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ID   PLUC       preliminary; circular DNA; SYN; 5411 BP.
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AC   U03687; ATCC77414;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli phagemid vector pLUC - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pLUC from pBR322 & phage f1 & lacZ gene & SV40 polyA
RC   pLUCS from pBR322 & phage f1 & lacZ gene & SV40 polyA
RA   Caricasole A., Ward A.D.;
RT   "A luciferase-reporter vector with blue-white selection for rapid
RT   subcloning and mutational analysis of eukaryotic promoters";
RL   Gene 124:139-140(1993).
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RN   [2]
RC   pLUC from pBR322 & phage f1 & lacZ gene & SV40 polyA
RC   pLUCS from pBR322 & phage f1 & lacZ gene & SV40 polyA
RA   Caricasole A., Ward A.D.;
RT   "Transactivation of mouse insulin-like growth factor II (IGF-II)
RT   gene promoters by the AP-1 complex";
RL   Nucleic Acids Res. 21:1873-1879(1993).
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CC   Promoter-cloning vector for analysis of eukaryotic promoters,
CC   using the luciferase reporter gene.
CC   Differs from pLUCS (ATCC 77415) only in the polylinker.
CC   Constructed from pSVOALdelta5' by inserting a 2.6 kb
CC   BamHI fragment, containing the firefly (Photinus pyralis) luciferase
CC   gene and SV40 sequences into the BamHI site of pBluescriptSK+,
CC   followed by the removal of the 3' BamHI site.
CC   Permits beta-galactosidase blue/white color selection for
CC   identification of recombinants.  The color is weaker than from the
CC   parent vector and transformed cells may need to be grown 18-24 hours
CC   at 37C.
CC   The order of the major features in the phagemid is: 3' lacZ -
CC   XhoI/MCS/BamHI - 5' luc 3' - SV40 small t intron -
CC   SV40 polyadenylation signal - NotI - SstII - 5' lacZ - pMB1 ori
CC   - ampR - f1 ori. [1]
CC   Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: A.D.Ward
CC   NM (pLUC)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli)(vertebrate)
CC   CP ()
CC   FN (cloning)(promoter-cloning)(sequencing)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. firefly, luciferase gene 5' deletion
FT                   \ #M15077 [2387bp]
FT                   -> pSVOALdelta5'
FT                   1. pBluescript SK+ BamHI 2964bp 720..720, MCS
FT                   2. pSVOALdelta5' BamHI-BamHI 2600bp, firefly luc gene
FT                   \ #M15077 [2387bp]
FT                   -> plasmid 5600bp
FT                   1. plasmid BamHI 5600bp, 3' to luc gene
FT                   fill in
FT                   -> pLUC 5400bp"
FT   promoter        0..0
FT                   /note="PRO lac"
FT   terminator      0..0
FT                   /note="TER SV40 polyadenylation"
FT   misc_binding    0..0
FT                   /note="MCS XhoI-SalI-AccI-HindIII-PstI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI phage f1"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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