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ID   PKBY2      preliminary; circular DNA; SYN; 9900 BP.
AC   ATCC39898;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Aspergillus/E.coli cosmid vector pKBY2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pKBY0.1 from pJB8 & pBR329
RC   pKBY0.2 from pKBY0.1 & A.nidulans trpC gene
RC   pKBY1 from pKBY0.2 & linker
RC   pKBY2 from pKBY1
RA   Yelton M.M., Timberlake W.E., Van Den Hondel C.A.;
RT   "A cosmid for selecting genes by complementation on Aspergillus
RT   nodulans - se;lection of the developmentally regulated yA locus";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:834-838(1985).
CC   The BamHI site was removed from trpC by sodium bisulfite mutagenesis.
CC   A 2.1 kb HindIII/SalI fragment (pJB8) containing lambda cos was
CC   ligated to HindIII/SalI-digested pBR329. A 4.1 kb XhoI fragment with
CC   wild-type Aspergillus nidulans trpC was ligated into the SalI, and a
CC   BamHI linker into the NruI site of pBR329.
CC   This is a cosmid vector for isolating genes by complementation of
CC   mutations in Aspergillus nidulans. It can also be used for library
CC   construction.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKBY2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(Aspergillus nidulans)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pJB8
FT                   1. pJB8 HindIII-SalI 2100bp, lambda cos
FT                   2. pBR329 remove small HindIII-SalI
FT                   -> pKBY0.1
FT                   1. pKBY0.1 SalI
FT                   2. A.nidulans XhoI-XhoI 4100bp, trpC gene
FT                   -> pKBY0.2
FT                   1. pKBY0.2 NruI, pBR329 part
FT                   BamHI linker
FT                   -> pKBY1
FT                   1. pKBY1 BamHI, trpC gene
FT                   mutagenesis
FT                   -> pKBY2 9900bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="GEN E. coli trpC+ gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;