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ID   PJOE810    preliminary; circular DNA; SYN; 3762 BP.
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AC   X13068;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pJOE810 - incomplete, MCS.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-90
RC   plasmid from pIC20H
RC   pJOE810 from plasmid & pIJ702
RA   Altenbuchner J.;
RT   "A new E.coli cloning vector containing a melanin marker for
RT   insertion screening";
RL   Nucleic Acids Res. 16:8710-8710(1988).
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CC   melanin marker for insertion screening in E.coli.
CC   MCS. not original sequence. deletion.
CC   NCBI gi: 58126
CC   NM (pJOE810)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Streptomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC20H)(pIJ702)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIC20H remove 2 stop codons
FT                   remove KpnI-NdeI 258bp 277..535, lacZalpha/2464bp
FT                   -> plasmid 2464bp
FT                   1. plasmid remove small BclI-SphI ?..244, 2400bp
FT                   2. pIJ702 BclI-SphI 1300bp, mel/tyrosinase gene
FT                   -> pJOE810 3762bp"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   CDS             0..0
FT                   /note="GEN Streptomyces ORF438"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Streptomyces"
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SQ   Sequence 90 BP; 22 A; 26 C; 23 G; 19 T; 0 other;
     atgaccatga ttacgccaac gttgcatgcc tgcaggtcga tcgacggatc cccgggaatt
     catcgatatc tagatccccg ggtaccggaa
//