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ID   PLUCS      preliminary; circular DNA; SYN; 5400 BP.
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AC   ATCC77415;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli phagemid vector pLUCS - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pLUC from pBR322 & phage f1 & lacZ gene & SV40 polyA
RC   pLUCS from pBR322 & phage f1 & lacZ gene & SV40 polyA
RA   Caricasole A., Ward A.D.;
RT   "A luciferase-reporter vector with blue-white selection for rapid
RT   subcloning and mutational analysis of eukaryotic promoters";
RL   Gene 124:139-140(1993).
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RN   [2]
RC   pLUC from pBR322 & phage f1 & lacZ gene & SV40 polyA
RC   pLUCS from pBR322 & phage f1 & lacZ gene & SV40 polyA
RA   Caricasole A., Ward A.D.;
RT   "Transactivation of mouse insulin-like growth factor II (IGF-II)
RT   gene promoters by the AP-1 complex";
RL   Nucleic Acids Res. 21:1873-1879(1993).
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CC   Promoter-cloning vector for analysis of eukaryotic promoters,
CC   using the luciferase reporter gene.
CC   Constructed from pLUC (ATCC 77414)
CC   by substitution of a StuI restriction site for the HindIII, PstI and
CC   BamHI sites in the polylinker. Permits beta-galactosidase blue/white
CC   color selection for identification of recombinants.
CC   The color is weaker than from the parent vector and transformed cells
CC   may need to be grown 18-24 hours at 37C.
CC   The order of the major features in the phagemid is:
CC   3' lacZ - XhoI/MCS/StuI - 5' luc 3' - SV40 small t intron - SV40
CC   polyadenylation - NotI - SstII - 5' lacZ - pMB1 ori - ampR - f1 ori.
CC   [1]
CC   Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: A.D.Ward
CC   NM (pLUCS)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli)(vertebrate)
CC   CP ()
CC   FN (cloning)(promoter-cloning)(sequencing)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pLUC remove HindIII-PstI-BamHI 30bp
FT                   \ 690..720, MCS/5400bp
FT                   StuI linker 6bp aggcct
FT                   -> pLUCS 5400bp"
FT   promoter        0..0
FT                   /note="PRO lac"
FT   terminator      0..0
FT                   /note="TER SV40 polyadenylation"
FT   misc_binding    0..0
FT                   /note="MCS XhoI-SalI-AccI-StuI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI phage f1"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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