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ID PLA2920 preliminary; circular DNA; SYN; 20000 BP.
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE Broad host range/E.coli cosmid vector pLA2920 - incomplete.
KW cloning vector.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC pLA2920 from pLA2917
RL Unpublished (1989).
RL U.S. Patent No. 4,824,786 dated Apr. 25, 1989.
CC This vector transfers efficiently to a broad host range including
CC E.coli HB101, Methylobacterium organophilum DSM 761, Pseudomonas
CC AM1, Pseudomonas putida, and Pseudomonas aeruginosa.
CC This was constructed from pLA2917 (ATCC 39840) by deleting the
CC kanamycin resistance segment from Tn5 to avoid internal rearrangement
CC when the transposon moves and to permit selection of conjugants that
CC have a Tn5 insertion.
CC This is a cosmid with a broad host range that can be used to
CC characterize the genome of C1-utilizing microorganisms.
CC Restriction digests of the clone give the following sizes (kb):
CC PstI--20.0; HindIII--20.0; BglII--20.0; EcoRI--17.0; 2.6. (ATCC staff)
CC Medium is 1273 LB plus tetracycline.
CC NM (pLA2920)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (ATCC)
CC HO (E.coli HB101)(broad host range)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. pLA2917 remove 1000bp, Tn5 kan gene/20000bp
FT -> pLA2920 20000bp"
FT misc_binding 0..0
FT /note="SIT unique SalI-BstEII"
FT rep_origin 0..0
FT /note="ORI Pseudomonas pLA2917 oriV"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;