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ID   PLA2905    preliminary; circular DNA; SYN; 21000 BP.
AC   ATCC37354;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli cosmid vector pLA2905 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pLA2901 from pRK290 & pBR322
RC   pLA2905 from pLA2901
RC   pLA2910, pLA2917 from pLA2901 & pMF517
RA   Allen L.N., Hanson R.S.;
RT   "Construction of broad-host-range cosmid cloning vectors:
RT   identification of genes necessary for growth of Methylobacterium
RT   organophilum on methanol";
RL   J. Bacteriol. 161:955-962(1985).
RN   [2]
RC   pMO172 from R68.45
RA   Moore A.T., Nayudu M., Holloway B.W.;
RT   "Genetic mapping in Methylphilus methylotrophus AS1";
RL   J. Gen. Microbiol. 129:785-799(1983).
RN   [3]
RC   ColIa-CA53 from ColIa
RC   pJK1 from ColIa-CA53 & pBR322
RC   pJK5 from pJK1
RC   pKP37 from lambda & pJK5
RC   pAR1, pAR2 from pJK5
RC   pRA2 from pAR2
RC   pAR6, pAR29 from pBR322 & pAR2
RC   pAR7 from pAR6
RC   pCA6 from pAR6 & pBR322
RC   pCA33, pCA35 from lambda::Tn5 & pAR6
RA   Weaver C.A., Redborg A.H., Konisky J.;
RT   "Plasmid-determined immunity of Escherichia coli K-12 to colicin
RT   Ia is mediated by a plasmid-encoded membrane protein";
RL   J. Bacteriol. 148:817-828(1981).
RN   [4]
RC   from R68.45
RA   Tatra P.K., Goodwin P.M.;
RT   "R-plasmid-mediated chromosome mobilization in the facultative
RT   methylotroph Pseudomonas AM1";
RL   J. Gen. Microbiol. 129:2629-2632(1983).
RN   [5]
RC   lambda::Tn5 from Tn5 & lambda, cI857
RA   Baseford P.;
RT   ;
RL   Unpublished (1981).
RN   [6]
RC   R68.45 from Pseudomonas AM1
RA   Haas D., Holloway B.W.;
RT   "R-factor variants with enhanced sex factor activity in Pseudomonas
RT   aeruginosa";
RL   Mol. Gen. Genet. 144:243-251(1976).
CC   A broad host range plasmid vector.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pLA2905)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. RK2
FT                   -> pRK290 20000bp
FT                   1. pBR322 HindIII 4361bp 30..30
FT                   2. Tn5 XhoI-XhoI 2347bp 486..2833, kan gene
FT                   -> plasmid 6800bp
FT                   1. pRK290 BglII 20000bp
FT                   2. plasmid BamHI-BamHI 1663bp 1516..2833/30..376,
FT                   \ kan gene
FT                   -> pLA2901 22000bp [tet, kan]
FT                   1. pLA2901 XhoI 22000bp
FT                   BAL31 nuclease
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pLA2905 21000bp
FT                   \ [no SalI or PstI between tet and kan]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-HindIII-SalI-PstI-BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT Methylobacterium tetracycline resistance
FT                   gene (tet)"
FT   CDS             0..0
FT                   /note="ANT Methylobacterium kanamycin resistance gene
FT                   (kan)"
FT   rep_origin      0..0
FT                   /note="ORI Methylobacterium oriV"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;