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ID   PLC544     preliminary; circular DNA; SYN; 10500 BP.
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AC   ATCC37013;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pLC544 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pYe(TRP1)54 from ColE1 & yeast chromosome
RC   pYe(TRP1)74 from ColE1 & yeast chromosome
RC   pLC544, pLC548 from pBR313 & pYe(TRP1)54
RC   pYe(CDC10)1 from pLC544 & yeast chromosome III CDC10 gene
RC   pYe(CEN3)11 or pYe(CDC10)1-1 from pLC544 & pYe(CDC10)1
RC   pYe35 or pYe(CDC10)1-2 from pLC544 & pYe(CDC10)1
RA   Clarke L., Carbon J.;
RT   "Isolation of the centromere-linked CDC10 gene by complementation
RT   in yeast";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:2173-2177(1980).
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RN   [2]
RC   pYe98F4T from pLC544 & yeast chromosome III CDC10 gene
RC   pYe65H3T from pLC544 & yeast chromosome III CDC10 gene
RC   pYe101C3T from pLC544 & yeast chromosome III CDC10 gene
RC   pYe(CEN3)21 from pGT12 & pYe(CEN3)11
RC   pYe(CEN3)41 from pGT12 & pYe(CEN3)11
RC   pYe(ars2)26 from ARG4 gene & TRP5 gene & ARS2
RC   pYe(ars1-ars2)1 from pYe(ars2)26 & ARS1/TRP1 gene
RA   Clarke L., Carbon J.;
RT   "Isolation of a yeast centromere and construction of functional
RT   small circular chromosomes";
RL   Nature 287:504-509(1980).
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RN   [3]
RC   pLC544 from pBR313 & yeast TRP1 gene
RA   Kingsman A.J., Clarke L., Mortimer R.K., Carbon J.;
RT   "Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying
RT   DNA from the yeast TRP1 region";
RL   Gene 7:141-153(1979).
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RN   [4]
RC   pLC1-3, pLC42-22 from ColE1 & E. coli glyS/xyl gene
RC   pLC1-28, pLC1-29 from ColE1 & E. coli flaGH/cheB/mot gene
RC   pLC3-46, pLC8-12, pLC8-24, pLC8-29 from ColE1 & E. coli glpT gene
RC   pLC4-39, pLC8-9, pLC25-8, pLC30-24 from ColE1 & E. coli dnaC gene
RC   pLC31-39 from ColE1 & E. coli dnaC gene
RC   pLC11-9, pLC44-14 from ColE1 & E. coli dnaB gene
RC   pLC14-12, pLC19-24, pLC42-17 from ColE1 & E. coli glpT gene
RC   pLC4-6, pLC5-23, pLC29-41, pLC32-12 from ColE1 & E. coli trpE gene
RC   pLC32-27, pLC41-15 from ColE1 & E. coli trpE gene
RC   pLC5-1, pLC5-2, pLC6-2, pLC10-24 from ColE1 & E. coli dnaZ gene
RC   pLC7-18, pLC13-12 from ColE1 & E. coli flaD gene
RC   pLC10-15, pLC32-9 from ColE1 & E. coli xyl gene
RC   pLC10-26, pLC30-3, pLC30-4 from ColE1 & E. coli dnaZ gene
RC   pLC11-9 from ColE1 & E. coli dnaB gene
RC   pLC13-42 from ColE1 & E. coli chromosome
RC   pLC14-29, pLC26-21 from ColE1 & E. coli his gene
RC   pLC17-38, pLC24-27, pLC30-20 from ColE1 & E. coli recA gene
RC   pLC17-43, pLC18-42, pLC21-33 from ColE1 & E. coli recA/srI gene
RC   pLC22-40, pLC24-32 from ColE1 & E. coli recA/srI gene
RC   pLC20-10, pLC41-13 from ColE1 & E. coli argH genes
RC   pLC20-30 from ColE1 & E. coli lacZ gene
RC   pLC21-2, pLC24-15 from ColE1 & E. coli flaGH/cheB gene
RC   pLC21-33 from ColE1 & E. coli chromosome
RC   pLC21-35, pLC22-3, pLC22-31, pLC26-3 from ColE1 & E. coli ilv gene
RC   pLC27-15, pLC30-15, pLC30-17, pLC44-7 from ColE1 & E. coli ilv gene
RC   pLC23-3, pLC29-5, pLC36-14, pLC41-4 from ColE1 & E. coli cya gene
RC   pLC43-44 from ColE1 & E. coli cya gene
RC   pLC24-16, pLC25-7 from ColE1 & E. coli flaD/hag/flaN gene
RC   pLC24-41 from ColE1 & E. coli araC gene
RC   pLC24-46, pLC35-44, pLC36-11 from ColE1 & E. coli flaKLM gene
RC   pLC25-43 from ColE1 & E. coli dnaE gene
RC   pLC27-20, pLC38-14, pLC38-36 from ColE1 & E. coli mot/cheA/fla gene
RC   pLC28-33, pLC28-34, pLC44-11 from ColE1 & E. coli proA gene
RC   pLC32-33 from ColE1 & E. coli serB/trpR gene
RC   pLC35-1, pLC35-21 from ColE1 & E. coli trpR/thr gene
RC   pLC41-7 from ColE1 & E. coli flaN/flaBCDE gene
RC   pLC44-14 from ColE1 & E. coli lexA gene
RA   Clarke L., Carbon J.;
RT   "A colony bank containing synthetic ColE1 hybrid plasmids
RT   representative of the entire Escherichia coli genome";
RL   Cell 9:91-99(1976).
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CC   A general purpose replicating Saccharomyces cerevisiae/E.coli
CC   shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pLC544)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JA300)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae RH218)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF (pYe[CDC10]1)(pYe[CEN3]41)(pYe35)(pYe[CEN3]30)(pYe6)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1 EcoRI 6646bp 6645..6645
FT                   2. yeast EcoRI-EcoRI 1454bp, ARS1/TRP1 gene
FT                   -> pYe(TRP1)54 24200bp
FT                   1. pBR313 EcoRI 5800bp
FT                   2. pYe(TRP1)54 EcoRI-EcoRI 1454bp, ARS1/TRP1 gene
FT                   -> pLC544 10500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-BglII-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//