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ID   PLA2917    preliminary; circular DNA; SYN; 21000 BP.
AC   ATCC37355; ATCC39840;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Broad host range/E.coli cosmid vector pLA2917 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pLA2901 from pRK290 & pBR322
RC   pLA2905 from pLA2901
RC   pLA2910, pLA2917 from pLA2901 & pMF517
RA   Allen L.N., Hanson R.S.;
RT   "Construction of broad-host-range cosmid cloning vectors:
RT   identification of genes necessary for growth of Methylobacterium
RT   organophilum on methanol";
RL   J. Bacteriol. 161:955-962(1985).
RN   [2]
RC   pLA2917 from pLA2901 & pMF517
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,824,786 dated Apr. 25, 1989.
CC   A broad host range plasmid vector.
CC   This is a cosmid with a broad host range that can be used to
CC   characterize the genome of C1-utilizing microorganisms. [2]
CC   This cosmid can be mobilized for transfer by conjugation between
CC   E.coli and other strains in the presence of helper plasmid
CC   pRK2013. [2]
CC   The BglII and PstI sites interrupt the kanamycin resistance gene; the
CC   SalI site interrupts the tetracycline resistance gene. [2]
CC   This vector transfers efficiently to a broad host range including
CC   E.coli HB101, Methylobacterium organophilum DSM 761, Pseudomonas
CC   AM1, Pseudomonas putida, and Pseudomonas aeruginosa. [2]
CC   DNA inserts, 25-40 kb in size, can be cloned into the BglII site and
CC   mobilized from E.coli to M. organophilum.(personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   PstI--21.0; HindIII--21.0; BglII--21.0; EcoRI--17.0, 4.0; (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pLA2917)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pLA2905 XhoI 21000bp
FT                   BAL31 nuclease
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pMF517 HindIII 7100bp, pBR322 30..30
FT                   BAL31 nuclease
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   PvuII-PvuII 400bp, lambda cos
FT                   -> pLA2917 21000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII-HpaI-HindIII-SalI-PstI-BglII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT Methylobacterium tetracycline resistance
FT                   gene (tet)"
FT   CDS             0..0
FT                   /note="ANT Methylobacterium kanamycin resistance gene
FT                   (kan)"
FT   rep_origin      0..0
FT                   /note="ORI Methylobacterium oriV"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;