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ID   PM220      preliminary; circular DNA; SYN; 8200 BP.
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AC   ATCC39853;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pM220 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pM220 from pBR322 & yeast 2 micron & TPI promoter & yeast MFalpha1
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,766,073 dated Aug. 23, 1988.
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CC   Contains EcoRI, PstI, HindIII, BamHI and BglII sites.
CC   A yeast/E.coli shuttle expression vector containing the
CC   Saccharomyces cerevisiae TPI promoter fused to the signal sequence for
CC   MFalpha1 (yeast mating pheromone alpha-factor).
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.2, 1.8, 1.3; HindIII--8.2; BamHI--8.2. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pM220)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. yeast 2 micron, ori
FT                   3. yeast, TPI promoter
FT                   4. yeast, MFalpha1 signal
FT                   -> pM220 8200bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO E. coli TPI gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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