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ID   PKC1064    preliminary; circular DNA; SYN; 10000 BP.
AC   IG8001;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Streptomyces/E.coli phagemid vector pKC1064 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pKC293 from pBR322 & pIJ361 & pFJ103 & pKC7 & pTI104
RC   pKC305 from pBR322 & pACYC184 & Tn5 & Charon 4A
RA   Kuhstoss S., Rao R.N.;
RT   "Expression in Streptomyces ambofaciens of an Escherichia coli
RT   K-12 gene which confers resistance to hygromycin B";
RL   Gene 26:295-299(1983).
RN   [2]
RC   pHJL200, pHJL201, pHJL202, pHJL203, pHJL204, pHJL205 from pHJL192
RC   pHJL210, pHJL211 from pHJL201 & pIJ702
RC   pHJL8 from pHJL197 & pKC7
RC   pHJL10 from pHJL8
RC   pHJL220, pHJL221, pHJL222, pHJL223 from pHJL10 & pHJL125
RC   pHJL224, pHJL225 from pHJL10 & pHJL125
RC   pHJL399 from pHJL225 & tsr gene
RC   pHJL400, pHJL401 from pHJL399 & pUC19
RC   pHJL236 from pHJL224 & pHJL10
RC   pHJL241 from pHJL236 & pHJL196
RC   pHJL250 from pHJL241 & tsr gene
RC   pHJL251 from pHJL250
RC   pHJL302 from pHJL251 & pUC19
RC   [pHJL366 from SCP2*, Streptomyces ori]
RA   Larson J.L., Hershberger C.L.;
RT   "The minimal replicon of a streptomycete plasmid produces an
RT   ultrahigh level of plasmid DNA";
RL   Plasmid 15:199-209(1986).
RN   [3]
RC   pKC203 from E. coli, hyg B gene
RC   pKC222 from pKC7 & pKC203, hyg B gene
RC   pKC237 from pKC222
RC   pTI104 from pKC222 & linker
RC   pTI105 from pTI104 & lambda, pL
RC   plasmid from pTI104 & pMC1403
RC   pKC307 from pUC8 & plasmid
RA   Kaster K.R., Burgett S.G., Rao R.N., Ingolia T.D.;
RT   "Analysis of a bacterial hygromycin B resistance gene by
RT   transcriptional and translational fusions and by DNA sequencing";
RL   Nucleic Acids Res. 11:6895-6911(1983).
RN   [4]
RC   pFJ103 from Streptomyces granuloruber
RC   plasmid from pBR322 & Streptomyces thiostrepton gene
RC   pFJ104 from pFJ103 & plasmid
RC   pFJ105 from pFJ103 & plasmid
RC   plasmid2 from pBR322 & neo gene
RC   pFJ123 from pFJ105 & plasmid2
RC   pFJ164 from pIJ6 & pBR322
RC   pFJ165 from pIJ2 & pBR322
RA   Richardson M.A., Mabe J.A., Beerman N.E., Nakatsukasa W.M.,
RA   Fayerman J.;
RT   "Development of cloning vehicles from the Streptomyces plasmid
RT   pFJ103";
RL   Gene 20:451-457(1982).
RN   [5]
RC   pKC418 from aac(3)IV gene
RC   pKC527 from pHJL302
RC   pKC541, pKC542, pKC543 from pKC527 & pSAM2
RC   pKC555 from pKC542 & aac(3)IV gene
RC   pKC608 from lambda, cos
RC   pKC623 from pKC608 & pSAM2
RC   pKC702 from pKC527 & pSAM2
RA   Kuhstoss S., Richardson M.A., Rao R.N.;
RT   "Site-specific integration in Streptomyces ambofaciens:
RT   localization of integration functions in S. ambofaciens plasmid
RT   pSAM2";
RL   J. Bacteriol. 171:16-23(1989).
RN   [6]
RC   pHJL125 from SCP2*, Streptomyces ori
RC   pHJL192 from pBR322 & Streptomyces aph gene & SCP2*, Streptomyces ori
RC   pHJL196 from SCP2*, Streptomyces ori
RC   pHJL197 from SCP2*, Streptomyces ori
RC   pHJL401 from SCP2*, Streptomyces ori
RA   Larson J.L., Hershberger C.L.;
RT   "Shuttle vectors for cloning recombinant DNA in Escherichia coli
RT   and Streptomyces griseofuscus C581";
RL   J. Bacteriol. 157:314-317(1984).
RN   [7]
RC   pSG1 from Streptomyces griseus
RA   Cohen A., Bar-Nir D., Goedeke M.E., Parag Y.;
RT   "The integrated and free states of Streptomyces griseus plasmid
RT   pSG1";
RL   Plasmid 13:41-50(1985).
RN   [8]
RC   pMEA100 from Nocardia mediterranei
RA   Madon J., Moretti P., Hutter R.;
RT   "Site-specific integration and excision of pMEA100 in Nocardia
RT   mediterranei";
RL   Mol. Gen. Genet. 209:257-264(1987).
RN   [9]
RC   SCP1 from S.coelicolor A3(2)
RA   Vivian A.;
RT   "Genetic control of fertility in Streptomyces coelicolor A3(2):
RT   plasmid involvement in the interconversion of UF and IF strains";
RL   J. Gen. Microbiol. 69:353-364(1971).
RN   [10]
RC   pSE101 from S. erythraeus
RA   Katz L., Brown D.P., Tuan J.S., Chiang S.D., Katz L.;
RT   "Site-specific integration in Saccharopolyspora erythraea and
RT   multisite integration in Streptomyces lividans of actinomycete
RT   plasmid pSE101";
RL   J. Bacteriol. 170:2287-2295(1988).
RN   [11]
RC   pOS7 [similar to pSAM2] from S. ambofaciens
RA   Simonet J.M., Boccard F., Perndoet J.L., Gagnat J., Guerineau M.;
RT   "Excision and integration of a self-transmissible replicon of
RT   Streptomyces ambofaciens";
RL   Gene 59:137-144(1987).
RN   [12]
RC   pKC473 from pKC505 & pBR322
RC   pKC731 from pIJ500 & pKC473
RC   pKC767 from pKC541 & pKC473
RC   pKC787 from pUC18
RC   pKC796 from pIJ500 & pKC787
RC   pKC824 from pKC541 & pKC787
RA   Kuhstoss S., Richardson M.A., Rao R.N.;
RT   "Plasmid cloning vectors that integrate site-specifically in
RT   streptomycetes";
RL   Gene 97:143-146(1991).
RN   [13]
RC   pKC488 from pKC462a & S.erythreus, ery gene
RC   pKC488 from pUC18 & SCP2* & RP4
RA   Stanzak R., Matsushima P., Baltz R.H., Rao R.N.;
RT   "Cloning and expression in Streptomyces lividans of clustered
RT   erythromycin biosynthesis genes from Streptomyces erythreus";
RL   Biotechnology 4:229-232(1986).
RN   [14]
RC   pKC31 from lambda dv series & Tn5
RC   pKC420 from pKC222 & pFJ103 & pTR262 & pcos4EMBL
RC   pKC505 from pKC222 & pHJL202 & pcos4EMBL
RC   pKC513 from pKC462a & pcos2EMBL
RC   pKC521 from pHC79 & pKC31
RC   pKC531 from pKC222 & pHJL125 & pcos4EMBL
RC   pKC561 from pIJ922 & pKC521
RC   pKC575 from lambda dv series & SCP2*
RA   Rao R.N., Richardson M.A., Kuhstoss S.A.;
RT   "Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA";
RL   Meth. Enzymol. 153:166-198(1987).
RN   [15]
RC   [pKC912delta16 from pKC series]
RC   pKC1064 from tipA promoter & pIJ486 & pHJL366
RA   Kuhstoss S., Rao R.N.;
RT   "A thiostrepton-inducible expression vector for use in Streptomyces
RT   spp";
RL   Gene 103:97-99(1991).
RN   [16]
RC   phiC31 from Streptomyces
RC   pIJ500 from phage KC100
RA   Chater K.F.;
RT   "Streptomyces phages and their applications to Streptomyces genetics";
RL   The Bacteria Treatise on Structure and Functions 0:119-158(1986).
RL   Queener S.W., Day L.E., eds., Academic Press, Orlando FL.
RN   [17]
RC   pKC462a from pFJ103
RA   Feiss M., Siegele D.A.;
RT   "Packaging of the bacteriophage lambda chromosome: dependence of
RT   cos cleavage on chromosome length";
RL   Virology 92:190-200(1979).
RN   [18]
RC   pIJ610 from pBR322 & pIJ101
RA   Kieser T.;
RT   ;
RL   Unpublished (1987).
CC   NM (pKC1064)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli K-12)(Streptomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIJ486)(pUC18)(pHJL366)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. SCP2*, Streptomyces ori
FT                   -> pHJL366
FT                   1. pIJ486
FT                   2. pHJL366, SCP2* Streptomyces ori
FT                   -> plasmid 10000bp
FT                   1. plasmid remove BglII-SmaI, MCS/10000bp
FT                   2. S.lividans tipA promoter BglII-SmaI 145bp 2..146
FT                   -> pKC1064 10000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RP4 oriT"
FT   CDS             0..0
FT                   /note="ANT Streptomyces thiostrepton gene"
FT   rep_origin      0..0
FT                   /note="ORI Streptomyces SCP2"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene alpha peptide"
FT   promoter        0..0
FT                   /note="PRO S.lividans tipA promoter"
FT   terminator      0..0
FT                   /note="TER phage fd"
SQ   Sequence 69 BP; 28 A; 7 C; 17 G; 17 T; 0 other;
     agatcttagt tgattgaagg aggaatttaa aatgtacagc atcaagacag gaggaatctg