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ID   PJQ200UC1  preliminary; circular DNA; SYN; 5900 BP.
AC   IG8075; ATCC77487;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli plasmid vector pJQ200uc1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pACYC184-Gm from pACYC184 & pPH1JI, gentamycin
RC   pJQ51.2 from pACYC184-Gm
RC   pJQ184 from pJQ51.2 & pHP45
RC   pEM5 from RP4, traJ & oriT & mob
RC   pJQ185 from pBluescript II KS & pEM5
RC   pJQ188 from pJQ185 & pUM24, sacB gene
RC   pJQ199 from pJQ184 & pJQ188
RC   pJQ200 from pJQ199
RC   pJQ210 from pJQ199 & pHC79, lambda cos
RC   pJQ254 from pK18 & oligo
RA   Quandt J., Hynes M.F.;
RT   "Versatile suicide vectors which allow direct selection for gene
RT   replacement in gram-negative bacteria";
RL   Gene 127:15-21(1993).
CC   Suicide vector permitting gene replacement and mobilization into a
CC   wide range of Gram negative bacteria.  Allows positive selection for
CC   integration. Replicates only in enterobacteria, and so functions as a
CC   suicide vector in other Gram negative hosts. When induced by media
CC   containing 5% sucrose, sacB is lethal in a wide range of Gram negative
CC   bacteria, and thus permits selection for loss of the vector.
CC   A single BglII site within the gentamicin resistance gene (gtmR)
CC   allows insertion of other resistance genes (e.g., as a BamHI
CC   cassette).
CC   Common restriction sites are minimized to allow use of sites within
CC   the insert to create insertions, deletions, frameshifts and base
CC   replacements.
CC   The order of the major features of the plasmid is:  gtmR -
CC   NotI/SmaI/NotI - P15A - traJ - oriT - sacB. [1]
CC   Deposited by: M.F.Hynes
CC   Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C
CC   NM (pJQ200uc1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(integrating)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pJQ200 SmaI 4900bp
FT                   2. pJQ254 PvuII-PvuII 605bp, MCS
FT                   -> pJQ200uc1 5900bp"
FT   misc_binding    0..0
FT                   /note="MCS NotI SmaI NotI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli p15A oriV"
FT   rep_origin      0..0
FT                   /note="ORI E. coli oriT"
FT   CDS             0..0
FT                   /note="GEN E. coli traJ gene"
FT   CDS             0..0
FT                   /note="GEN E. coli gtmR gene"
FT   CDS             0..0
FT                   /note="GEN E. coli sacB gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;