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ID   PJRD215    preliminary; circular DNA; SYN; 10200 BP.
XX
AC   M16198; ATCC37533;
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DT   02-APR-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli cosmid vector pJRD215 - KmR-cos region.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2549
RC   pJRD185 from pJRD184
RC   pJRD186 from pIC20R & pJRD185
RC   pEMBLcos from pEMBL8 & MUA-10, lambda cos
RC   RSF1010KmR or RSF1010Kan from RSF1010 & pKC7
RC   pJRD203 from RSF1010KmR or RSF1010Kan & pEMBLcos
RC   pJRD204 from pJRD203
RC   pJRD209 from pJRD203
RC   pJRD210 from pJRD204
RC   pJRD212 from pJRD209 & pJRD210
RC   pJRD215 from pJRD212 & pJRD186
RA   Davison J., Heusterspreute M., Chevalier N., Ha-Thi V., Brunel F.;
RT   "Vectors with restriction site banks: V. pPJRD215, a wide-host-
RT   range cosmid vector with multiple cloning sites";
RL   Gene 51:275-280(1987).
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RN   [2]
RC   pJRD215
RA   Davison J.;
RT   ;
RL   Submitted (01-JUL-1987) to GenBank on computer-readable media by:
RL   Davison J., .
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CC   Broad host range, multiple cloning site cosmid that can be
CC   transferred by conjugation.
CC   Medium is 1236 LB plus kanamycin.
CC   NCBI gi: 208692
CC   NM (pJRD215)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(E.coli)(broad host range)
CC   CP ()
CC   FN (cloning)(shuttle wide-host-range)(restriction site bank)
CC   SE ()
CC   PA (pBR327)(lambda)(pEMBL8)(MUA-10)(pJRD186)(pBR322)(Tn5m)
CC   BR (pJRD158)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
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FT   misc_feature    0..0
FT                   /note="1. pJRD184 remove small BclI-BglII 5bp
FT                   \ 1605..1610, 3898bp
FT                   -> pJRD185 3898bp
FT                   1. pIC20R HindIII-KpnI 43bp 264..307, MCS
FT                   2. pJRD185 remove HindIII-KpnI 1298bp 30..1328, 2600bp
FT                   -> pJRD186 2600bp
FT                   1. pEMBL8+ PstI 3939bp 255..255
FT                   2. MUA-10 PstI-PstI 403bp, lambda cos
FT                   -> pEMBLcos 4342bp
FT                   1. RSF1010 remove EcoRI-PstI 904bp 7773..8677,
FT                   \ sulf/7780bp
FT                   2. pKC7 EcoRI-PstI 1447bp 5834..5835..1446, Tn5 kan
FT                   -> RSF1010KmR 9227bp
FT                   1. RSF1010KmR remove small EcoRI-HindIII 8677..?
FT                   2. pEMBLcos EcoRI-HindIII 433bp,
FT                   \ pEMBL8+ 229..-..259 MCS/lambda cos
FT                   -> pJRD203 9100bp
FT                   \ [unique BglII-HindIII-SalI-BamHI-EcoRI-HpaI-SacI]
FT                   1. pJRD203 remove small EcoRI-HpaI, 9100bp
FT                   2. pBR322 EcoRI-EcoRV 189bp 4360..4361..188,
FT                   \ tet promoter/185bp
FT                   -> pJRD204 9000bp [unique ClaI, no HpaI, 2 HindIII]
FT                   1. pJRD203 HindIII 9100bp
FT                   fill in
FT                   -> pJRD209 9100bp [unique NheI]
FT                   1. pJRD204 AsuII 9000bp
FT                   Klenow
FT                   XhoI linker 6bp ctcgag
FT                   -> pJRD210 9000bp [unique XhoI]
FT                   1. pJRD209 9100bp BglII-SalI
FT                   2. pJRD210 remove BglII-SalI
FT                   -> pJRD212 9000bp [unique XhoI, NheI, HindIII]
FT                   1. pJRD212 SmaI-BstEII
FT                   2. pJRD212 BstEII-BamHI
FT                   3. pJRD186 BamHI-PvuII 432bp, MCS
FT                   -> pJRD215 10200bp"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan);
FT                   fragment; from pKC7"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str);
FT                   fragment; from pKC7"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos region"
FT   misc_recomb     1467..1468
FT                   /note="Tn5 DNA end/pEMBL8 DNA start"
FT   misc_recomb     1483..1484
FT                   /note="pEMBL8 DNA end/MuA-10 DNA start"
FT   misc_recomb     1915..1916
FT                   /note="MuA-10 DNA end/pEMBL8 DNA start"
FT   misc_recomb     1923..1924
FT                   /note="pEMBL8 DNA end/pJRD186 DNA start"
FT   misc_recomb     2357..2358
FT                   /note="pJRD186 DNA end/pEMBL8 DNA start"
FT   misc_recomb     2360..2361
FT                   /note="pEMBL8 DNA end/pBR322 DNA start"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-KpnI-AsuII-MluI-HpaI-ApaI-NsiI-
FT                   NdeI-StuI-XbaI-SnaBI-SpeI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT   misc_binding    0..0
FT                   /note="SIT BstEII"
FT   misc_binding    0..0
FT                   /note="SIT MstII"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   misc_binding    0..0
FT                   /note="SIT DraI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
XX
SQ   Sequence 2549 BP; 607 A; 725 C; 655 G; 562 T; 0 other;
     ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga
     tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact
     cgcgagggga tcgagcccgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgcctcg
     aggcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
     tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
     ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg
     gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt
     gcgccctgag tgcttgcggc agcgtgaagc tagcttggct gcaggggggg gggggggact
     tccattgttc attccacgga caaaaacaga gaaaggaaac gacagaggcc aaaaagcctc
     gctttcagca cctgtcgttt cctttctttt cagagggtat tttaaataaa aacattaagt
     tatgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata gcgaaaaccc
     gcgaggtcgc cgccccgtaa cctgtcggat caccggaaag gacccgtaaa gtgataatga
     ttatcatcta catatcacaa cgtgcgtgga ggccatcaaa ccacgtcaaa taatcaatta
     tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaaacaa cttcagacaa
     tacaaatcag cgacactgaa tacggggcaa cctcatgtcc cccccccccc ctgcaggtcg
     acggatcccc gggtaccata agtagaagca gcaacccaag tagctttacc agcatccggt
     tcaaccagca tagtaagaat cttactggac atcggcagtt cttcgaacag tgcgccaact
     accagctctt tctgcagttc attcagggca ccggagaacc tgcgtgcaat ccatcttgtt
     caatcatgcg aaacgatcct catcctgtct cttgatccat ggattacgcg ttaacccggg
     cccgcggatg catatgatct taaggcctag gtctagagtc tttgttttga cgccattagc
     gtacgtaaca atcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac
     ggagtatact agtatagtct atagtccgtg gaattattat atttatctcc gacgatattc
     tcatcagtga aatccagggg aattctcatg tttgacagct tatcatcgat aagctttaat
     gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca
     atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta
     tgccggtact gccgggcctc ttgcgggat
//