular DNA; SYN; 7400 BP.
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AC   ATCC37220;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/E.coli plasmid vector pJKK3-1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJC74 hly-1 from pJC74 & B.cereus GP4
RC   pJKK3-1 hly-1, pJKK3-1 hly-2 from pJKK3-1 & pJC74 hly-1
RA   Kreft J., Berger H., Hartlein M., Muller B., Weidinger G., Goebel W.;
RT   "Cloning and expression in Escherichia coli and Bacillus subtilis
RT   of the hemolysin (cereolysin) determinant from Bacillus cereus";
RL   J. Bacteriol. 155:681-689(1983).
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RN   [2]
RC   pBS1, pLS28, pBC16, pAB124, pAB128 from Bacillus
RC   pBC16-1, pBC161, pBC161-1 from pBC16
RC   RSF2124-B from RSF2124
RC   pLS101, pLS102, pLS103 from pLS28 & RSF2124-B
RC   pLS107 from pLS103
RC   pLS202, pLS206 from pLS103 & pBR322
RC   pLS207, pLS209 from pLS107 & pBR322
RC   pLL10 from pLS103
RC   [pBR322-Tmp from pBR322 & Tmp gene]
RC   pTL10 from pLL10 & pBR322-Tmp
RC   pTL11-3 from pTL10
RC   pTL12 from pTL11-3
RC   pAB224, pAB524 from pAB124
RC   pJK310 from pUB110 & pBR325
RC   pJK201 from pBS161-1 & pACYC184
RC   pJK312, pJK321, pJK521 from pJK series
RC   pJK523 from pC221 & pBR322
RC   pJK3 from pBR322 & pBS161-1
RC   pJK3-1, pJKK3-1 from pJK3
RC   pJK302 from pBR322 & pBC16-1
RC   pJK501 from pBR322 & pBS1
RC   pJK502 from pJK501
RC   pLK103 from pHC79 & pJK3
RC   pKJB200 from pBS161-1 & M13mp2
RC   pJK401 from pSC122 & pJK3-1
RC   pJK353 from pSB53 & pJK3-1
RC   pYearg4402-11 from Bacillus
RC   pJK3-1arg4 from pYearg4402-11 & pJK3-1
RA   Kreft J., Hughes C.;
RT   "Cloning vectors derived from plasmids and phage of Bacillus";
RL   Curr. Top. Microbiol. Immunol. 96:1-17(1981).
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RN   [3]
RC   pLS15 or pTA1010 from B.natto, no marker
RC   pLS16 from B.natto, no marker
RC   pLS17 from B.natto, no marker
RC   pLS18 from B.natto, no marker
RC   pLS19 from B.natto, no marker
RC   pLS20 from B.natto, no marker
RC   pLS21 from B.natto, no marker
RC   pLS22 from B.natto, no marker
RC   pLS23 from B.natto, no marker
RC   pLS24 from B.natto, no marker
RC   pLS25 from B.natto, no marker
RC   pLS26 from B.natto, no marker
RC   pLS27 from B.natto, no marker
RC   pLS28 from B.natto, no marker
RC   pLS29 from B.natto, no marker
RC   pLS30 from B.natto, no marker
RC   pLS31 from B.natto, no marker
RC   pLS32 from B.natto, no marker
RC   from pLS28 & B.subtilis leu gene
RA   Tanaka T., Koshikawa T.;
RT   "Isolation and charcaterization of four types of plasmids from
RT   Bacillus subtilis (natto)";
RL   J. Bacteriol. 131:699-701(1977).
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RN   [4]
RC   pAB118A, pAB118B from Bacillus strep gene
RC   pAB124 from Bacillus tet gene
RC   pAB128 from Bacillus tet gene
RA   Bingham A.H.A., Bruton C.J., Atkinson T.;
RT   "Isolation and partial characterization of four plasmids from
RT   antibiotic-resistant thermophilic bacilli";
RL   J. Gen. Microbiol. 114:401-408(1979).
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RN   [5]
RC   RSF2124-B-leu from RSF2124 & Bacillus leucine B gene
RC   RSF1010-B-leu from RSF2124-B-leu & RSF1010
RA   Nagahari K., Sakaguchi K.;
RT   "Cloning of Bacillus subtilis leucine A, B and C genes with
RT   Escherichia coli plasmids and expression of the leuC gene in E. coli";
RL   Mol. Gen. Genet. 158:263-270(1978).
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RN   [6]
RC   pSB53 from phoA gene
RA   Boidol W., Simonis M., Topert M., Siewert G.;
RT   "Recombinant plasmids with genes for the biosynthesis of alkaline
RT   phosphatase of Escherichia coli";
RL   Mol. Gen. Genet. 185:510-512(1982).
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RN   [7]
RC   pBC16-1, pBC16-2 from pBC16
RC   pBS161, pBS162 from pBC16-1 & pBS1
RC   pBS161-1 from pBS161
RC   pJK1, pJK2, pJK3, pJK4 from pBR322 & pBS161
RC   pJK101 from pACYC184 & pBS161
RC   from pWL7 & pBS161
RA   Kreft J., Bernhard K., Goebel W.;
RT   "Recombinant plasmids capable of replication in B. subtilis";
RL   Mol. Gen. Genet. 162:59-67(1978).
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RN   [8]
RC   RSF2124B.leu from RSF2124 & B.subtilis 168 leu gene
RC   pLS101, pLS102, pLS103 from pLS28 & RSF2124B.leu
RC   pLS107 from pLS103
RC   pLS202, pLS206 from pLS103 & pBR322
RC   pLS207, pLS208, pLS209 from pLS107 & pBR322
RA   Tanaka T., Sakaguchi K.;
RT   "Construction of a recombinant plasmid composed of B. subtilis
RT   leucine genes and a B. subtilis (natto) plasmid: its use as cloning
RT   vehicle in B. subtilis 168";
RL   Mol. Gen. Genet. 165:269-276(1978).
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RN   [9]
RC   pAB224 from pAB124
RC   pAB524 from pAB124
RA   Bingham A.H.A., Bruton C.J., Atkinson T.;
RT   "Characterization of Bacillus stearothermophilus plasmid pAB124
RT   and construction of deletion variants";
RL   J. Gen. Microbiol. 119:109-115(1980).
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RN   [10]
RC   pJK310 from pUB110 & pBR325
RC   pJK201, pJK202 from pBS161-1 & pACYC184
RC   pJK312 from pJK302 & pUB112
RC   pJK321 from pJK3-1 & pC221
RC   pJK521 from pJK series
RC   pJK523 from pC221 & pBR322
rc   pJK3 from pBR322 & pBS161-1
RC   pJK3-1, pJKK3-1 from pJK3
RC   pJK302 from pBR322 & pBC16-1
RC   pJK501 from pBR322 & pBS1
RC   pJK502 from pJK501
RC   pLK103 from pHC79 & pJK3
RC   pKJB200 from pBS161-1 & M13mp2
RC   pJK401 from pSC122 & pJK3-1
RC   pJK353 from pSB53 & pJK3-1
RC   pJK3-1arg4 from pYearg4402-11 & pJK3-1
RA   Goebel W., Kreft J., Burger K.J.;
RT   "Molecular cloning in Bacillus subtilis";
RL   Plasmids of Medical Environmental & Commercial Import 0:471-480(1979).
RL   Timmis K.N., Puhler A., eds., Elsevier/North Holland, Amsterdam.
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RN   [11]
RC   pUB1654 from pAB124
RA   Docherty A.;
RT   ;
RL   Unpublished (1980).
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CC   A Bacillus subtilis/E.coli shuttle vector.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pJKK3-1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli 5K)(Bacillus subtilis)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJK3 from pBR322 and pBS161-1, from pBC16)
CC   BR ()
CC   OF (pJK401)(pJK353)(pJK3-1arg4)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Bacillus subtilis
FT                   -> pBS1 7800bp [unique EcoRI]
FT                   1. pBC16 EcoRI-EcoRI 2700bp, tet gene
FT                   2. pBS1 EcoRI 7800bp
FT                   -> plasmid 10500bp
FT                   1. plasmid remove 2100bp, pBS1
FT                   -> pBS161 8200bp
FT                   1. pBS161 largest HindIII-HindIII 3000bp, tet gene
FT                   -> pBS161-1 3000bp [unique HindIII, EcoRI, PstI]
FT                   1. pBS161-1 HindIII 3000bp
FT                   2. pBR322 HindIII 4361bp 30..30
FT                   -> pJK3 7400bp
FT                   1. pJK3 PstI 7400bp 3614..3614
FT                   fill in
FT                   PstI, pBS1
FT                   fill in
FT                   -> pJKK3-1 7400bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pBC16"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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