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ID   PJOSP6SELF preliminary; circular DNA; SYN; 73 BP.
AC   IG5150;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pJOSP6-self - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pJMSP64 from pJMSP-8 & pSP64
RC   pB3-19-self from STobRV self-cleavage sequence
RC   pJOSP6-self from pJMSP64 & pB3-19-self, STobRV self-cleavage sequence
RA   Dzianott A.M., Bujarski J.J.;
RT   "An in vitro transcription vector which generates nearly correctly
RT   ended RNAs by self-cleavage of longer transcripts";
RL   Nucleic Acids Res. 16:10940-10940(1988).
RN   [2]
RC   pB3-17-self from pB3TP-8 & STobRV self-cleavage sequence
RC   pB3-19-self from pB3TP-8 & STobRV self-cleavage sequence
RC   pB1-4-self from pB1TP-7 & pB3-17-self
RC   pB2-12-self from pB2TP-3 & pB3-17-self
RA   Dzianott A.M., Bujarski J.J.;
RT   "Derivation of an infectious viral RNA by autolytic cleavage of in
RT   vitro transcribed viral cDNAs";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:4823-4827(1989).
RN   [3]
RC   pB1TP-7 from BMV
RC   pB2TP-3 from BMV
RC   pB3TP-8 from BMV
RA   Janda M., French R., Ahlquist P.;
RT   ;
RL   Virology 158:259-262(1987).
CC   MCS plus self-processing sequence. not original sequence.
CC   NM (pJOSP6-self)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pB3-19-self)(pJMSP64 from pSP64 & pJMSP-8)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pJMSP-8
FT                   2. pSP64
FT                   -> pJMSP64
FT                   1. BMV, RNA3
FT                   -> pB3TP-8
FT                   1. pB3TP-8 EcoRI, 3' to BMV RNA3
FT                   2. STobRV EcoRI-EcoRI 67bp, self-cleavage sequence
FT                   -> pB3-19-self
FT                   1. pJMSP64 remove small PvuII-EcoRI
FT                   2. pB3-19-self PvuII-EcoRI, STobRV self-cleavage seq
FT                   -> pJOSP6-self"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 73 BP; 17 A; 20 C; 19 G; 17 T; 0 other;
     atttaggtga cactataggc ctgcaggtcg actctagagg atccccgggt accgagctcg
     aattcgatac cct