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ID   PJQ210MP18 preliminary; circular DNA; SYN; 7350 BP.
AC   IG8078; ATCC77491;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli cosmid vector pJQ210mp18 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pACYC184-Gm from pACYC184 & pPH1JI, gentamycin
RC   pJQ51.2 from pACYC184-Gm
RC   pJQ184 from pJQ51.2 & pHP45
RC   pEM5 from RP4, traJ & oriT & mob
RC   pJQ185 from pBluescript II KS & pEM5
RC   pJQ188 from pJQ185 & pUM24, sacB gene
RC   pJQ199 from pJQ184 & pJQ188
RC   pJQ200 from pJQ199
RC   pJQ210 from pJQ199 & pHC79, lambda cos
RC   pJQ254 from pK18 & oligo
RA   Quandt J., Hynes M.F.;
RT   "Versatile suicide vectors which allow direct selection for gene
RT   replacement in gram-negative bacteria";
RL   Gene 127:15-21(1993).
CC   Suicide vector permitting gene replacement and mobilization into a
CC   wide range of Gram negative bacteria.  Allows positive selection for
CC   integration. Replicates only in enterobacteria, and so functions as a
CC   suicide vector in other Gram negative hosts. When induced by media
CC   containing 5% sucrose, sacB is lethal in a wide range of Gram negative
CC   bacteria, and thus permits selection for loss of the vector.
CC   A single BglII site within the gentamicin resistance gene (gtmR)
CC   allows insertion of other resistance genes (e.g., as a BamHI
CC   cassette).
CC   The order of the major features of the plasmid is:  gtmR -
CC   EcoRI/MCS/HindIII - P15A - cos - traJ - oriT - sacB. [1]
CC   Deposited by: M.F.Hynes
CC   Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C
CC   NM (pJQ210mp18)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(integrating)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pJQ210 SmaI 6700bp
FT                   2. pK18 PvuII-PvuII 633bp 2268..2661..240, MCS [632bp]
FT                   -> pJQ210mp18 7350bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-KpnI-RsaI-SmaI-BamHI-XbaI-
FT                   SalI-PstI-SphI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli p15A oriV"
FT   rep_origin      0..0
FT                   /note="ORI E. coli oriT"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
FT   CDS             0..0
FT                   /note="GEN E. coli gtmR gene"
FT   CDS             0..0
FT                   /note="GEN E. coli sacB gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;