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ID   PJL3       preliminary; circular DNA; SYN; 8300 BP.
AC   IG3000;
DT   07-SEP-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pJL3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJL from pTE103 & pRL25C
RC   pJL1 from pJL & cat gene & linkers
RC   pJL2 from pJL1 & pTLXT-11, rRNA gene terminator T1
RC   pJL3 from pJL2
RA   Lang J.D., Haselkorn R.;
RT   "A vector for analysis of promoters in the cyanobacterium Anabaena
RT   sp. strain PCC 7120";
RL   J. Bacteriol. 173:2729-2731(1991).
RN   [2]
RC   pTE100 from pUC8 & pLA4-TL, T7 terminator
RC   pTE102 from pTE100
RC   pTE103 from pTE102
RC   [pLA44 deltaH1 from pLA4]
RC   plasmid from pUC8CM & pLA44 deltaH1
RC   pTE110 from pTE103 & plasmid
RC   pTE112 from pTE110 & pTE103
RC   pTE114 from pTE112 & pTE103
RC   pTE122, pTE123 from pBR322 & pTE114
RA   Elliott T.E., Geiduschek E.P.;
RT   "Defining a bacteriophage late promoter: absence of a -35 region";
RL   Cell 36:211-219(1984).
RN   [3]
RC   pRL443 from RP4
RC   pRL449 from pACYC184 & Eco4711 methylase gene
RC   ColK from E.coli
RC   pRL518 from ColK & Eco4711 methylase gene
RC   pRL528 from ColK & Anabaena PCC7118 AvaI/Eco4711 methylase gene
RC   pRL530 from ColK & AvaI methylase gene
RC   pRL542 from ColK
RC   pRL25C from pBR322 & pDU1
RC   pJCF62 from F.diplosiphon
RC   16C12AK from Anabaena ATCC29413
RC   pSG111 from pBR322 & Synechococcus R2
RC   pSG111M from pSG111
RC   pUC303 from pACYC184
RC   pLF8 from pACYC177
RA   Elhai J., Wolk C.P.;
RT   "Conjugal transfer of DNA to cyanobacteria";
RL   Meth. Enzymol. 167:747-754(1988).
RN   [4]
RC   pRL7 from pRL1 & neo gene
RC   pRL7delta from pRL7
RC   pRL1VB from pRL1V
RC   pRL25 from pRL7delta & pRL1VB
RC   pRL25C from pRL25 & pWH5, cos
RA   Wolk C.P., Cai Y., Cardemil L., Flores E., Hohn B., Murry M.,
RA   Schmetterer G., Schrautemeier B., Wilson R.;
RT   "Isolation and complementation of mutants of Anabaena sp strain
RT   PCC 7120 unable to grow aerobically on dinitrogen";
RL   J. Bacteriol. 170:1239-1244(1988).
RN   [5]
RC   pTLXT-11 from rRNA gene terminator T1
RA   Deneer H., Spiegelman G.;
RT   ;
RL   Unpublished (1991).
RN   [6]
RC   pVW1 from pBR322 & pDU1, cyanobacteria
RC   pVW1C from pBR328 & pVW1
RC   pRL1 from pVW1C
RC   pRL5 from pRL1 & R300B
RC   ColE1::Tn5 from ColE1 & Tn5
RC   pRL6 from pRL1 & ColE1::Tn5
RC   pRL8 from pRL1 & pE194
RC   pRL1V from pRL1
RC   pRL5V from pRL5
RC   pRL6V from pRL6
RA   Wolk C.P., Vonshak A., Kehoe P., Elhai J.;
RT   "Construction of shuttle vectors capable of conjugative transfer
RT   from Escherichia coli to nitrogen-fixing filamentous cyanobacteria";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:1561-1565(1984).
RN   [7]
RC   pWH4, pWH5 from pDU1 & lambda, cos
RC   NOTE: see J. Bacteriol. 162:858-858(1985).
RA   Herrero A., Elhai J., Hohn B., Wolk C.P.;
RT   "Infrequent cleavage of cloned Anabaena variabilis DNA by
RT   restriction endonucleases from A. variabilis";
RL   J. Bacteriol. 160:781-784(1984).
RN   [8]
RC   pLA1 from pBR322 & phage T4 gene 23
RC   pLA3 from pBR322 & phage T4 gene 23
RC   pLA4 from pLA1
RC   pLA5 from pLA1
RA   Jacobs K.A., Albright L.M., Shibata D.K., Geiduschek E.P.;
RT   "Genetic complementation by cloned bacteriophage T4 late genes";
RL   J. Virol. 39:31-45(1981).
RN   [9]
RC   pA67 from pACYC184 & pLA1
RC   pG6B from pA67
RC   pGHA1 from pA67
RC   pGHA2 from pA67
RC   pG2AP-13 from pA67
RC   pA83 from pACYC184 & phage T4 Dec8
RA   Champness W.C., Snyder L.;
RT   "Bacteriophage T4 gol site: sequence analysis and effects of the
RT   site on plasmid transformation";
RL   J. Virol. 50:555-562(1984).
RN   [10]
RC   pDU1 from Nostoc PCC 7524 or Anabaena sp.
RA   Reaston J., Van Den Hondel C.A., Van Arkel G.A., Stewart W.D.;
RT   "A physical map of plasmid pDU1 from the cyanobacterium Nostoc PCC
RT   7524";
RL   Plasmid 7:101-104(1982).
RN   [11]
RC   pLA4 deltaH from pLA4
RC   pLA4-TL from pLA4 deltaH & T7 terminator
RA   Kassavetis G.A., Elliott T., Rabussay D.P., Geiduschek E.P.;
RT   "Initiation of transcription at phage T4 late promoters with
RT   purified RNA polymerase";
RL   Cell 33:887-897(1983).
CC   psbB gene.
CC   bacteriocin release protein (BRP) activates phospholipase A of outer
CC   cell membrane to make membrane permeable.
CC   Amount of permeability is controlled by induction with IPTG.
CC   Full induction inhibited with Mg++.
CC   NM (pJL3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli recA+ phospholipase A+)
CC   CP ()
CC   FN (cloning)(expression)(secretion)
CC   SE ()
CC   PA (pACYC184)(pRL25C)(pTE103)(pTLXT-11)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pLA4
FT                   -> pLA4 deltaH
FT                   1. pLA4 deltaH HpaI
FT                   2. phage T7 HpaIQ-HpaIQ 446bp, early terminator
FT                   -> pLA4-TL
FT                   1. pUC8 HindIII
FT                   2. pLA4-TL HpaI-HpaI, T7 early terminator
FT                   HindIII linker:HindIII linker
FT                   HindIII-HindIII
FT                   -> pTE100
FT                   1. pTE100 remove PvuII-EcoRI
FT                   :Klenow, fill in
FT                   -> pTE102 [EcoRI]
FT                   1. pTE102 HindIII, 3' to terminator
FT                   Klenow, fill in
FT                   -> pTE103
FT                   1. Nostoc PCC 7524 or Anabaena sp., cyanobacteria
FT                   -> pDU1 6280bp
FT                   1. pBR322 EcoRV, complete
FT                   2. pDU1 EcoRV, complete
FT                   -> pVW1 10640bp
FT                   1. pBR328 Sau3AI-Sau3AI, cat gene
FT                   calf intestinal alkaline phosphatase:
FT                   2. pVW1 BamHI
FT                   -> pVW1C 11620bp
FT                   1. pVW1C AvaII-Sau96I
FT                   calf intestinal alkaline phosphatase:
FT                   -> pRL1 9260bp [no AvaI, no AvaII, no AvrII
FT                   1. pRL1
FT                   2. neo gene
FT                   -> pRL7
FT                   1. pRL7 remove MstI-MstI, cat gene
FT                   -> pRL7delta
FT                   1. pRL1, cyanobacteria
FT                   -> pRL1V
FT                   1. pRL1V remove XhoI-XhoI pBR322 3213..3235, oriV
FT                   -> pRL1VB [unique BamHI]
FT                   1. pRL7delta remove NdeI-EcoRI, oriV
FT                   2. pRL1VB NdeI-EcoRI 1274bp, BamHI
FT                   -> pRL25 [unique BamHI]
FT                   1. pDU1
FT                   2. lambda, cos
FT                   -> pWH5
FT                   1. pRL25 ClaI
FT                   2. pWH5 AccI-AccI 403bp, cos
FT                   -> pRL25C 10140bp
FT                   1. pTE103 EcoRI-PvuII 600bp, MCS/early T4 terminator
FT                   Klenow fill in
FT                   EcoRI linker:EcoRI linker
FT                   EcoRI-EcoRI
FT                   2. pRL25C EcoRI
FT                   -> pJL
FT                   1. pJL SalI
FT                   2. 800bp, cat gene with no promoter
FT                   Klenow fill in:Klenow fill in
FT                   SalI linker:SalI linker
FT                   SalI-SalI
FT                   -> pJL1
FT                   1. pJL1 SmaI
FT                   2. pTLXT-11 EcoRI-EcoRI 170bp, rRNA gene term T1
FT                   Klenow fill in:Klenow fill in
FT                   -> pJL2
FT                   1. pJL2 BamHI
FT                   Klenow fill in
FT                   -> pJL3"
FT   misc_binding    1..1
FT                   /note="SIT unique EcoRI"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli miniplasmid p15A"
FT   CDS             complement(0..0)
FT                   /note="REP E. coli lacI repressor gene"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli bacteriocin release protein gene
FT                   (BRP)"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacPO promoter"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lpp operator"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;