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ID   PLD55      preliminary; circular DNA; SYN; 1 BP.
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AC   ATCC20718;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Yarrowia/E.coli plasmid vector pLD55 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pLD40 from pBR322 & LEU2 gene
RC   pLD55 from pLD40 & URA3 gene
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,880,741 dated Nov. 14, 1989.
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CC   Constructed by inserting a Sau3AI (partial) fragment into the BamHI
CC   site of pLD40.
CC   pLD40 is pBR322 containing a 2.3 kb EcoRI fragment (partial) with the
CC   LEU2 gene inserted at the EcoRI site.
CC   Medium is 1245 YEPD.
CC   NM (pLD55)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Yarrowia lipolytica PC-30827 [ATCC 20688])(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. yeast EcoRI-EcoRI 2300bp, LEU2 gene
FT                   -> pLD40 6700bp"
FT                   1. pLD40 BamHI 6700bp 376..376
FT                   2. yeast Sau3AI/MboI-Sau3AI/MboI 1300bp, URA3 gene
FT                   -> pLD55 8000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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