ID   PKH502     preliminary; circular DNA; SYN; 4200 BP.
XX
AC   M12533; ATCC37293;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKH502 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMY30-2 from p344 & lambda dv021
RC   pMA001 from pMC1871 & pMY30-2
RC   pKH4 from pBR322 & pTM2
RC   pKH502 from pKH4
RC   pKH702 from pKH502 & pMA001
RA   Shirakawa M., Tsurimoto T., Matsubara K.;
RT   "Plasmid vectors designed for high-efficiency expression
RT   controlled by the portable recA promoter-operator of Escherichia
RT   coli";
RL   Gene 28:127-132(1984).
XX
CC   Expression vector regulated by nalidixic acid induction of the recA
CC   promoter.The 236 bp EcoRI/BamHI fragment can serve as a portable
CC   promoter.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKH502)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli Km723)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKH4 ClaI 4598bp
FT                   BAL31 nuclease
FT                   BamHI linker 6bp ggatcc
FT                   BamHI-BamHI
FT                   -> pKH502 4200bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO E. coli recA gene"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//