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ID   PLOP205    preliminary; circular DNA; SYN; 89 BP.
AC   M18932;
DT   15-JUN-1989 (Rel. 4, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Saccharomyces plasmid vector pLOP205 - incomplete, fusion region.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-89
RC   [p31Y from pBR322 & PHO5 promoter]
RC   pLOP27.1 from pVR104 & pMBL23
RC   pMBL201 from pMP81 & pBR322
RC   pMBL211 from pMBL201 & pMC1871
RC   pMBL212 from pMBL211 & pUR2
RC   pMBL213 from pMBL211 & pJL1
RC   pMBL214 from pMBL211 & pMT34-317
RC   pMBL215 from pMBL211 & p31Y
RC   pMBL216 from pMBL211 & pMA91
RC   pLOP201 from pMBL211 & pLOP27.1
RC   pLOP202 from pMBL212 & pLOP27.1
RC   pLOP203 from pMBL213 & pLOP27.1
RC   pLOP204 from pMBL214 & pLOP27.1
RC   pLOP205 from pMBL215 & pLOP27.1
RC   pLOP206 from pMBL216 & pLOP27.1
RC   pLOP226 from pLOP206
RA   Verbakel J.M., Dekker K.A., Rutgers C.A., Pouwels P.H.,
RA   Enger-Valk B.E.;
RT   "Construction of expression plasmids for Saccharomyces cerevisiae:
RT   Application for synthesis of poliovirus protein VP2";
RL   Gene 61:207-215(1987).
RN   [2]
RC   pFL1-33 from pFL1 & GAPDH gene
RC   pUR528 from pBR322 & preprothaumatin gene & lacUV5 promoter
RC   pURY528 from pUR528 & yeast 2 micron & LEU2 gene
RC   pURY-528-02, pURY-528-04, pURY-528-05 from pURY528
RC   pURY-522-02 from pUR522
RC   pURY-523-02 from pUR523
RA   Edens L.E., Bom J., Ledeboer A.M., Maat J., Toonen M.Y., Visser C.,
RA   Verrips C.T.;
RT   "Synthesis and processing of the plant thaumatin in yeast";
RL   Cell 37:629-633(1984).
RN   [3]
RC   pMA91 from pMA301 & pMA27
RC   from pMA91 & pCT66
RA   Mellor J., Dobson M.J., Roberts N.A., Tuite M.F., Emtage J.S.,
RA   White S., Lowe P.A., Patel T., Kingsman A.J., Kingsman S.M.;
RT   "Efficient synthesis of enzymatically active calf chymosine in
RT   Saccharomyces cerevisiae";
RL   Gene 24:1-14(1983).
RN   [4]
RC   pYH3003 from pYC1 & lambda gtSc481, GAL7 gene
RC   pYF1016 from pBR322 & lambda gtSc481, GAL7 gene
RC   pMT34-317 from pYH3003, GAL7 promoter
RA   Nogi Y., Fukasawa T.;
RT   "Nucleotide sequence of the transcriptional initiation region of
RT   yeast GAL7 gene";
RL   Nucleic Acids Res. 11:8555-8568(1983).
RN   [5]
RC   pJL1 from CYC1 gene
RA   Planque C.;
RT   ;
RL   Unpublished (1987).
RN   [6]
RC   pBR322/PHO5-HBs from pBR322/PHO5 & pHBV130, HBsAg gene
RC   pBR322/PHO5-HBs delta14 from pBR322/PHO5-HBs
RC   pBR322(TRP1)/PHO5-HBs from pBR322/PHO5-HBs & TRP1
RC   pBR322(TRP1)/PHO5-HBs delta14 from pBR322/PHO5-HBs delta14 & TRP1
RC   pJDB207/PHO5-HBs delta14 from pBR322/PHO5-HBs delta14 & pJDB207
RA   Murray K., Bruce S.A., Hinnen A., Wingfield P., Van Erd P.M.,
RA   De Reus A., Schellekens H.;
RT   "Hepatitis B virus antigens made in microbial cells immunize
RT   against viral infection";
RL   EMBO J. 3:645-650(1984).
RN   [7]
RC   pVR101, pVR103, pVR104, pVR105 from pBR322 & poliovirus 1
RA   Racaniello V.R., Baltimore D.;
RT   "Molecular cloning of poliovirus cDNA and determination of the
RT   complete nucleotide sequence of the viral genome";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:4887-4891(1981).
RN   [8]
RC   pMBL23 from pBR322
RA   Kos A., Broekhuijsen M.P., Van Gorcom R., Van Rotterdam C.,
RA   Enger-Valk B.E., Veltkamp E., Van Den Hondel C., Pouwels P.H.;
RT   "Construction of vectors suitable for efficient expression of genes
RT   in E. coli";
RL   Innovations in Biotechnology 0:415-425(1984).
RL   Houwink E., Van Der Meer R.R., Elsevier, Amsterdam.
RN   [9]
RC   pMA1 from pBR322 & yeast PGK gene
RC   pMA230 from pBR322 & yeast PGK gene
RA   Tuite M.F., Dobson M.J., Roberts N.A., King R.M., Burke D.C.,
RA   Kingsman S.M., Kingsman A.J.;
RT   "Regulated high efficiency expression of human interferon-alpha in
RT   Saccharomyces cerevisiae";
RL   EMBO J. 1:603-608(1982).
RN   [10]
RC   pMA27 or pMA3-PGK from pBR322 & LEU2 gene & yeast PGK gene
RC   pMA301 from pBR322 & yeast 2 micron & LEU2 gene & yeast PGK gene
RA   Dobson M.J., Tuite M.F., Roberts N.A., Kingsman A.J., Kingsman S.M.,
RA   Perkins R.E., Conroy S.C., Dunbar B., Fothergill L.A.;
RT   "Conservation of high efficiency promoter sequences in
RT   Saccharomyces cerevisiae";
RL   Nucleic Acids Res. 10:2625-2637(1982).
RN   [11]
RC   pCT66 from pBR322 & calf prochymosin gene
RC   [pCT1050 from pAT153 & lambda, pL promoter]
RA   Emtage J.S., Angal S., Doel M.T., Harris T.J., Jenkins B., Lilley G.,
RA   Lowe P.A.;
RT   "Synthesis of calf prochymosin (prorennin) in Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:3671-3675(1983).
RN   [12]
RC   lambda gtSc481 from lambda & GAL7 gene
RA   St John T.P., Davis R.W.;
RT   "Isolation of galactose-inducible DNA sequences from Saccharomyces
RT   cerevisiae by differential plaque filter hybridization";
RL   Cell 16:443-452(1979).
RN   [13]
RC   pYC1 from lambda, cos
RA   Hohn B.;
RT   ;
RL   Unpublished (1983).
RN   [14]
RC   pBR322/PHO5 from pBR322 & PHO5 gene
RA   Hinnen A.;
RT   ;
RL   Unpublished (1984).
RN   [15]
RC   pHBV130 from HBV
RA   Gough N.M., Murray K.;
RT   "Expression of the hepatitis B virus surface, core and E antigen
RT   genes by stable rat and mouse cell lines";
RL   J. Mol. Biol. 162:43-67(1982).
RN   [16]
RC   pGAP63 from GAPDH gene
RC   pGAP11 from GAPDH gene
RA   Holland J.P., Labieniec L., Swimmer C., Holland M.J.;
RT   "Homologous nucleotide sequences at the 5' termini of messenger RNAs
RT   synthesized from the yeast enolase and glyceraldehyde-3-phosphate
RT   dehydrogenase genes";
RL   J. Biol. Chem. 258:5291-5299(1983).
RN   [17]
RC   pUR522 from pBR322 & preprothaumatin gene & lacUV5 promoter
RC   pUR523 from pBR322 & preprothaumatin gene & lacUV5 promoter
RA   Edens L.E., Hesinga L., Klok R., Ledeboer A.M., Maat J., Toonen M.Y.,
RA   Visser C., Verrips C.T.;
RT   "Cloning of cDNA encoding the sweet-tasting plant protein thaumatin
RT   and its expression in Escherichia coli";
RL   Gene 18:1-12(1982).
CC   NCBI gi: 209576
CC   NM (pLOP205)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (yeast)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMBL215)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. animal
FT                   poliovirus 7410bp
FT                   1. pBR322 PstI, amp gene
FT                   dC tailing
FT                   2. poliovirus, VP2 seq
FT                   dG tailing
FT                   -> pVR104
FT                   1. pVR104 FokI-PstI 900bp, poliovirus 1 VP2 seq
FT                   oligo FokI-PstI-FokI:
FT                   2. pMBL23 PstI
FT                   -> pLOP27.1
FT                   1. pBR322 BamHI
FT                   2. yeast BamHI-BamHI 500bp, PHO5 gene promoter
FT                   -> p31Y
FT                   1. pMP81 EcoRI-HindIII 3300bp, yeast 2 micron/LEU2
FT                   2. pBR322 EcoRI-SalI 3700bp, ori/amp gene
FT                   3. oligo
FT                   -> pMBL201 7000bp
FT                   1. pMBL201 PstI
FT                   2. pMC1871 PstI-PstI 3100bp, lacZ gene
FT                   -> pMBL211 10100bp
FT                   1. pMBL211 BglII/BamHI, MCS
FT                   2. p31Y BamHI-BamHI 500bp, PHO5 gene promoter
FT                   -> pMBL215 10600bp
FT                   1. pMBL215 PstI
FT                   2. pLOP27.1 PstI-PstI 900bp, poliovirus 1 VP2 sequence
FT                   -> pLOP205 11500bp"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
SQ   Sequence 89 BP; 18 A; 23 C; 27 G; 21 T; 0 other;
     gaattcccgg agatctaagc ttgatatcga gctcatgtgg atccactgca ggtcgacgga
     tccggggaat tcccggggat cccctggtt